Visible and near-infrared spectroscopy for distinguishing malignant tumor tissue from benign tumor and normal breast tissuesin vitro

Abstract

Abstract. The high incidence and mortality of breast cancer requires an effective, rapid, and cost-effective method for its diagnosis. Here, visible and near-infrared spectroscopy in the wavelength range of 400 to 2200 nm is utilized for distinguishing the malignant tumor tissue from benign tumor and normal breast tissues. Based on the absorption and scattering spectra of fixed samples, three spectral analysis methods are proposed which include an absorption spectral analysis, a scattering spectral analysis, and a combined spectral analysis of the two. By comparison with the histopathological examination, the sensitivity, specificity, and accuracy of the three analysis methods are calculated. The results showed that the combined spectral analysis method can significantly enhance the effectiveness when compared with the sole absorption or scattering spectral analysis method. The sensitivity, specificity, and accuracy of the combined spectral analysis method are 100%, 87.82%, and 87.50% for the benign tumor tissue and 81.82%, 100%, and 87.5% for malignant tumor tissue, respectively. All of the three values are 100% for normal breast tissue. This study demonstrates that the combined spectral analysis method has better potential for in vitro optical diagnosis for breast lesions. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

Diffuse optical mammotomography: state-of-the-art and prospects

“…In this case, one can clearly see the increasing contribution of sufficiently small scatterers to the formation of the scattering spectrum, which is a manifestation of destruction of structure elements of the breast tissue with the development of the neoplasm. In the spectral range from 1000 to 2200 nm, no essential change in the light scattering is observed [192].…”

“…However, as shown in Ref. [192], the measurements carried out in the near IR spectral range, in the region of absorption bands of water ( max ~1450 nm) and lipids ( max ~1720 nm) allow reliable differentiation of normal tissue, benign and malignant neoplasms. Thus, at the wavelength 1450 nm the absorption coefficient of the normal tissue amounts to 0.6686  0. which is a sign of increasing content of water in the tumour in the course of its development.…”

“…In this field the main stages were associated with the spectrophotometry using integrating spheres [1,164,180,[188][189][190][191][192] and the development of iteration methods, such as inverse adding-doubling method [180,192,193], and the inverse Monte Carlo method [190]. Among the methods of determining the optical parameters of tissues, the most widespread one is the method of integrating spheres.…”

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“…Therefore, both tissue optical parameters and diffuse reflectance profile can powerfully assist in the process of medical diagnosis and therapy monitoring [6,13–15]. Visible and near infrared diffuse optical spectroscopic techniques have been widely used in breast cancer diagnosis process [16,17] and in differentiating malignant tumor tissue from benign tumor and normal breast tissues [18,19].…”

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