Water-soluble gold nanoclusters prepared by protein-ligand interaction as fluorescent probe for real-time assay of pyrophosphatase activity

This study demonstrates a one-step synthesis for the preparation of both adenosine monophosphate (AMP)-stabilized colloidal gold nanoparticles (AMP-Au NPs) and fluorescent gold nanoclusters (AMP-Au NCs). The dominant role of AMP:AuCl molar ratios in the formation of diverse nanosized Au products was proved. The size, the structure and the unique structure-dependent optical properties of the NPs and NCs were determined based on the results of numerous spectroscopic (UV-vis, fluorescence, infrared, x-ray photoelectron), high resolution electron microscopy (HRTEM) and dynamic light scattering (DLS) techniques. Stabile AMP-Au NPs with diameter of ca. 11nm and ultra-small AMP-Au NCs having blue fluorescence (λ=480nm) were identified. In addition, the AMP-Au NCs have been utilized to develop a selective sensor for the detection of Fe ions in aqueous medium based on fluorescence quenching. Several essential metal ions and anions have been tested but our results clearly supported that dominant quenching was observed only for Fe ions. Based on the determined limit of detection (LOD=2.0μM) our system is capable of detecting Fe ions in drinking water. The Stern-Volmer constants (K) and various thermodynamic parameters (ΔG, ΔH°, ΔS°, ΔC) of the quenching process have also been determined by the Stern-Volmer fitting of the fluorescence data in order to better understand the quenching mechanism.

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“…"The determined thermodynamic data are summarized in [35]. The detemined ∆C p is -4.86±1.17 kJ mol -1 K -1 .…”

Section: "Having Dls Why Size Distribution Of Np's Is Not Shown?"mentioning

confidence: 99%

“…2a (blue line). The absorption maximum at 260 nm belongs to the purine ring of the AMP and the "shoulder" at 305 nm refers to the formation of the NCs [35].…”

Section: Fluorescence Sensing Of Fe 3+ By Using Amp-au Ncsmentioning

confidence: 99%

Nucleotide-directed syntheses of gold nanohybrid systems with structure-dependent optical features: Selective fluorescence sensing of Fe3+ ions

Ungor

Csapó

Kismárton

et al. 2017

Colloids and Surfaces B: Biointerfaces

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“…As the maximum value of Kq for a diffusion-controlled quenching process is about 2.0 × 10 10 L mol -1 s -1 , we concluded a static quenching mechanism was responsible for the quenching process of BSA caused by ATT-AuNCs [28]. Fluorescence spectroscopy is a most widely used experimental approach to obtain local conformational or dynamic changes of protein, in which tryptophan (Trp) and tyrosine (Tyr) are the main contributors to the endogenous fluorescence [27]. To investigate the interaction between ATT-AuNCs and BSA, fluorescence quenching of BSA upon the addition of ATT-AuNCs was performed.…”

Section: Interaction Between Protein and Att-auncsmentioning

confidence: 91%

“…Since BSA has no fluorescence in this spectral region, the enhancement of fluorescence can be reasonably attributed to the adsorption of ATT-AuNCs on the protein, which results in the restriction of rotation and vibration of the ligands. Fluorescence spectroscopy is a most widely used experimental approach to obtain local conformational or dynamic changes of protein, in which tryptophan (Trp) and tyrosine (Tyr) are the main contributors to the endogenous fluorescence [27]. To investigate the interaction between ATT-AuNCs and BSA, fluorescence quenching of BSA upon the addition of ATT-AuNCs was performed.…”

Section: Interaction Between Protein and Att-auncsmentioning

confidence: 99%

6-Aza-2-Thio-Thymine Stabilized Gold Nanoclusters as Photoluminescent Probe for Protein Detection

et al. 2020

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This study puts forward an efficient method for protein detection in virtue of the tremendous fluorescence enhancement property of 6-aza-2-thio-thymine protected gold nanoclusters (ATT-AuNCs). In-depth studies of the protein-induced photoluminescence enhancement mechanism illustrate the mechanism of the interaction between ATT-AuNCs and protein. This new-established probe enables feasible and sensitive quantification of the concentrations of total protein in real samples, such as human serum, human plasma, milk, and cell extracts. The results of this proposed method are in good agreement with those determined by the classical bicinchoninic acid method (BCA method).

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“…Recently, intracellular PPi in low concentration has become an important indicator for early cancer research [14,15]. Therefore, the development of high-efficient probes for the detection and discrimination of PPi has become an attractive and challenging task in recent years [16][17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Design and Synthesis of a Dinuclear Copper(II) Probe for Selective Fluorescence Sensing of Pyrophosphate

Liu

et al. 2019

Journal of Sensors

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A novel coumarin-based compound DPAC with two dipicolylamine (DPA) arms as the chelator sites was designed and synthesized. The compound DPAC exhibits a highly selective response to Cu2+ ions with a distinctly emission-quenching phenomenon. Moreover, the in situ formed complex DPAC-Cu2+ was used for the detection of pyrophosphate (PPi). The binding manner of probe DPAC-Cu2+ with PPi in 1 : 1 stoichiometry was supported by the Benesi-Hildebrand fitting, ESI-MS and HPLC analysis. The linear range of PPi concentration was 1-4 μM, and the detection limit was 0.53 μM. The competing experiments illustrated that the probe DPAC-Cu2+ had good sensitivity and selectivity for PPi than other anions, including ATP, ADP, AMP, and Pi in CH3CN : HEPES (3 : 2, v/v, pH=7.20) buffer. Further, cell fluorescence imaging experiments indicated that the probe DPAC-Cu2+ had a potential to be used to detect PPi in vivo.

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Water-soluble gold nanoclusters prepared by protein-ligand interaction as fluorescent probe for real-time assay of pyrophosphatase activity

Cited by 68 publications

References 52 publications

Nucleotide-directed syntheses of gold nanohybrid systems with structure-dependent optical features: Selective fluorescence sensing of Fe3+ ions

Nucleotide-directed syntheses of gold nanohybrid systems with structure-dependent optical features: Selective fluorescence sensing of Fe3+ ions

6-Aza-2-Thio-Thymine Stabilized Gold Nanoclusters as Photoluminescent Probe for Protein Detection

Design and Synthesis of a Dinuclear Copper(II) Probe for Selective Fluorescence Sensing of Pyrophosphate

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