Intracellular volume of osmotically regulated C‐6 glioma cells

Abstract

The intracellular volume of neoplastic brain cells was investigated with regard to the effects of hypo-osmolality and hyperosmolality utilizing double isotopic labeling with 3-0-methyl-D-glucose or tritiated water to measure the total volume of the pellet and inulin or polyethyleneglycol to measure the extracellular volume of the pellet. The cellular pellets were rapidly separated from the incubation medium by centrifugation after addition of an oil mixture. After 60 minutes incubation in Hanks balanced salt medium, the intracellular volume was 7.50 +/- 0.64, 8.48 +/- 0.19, and 2.97 +/- 0.18 ml H2O per 10(6) packed cells for C-6 glioma cells, N18TG-2 neuroblastoma cells, and NG108-15 neuroblastoma X glioma hybrid cells, respectively. The extracellular trapped space of these cultured cells was about one third of the intracellular volume. The intracellular volume of C-6 glioma cells was increased in hypotonic environment, whereas it was decreased with hyperosmolality. Both intracellular sodium and potassium were increased with increased osmolality of the incubation media. These data indicate iso-osmotic regulation by tumor cells, i.e., there is a good correlation between the intracellular volume, intracellular cations and lactate levels of C-6 glioma cells under various osmotic conditions.

Breast cancer resistance protein (BCRP/ABCG2) localises to the nucleus in glioblastoma multiforme cells

“…When LN229 cells were incubated in the presence of 5 μM FTC, the drug readily accumulated in the cytosol (Figure 4). The intracellular concentration of FTC was determined to be 32 μM after adjusting for the intracellular volume (0.375 μl for 50×10 3 cells (Chan et al ., 1982)). Therefore, it is likely that FTC will also be able to inhibit the export function of BCRP located within the nuclear membrane.…”