WbaP is required for swarm motility and intramacrophage multiplication of Salmonella Enteritidis spiC mutant by glucose use ability

Wang, Yaonan; GuiFeng, Liu; Zhang, Jian; Gu, Danan; Hu, Mengjie; Zhang, Yunzheng; Pan, Zhiming; Geng, Shizhong; Jiao, Xinan

doi:10.1016/j.micres.2020.126686

Cited by 15 publications

(8 citation statements)

References 36 publications

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“…The wbaP mutant, IM6 isolated from the intestinal tissue of the cefepime treated mice, as well as the E. coli wbaP mutant that we generated for this study, exhibited increased capacity to invade cultured human colonocytes. This phenotype has been observed previously in hypocapsulated Salmonella enteritidis mutants, which although previously implicated in their enhanced ability to invade macrophage cells, were also found to be defective in swarm motility which is important for proliferation in the intracellular environment 66 . It is possible that the intracellular sub-MIC concentration of cefepime would not allow for the formation of intracellular bacterial communities or even for the bacteria to escape their initial phagocytosed membrane-bound organelles remaining in a quiescent state within these vesicles, but further investigation is required to rigorously address these questions.…”

Section: Discussionsupporting

confidence: 78%

Susceptible bacteria survive antibiotic treatment in the mammalian gastrointestinal tract without evolving resistance

Rodrigues

Sabaeifard

Yildiz

et al. 2023

Preprint

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In vitro systems have provided great insight into the mechanisms of antibiotic resistance. Yet, in vitro approaches cannot reflect the full complexity of what transpires within a host. As the mammalian gut is host to trillions of resident bacteria and thus a potential breeding ground for antibiotic resistance, we sought to better understand how gut bacteria respond to antibiotic treatment in vivo. Here, we colonized germ–free mice with a genetically barcoded antibiotic pan–susceptible Escherichia coli clinical isolate and then administered the antibiotic cefepime via programmable subcutaneous pumps which allowed for closer emulation of human parenteral antibiotic pharmacokinetics/dynamics. After seven days of antibiotics, we were unable to culture E. coli from feces. We were, however, able to recover barcoded E. coli from harvested gastrointestinal (GI) tissue, despite high GI tract and plasma cefepime concentrations. Strikingly, these E. coli isolates were not resistant to cefepime but had acquired mutations — most notably in the wbaP gene, which encodes an enzyme required for the initiation of the synthesis of the polysaccharide capsule and lipopolysaccharide O antigen - that increased their ability to invade and survive within intestinal cells, including cultured human colonocytes. Further, these E. coli mutants exhibited a persister phenotype when exposed to cefepime, allowing for greater survival to pulses of cefepime treatment when compared to the wildtype strain. Our findings highlight a mechanism by which bacteria in the gastrointestinal tract can adapt to antibiotic treatment by increasing their ability to persist during antibiotic treatment and invade intestinal epithelial cells where antibiotic concentrations are substantially reduced.

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Section: Discussionsupporting

confidence: 78%

Susceptible bacteria survive antibiotic treatment in the mammalian gastrointestinal tract without evolving resistance

Rodrigues

Sabaeifard

Yildiz

et al. 2023

Preprint

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“…The process of molting hens to obtain another cycle of egg production can be a contributor to shedding and spread within flocks [ 58 ]. As with O-antigen, the flagellar determinants of serotype also undergo tremendous variation that impacts virulence, environmental spread by swarming and swimming, and microbial communication through some branches of quorum sensing [ 59 , 60 ]. Taken together, these lines of research indicate that the evolution of S. enterica to colonize the reproductive tracts of birds and to transmit intergenerationally is a special virulence phenotype that likely requires immense selection pressure to maintain an optimized genome capable of such adaptability.…”

Section: Coordinating Schemes For Communicating S Enterica ...mentioning

confidence: 99%

Through the Looking Glass: Genome, Phenome, and Interactome of Salmonella enterica

Guard

2022

Pathogens

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This review revisits previous concepts on biological phenomenon contributing to the success of the Salmonella enterica subspecies I as a pathogen and expands upon them to include progress in epidemiology based on whole genome sequencing (WGS). Discussion goes beyond epidemiological uses of WGS to consider how phenotype, which is the biological character of an organism, can be correlated with its genotype to develop a knowledge of the interactome. Deciphering genome interactions with proteins, the impact of metabolic flux, epigenetic modifications, and other complex biochemical processes will lead to new therapeutics, control measures, environmental remediations, and improved design of vaccines.

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“…SpiC is an effector protein encoded by SPI-2, which is secreted by the Salmonella type III secretion system (T3SS) and injected into host cells ( 19 , 20 ). It has been demonstrated that deleting spiC gene significantly reduces the virulence of Salmonella in mouse and chicken models ( 21 ).…”

Section: Introductionmentioning

confidence: 99%

Safety of the Salmonella enterica serotype Dublin strain Sdu189-derived live attenuated vaccine—A pilot study

Wang

Wang²,

Ge³

et al. 2022

Front. Vet. Sci.

Self Cite

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Salmonella enterica serovar Dublin (S. Dublin) is an important zoonotic pathogen with high invasiveness. In the prevention and control of the Salmonella epidemic, the live attenuated vaccine plays a very important role. To prevent and control the epidemic of S. Dublin in cattle farms, the development of more effective vaccines is necessary. In this study, we constructed two gene deletion mutants, Sdu189ΔspiC and Sdu189ΔspiCΔaroA, with the parental strain S. Dublin Sdu189. The immunogenicity and protective efficacy were evaluated in the mice model. First, both mutant strains were much less virulent than the parental strain, as determined by the 50% lethal dose (LD50) for specific pathogen-free (SPF) 6-week-old female BALB/c mice. Second, the specific IgG antibody level and the expression level of cytokine TNF-α, IFN-γ, IL-4, and IL-18 were increased significantly in the vaccinated mice compared to the control group. In addition, the deletion strains were cleared rapidly from organs of immunized mice within 14 d after immunization, while the parental strain could still be detected in the spleen and liver after 21 d of infection. Compared with the parental strain infected group, no obvious lesions were detected in the liver, spleen, and cecum of the deletion strain vaccinated groups of mice. Immunization with Sdu189ΔspiC and Sdu189ΔspiCΔaroA both provided 100% protection against subsequent challenges with the wild-type Sdu189 strain. These results demonstrated that these two deletion strains showed the potential as live attenuated vaccines against S. Dublin infection. The present study established a foundation for screening a suitable live attenuated Salmonella vaccine.

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WbaP is required for swarm motility and intramacrophage multiplication of Salmonella Enteritidis spiC mutant by glucose use ability

Cited by 15 publications

References 36 publications

Susceptible bacteria survive antibiotic treatment in the mammalian gastrointestinal tract without evolving resistance

Susceptible bacteria survive antibiotic treatment in the mammalian gastrointestinal tract without evolving resistance

Through the Looking Glass: Genome, Phenome, and Interactome of Salmonella enterica

Safety of the Salmonella enterica serotype Dublin strain Sdu189-derived live attenuated vaccine—A pilot study

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