Water content of isolated fat cells

Horn, L.W.; Rogus, Ellen M.; Zierler, Kenneth L.

doi:10.1016/0304-4165(73)90041-x

Cited by 8 publications

(7 citation statements)

References 8 publications

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“…However, the membrane-bound TPMP § has been shown to change with ~ in some systems (Bakker, Rottenberg & Caplan, 1976). Therefore, the corrections (Deutsch et al, 1979 b) (Perry & Hales, 1969;Minemura et al, 1970;Gliemann et al, 1972;Zierler, 1972;Horn, Rogus & Zierler, 1973;Horn & Zierler, 1975) and therefore Eq. (i) cannot be used directly to estimate ~,.…”

Section: Theorymentioning

confidence: 95%

Measurement of membrane potentials (ψ) of erythrocytes and white adipocytes by the accumulation of triphenylmethylphosphonium cation

et al. 1980

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The accumulation of the lipophilic cation, triphenylmethylphosphonium, has been employed to determine the resting membrane potential in human erythrocytes, turkey erythrocytes, and rat white adipocytes. The triphenylmethylphosphonium cation equilibrates rapidly in human erythrocytes in the presence of low concentrations of the hydrophobic anion, tetraphenylborate. Tetraphenylborate does not accelerate the uptake of triphenylmethylphosphonium ion by adipocytes. The cell associated vs. extracellular distribution of the triphenylmethylphosphonium ion is proportional to changes in membrane potential. The distribution of this ion reflects the membrane potential determining concentration of the ion with dominant permeability in a "Nernst" fashion. The resting membrane potentials for the human erythrocyte, turkey erythrocyte, and rat white adipocyte were found to be -8.4 +/- 1.3, -16.8 +/- 1.1, and -58.3 +/- 5.0 mV, respectively, values which compare favorably with values obtained by other methods. In addition, changes in membrane potential can be assessed by following triphenylmethylphosphonium uptake without determining the intracellular water space. The method has been successfully applied to a study of hormonally induced changes in membrane potential of rat white adipocytes.

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Section: Theorymentioning

confidence: 95%

Measurement of membrane potentials (ψ) of erythrocytes and white adipocytes by the accumulation of triphenylmethylphosphonium cation

et al. 1980

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“…37 Therefore, water is regarded as an important indicator of breast lesion. Chung et al found the increase of water content in diseased breast tissues with diffuse optical spectroscopy.…”

Section: Effectiveness Of Spectral Analysis Methodsmentioning

confidence: 99%

Visible and near-infrared spectroscopy for distinguishing malignant tumor tissue from benign tumor and normal breast tissuesin vitro

Zhang

Chen

et al. 2013

J. Biomed. Opt

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Abstract. The high incidence and mortality of breast cancer requires an effective, rapid, and cost-effective method for its diagnosis. Here, visible and near-infrared spectroscopy in the wavelength range of 400 to 2200 nm is utilized for distinguishing the malignant tumor tissue from benign tumor and normal breast tissues. Based on the absorption and scattering spectra of fixed samples, three spectral analysis methods are proposed which include an absorption spectral analysis, a scattering spectral analysis, and a combined spectral analysis of the two. By comparison with the histopathological examination, the sensitivity, specificity, and accuracy of the three analysis methods are calculated. The results showed that the combined spectral analysis method can significantly enhance the effectiveness when compared with the sole absorption or scattering spectral analysis method. The sensitivity, specificity, and accuracy of the combined spectral analysis method are 100%, 87.82%, and 87.50% for the benign tumor tissue and 81.82%, 100%, and 87.5% for malignant tumor tissue, respectively. All of the three values are 100% for normal breast tissue. This study demonstrates that the combined spectral analysis method has better potential for in vitro optical diagnosis for breast lesions. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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“…The oil was intended to facilitate the separation of a cellular pellet from the incubation medium. [Horn et al, 1973;Holian et al, 19791. The oil mixtures consisted of various proportions of mineral oil (sp gr 0.86-0.905, Department of Pharmacy, University of California, San Francisco, CA) and Dow Corning 550 fluid (sp gr 1.055-1.075; Anderson Co., Sunnyvale, CA).…”

Section: Methodsmentioning

confidence: 99%

Intracellular volume of osmotically regulated C‐6 glioma cells

Chan¹,

Kerlan²,

Fishman³

1982

J of Neuroscience Research

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The intracellular volume of neoplastic brain cells was investigated with regard to the effects of hypo-osmolality and hyperosmolality utilizing double isotopic labeling with 3-0-methyl-D-glucose or tritiated water to measure the total volume of the pellet and inulin or polyethyleneglycol to measure the extracellular volume of the pellet. The cellular pellets were rapidly separated from the incubation medium by centrifugation after addition of an oil mixture. After 60 minutes incubation in Hanks balanced salt medium, the intracellular volume was 7.50 +/- 0.64, 8.48 +/- 0.19, and 2.97 +/- 0.18 ml H2O per 10(6) packed cells for C-6 glioma cells, N18TG-2 neuroblastoma cells, and NG108-15 neuroblastoma X glioma hybrid cells, respectively. The extracellular trapped space of these cultured cells was about one third of the intracellular volume. The intracellular volume of C-6 glioma cells was increased in hypotonic environment, whereas it was decreased with hyperosmolality. Both intracellular sodium and potassium were increased with increased osmolality of the incubation media. These data indicate iso-osmotic regulation by tumor cells, i.e., there is a good correlation between the intracellular volume, intracellular cations and lactate levels of C-6 glioma cells under various osmotic conditions.

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Water content of isolated fat cells

Cited by 8 publications

References 8 publications

Measurement of membrane potentials (ψ) of erythrocytes and white adipocytes by the accumulation of triphenylmethylphosphonium cation

Measurement of membrane potentials (ψ) of erythrocytes and white adipocytes by the accumulation of triphenylmethylphosphonium cation

Visible and near-infrared spectroscopy for distinguishing malignant tumor tissue from benign tumor and normal breast tissuesin vitro

Intracellular volume of osmotically regulated C‐6 glioma cells

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