6-Aza-2-Thio-Thymine Stabilized Gold Nanoclusters as Photoluminescent Probe for Protein Detection

Abstract

This study puts forward an efficient method for protein detection in virtue of the tremendous fluorescence enhancement property of 6-aza-2-thio-thymine protected gold nanoclusters (ATT-AuNCs). In-depth studies of the protein-induced photoluminescence enhancement mechanism illustrate the mechanism of the interaction between ATT-AuNCs and protein. This new-established probe enables feasible and sensitive quantification of the concentrations of total protein in real samples, such as human serum, human plasma, milk, and cell extracts. The results of this proposed method are in good agreement with those determined by the classical bicinchoninic acid method (BCA method).

Supramolecular Gold Chemistry: From Atomically Precise Thiolate-Protected Gold Nanoclusters to Gold-Thiolate Nanostructures

“…The aim of this Special Issue on "Supramolecular Gold Chemistry" was to provide a unique international forum aimed at covering a broad description of results involving the supramolecular chemistry of gold with a special focus on the gold-sulfur interface leading to hybrid materials, ranging from gold-thiolate complexes, [3] to thiolate-protected gold nanoclusters [4][5][6][7][8][9][10][11] and gold-thiolate supramolecular assemblies or nanoparticles. [12][13][14] The role of thiolates on the structure and optical features of gold nanohybrid systems (ranging from plasmonic gold nanoparticles and fluorescent gold nanoclusters to self-assembled Au-containing thiolated coordination polymers) has been highlighted in the review article by Csapó and coworkers [14].…”

“…Jin and coworkers highlight this molecular-like behavior by thoroughly exploring the differences in the photophysical properties of small organic molecules, gold-thiolate complexes, nanoclusters, and metallic-state nanoparticles [8]. The luminescence properties of 6-aza-2-thio-thymine stabilized gold nanoclusters [9] and gold thiolate coordination polymers [12] demonstrate the high potential of such nanomaterials for bio-sensing or lighting devices. However, in such nanosystems, the origin of photoluminescence (PL) is still not fully understood.…”

Deep Learning-Based Sensor Array: 3D Fluorescence Spectra of Gold Nanoclusters for Qualitative and Quantitative Analysis of Vitamin B6 Derivatives

“…Recently, gold nanoclusters (AuNCs) have been used in the field of sensing construction due to their advantages, which include their stability, ease of preparation, large Stokes shift, and low toxicity. Therefore, such advantages could qualify them for medical and environmental uses. , These applications include protein detection, biomarker identification and monitoring, biological imaging, drug and small molecule detection, heavy metal ion detection, bacteria identification, and others. − Despite the different uses of AuNCs, they are used for sensing in a lock-and-key method (one receptor for one target). Therefore, this method is not suitable for identifying more than one target with similar chemical structures.…”

“…Therefore, such advantages could qualify them for medical and environmental uses. 16,17 These applications include protein detection, biomarker identification and monitoring, biological imaging, drug and small molecule detection, heavy metal ion detection, bacteria identification, and others. 16−24 Despite the different uses of AuNCs, they are used for sensing in a lock-and-key method (one receptor for one target).…”

Feature Selection Assists BLSTM for the Ultrasensitive Detection of Bioflavonoids in Different Biological Matrices Based on the 3D Fluorescence Spectra of Gold Nanoclusters

“…As a result of their unique properties, such as ease of preparation, high stability, large stock shift, and low toxicity, gold nanoclusters (AuNCs) were applied as a sensing probe in many chemo/biosensing applications. − The conventional approach of using AuNCs in sensing is a lock–key approach, but this approach is not suitable to qualify and quantify multiple analytes at the same time, especially for analytes having highly similar structures in complex matrices. , Therefore, the use of the sensor array is the best solution for multiple analyte detection simultaneously as it mimics the smell and taste of the human being . Thus, this approach has different sensitivity and selectivity from one analyte to another, so the sensor array works to collect as many different responses as possible for each analyte separately.…”