Water binding and mobility in the phosphatidylcholine/cholesterol/water lamellar phase

Inglefield, Paul T.; Lindblom, Kenneth A.; Gottlieb, Albert M.

doi:10.1016/0005-2736(76)90346-1

Cited by 17 publications

(10 citation statements)

References 17 publications

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“…The ␣-gel phase changed to a liquid crystalline phase because of cholesteric effect [17]. This phase transition further increased the water holding capacity of the lamellar structure [18].…”

Section: Resultsmentioning

confidence: 99%

A liquid crystal application in skin care cosmetics

Iwai

Fukasawa

Suzuki

1998

Intern J of Cosmetic Sci

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Intercellular lipids of the stratum corneum contribute threefold to the maintenance of a healthy skin: by hydration, cell adhesion, and reduction of transepidermal water loss. All of these functions can be attributed to the self-organizing property of the amphiphilic molecules of the stratum corneum lipids. A new type of skin care product called Lamellar Gel was developed, which contains a (synthesized) pseudo-ceramide. Its structure is similar to that of ceramide found among the stratum corneum lipids, which allows it to control intramolecular interactions. Compared to regular emulsions the Lamellar Gel demonstrated better skin care characteristics regarding permeability, skin hydration, and skin occlusion. This was attributed to the fact that it formed the same self-organizing structure as natural stratum corneum lipids, hence showing a high affinity to the skin. A high moisturizing effect was observed as the Lamellar Gel combines the benefits of both O/W and W/O emulsions: it provides the same initial hydration as an O/W emulsion and at the same time the same occlusivity as a W/O emulsion. Transepidermal water loss increases under dry environmental conditions. This especially affects the skin around the eyes, where the skin is very thin, and wrinkles are very easily formed. Treatment with the Lamellar Gel recovered these wrinkles promptly and hydrated the stratum corneum for a long time.

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Section: Resultsmentioning

confidence: 99%

A liquid crystal application in skin care cosmetics

Iwai

Fukasawa

Suzuki

1998

Intern J of Cosmetic Sci

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“…especially as separate measurements of, for example, the partition coefficient Kp and the core-diffusion constant D m are missing. The problem is even more complex as the lipid bilayer is surrounded by water layers with different properties than those of the bulk water (Rigaud, GaryBobo & Lange, 1972;Salsbury, Darke & Chapman, 1972;Cornell, Pope & Troup, 1974;Finer & Darke, 1974;Finch & Schneider, 1975;Inglefield, Lindblom & Gottlieb, 1976;Jendrasiak & Mendible, 1976;Keith, Snipes & Chapman, 1977). The penetration depth of the adjacent water layer is almost unknown on a quantitative basis and may be a function of the temperature, the lipid, and additionally, in natural membranes, of the protein composition.…”

Section: + (11) P~ Ds Dkpmentioning

confidence: 97%

On the permeability of water molecules across vesicular lipid bilayers

Lawaczeck

1979

J. Membrain Biol.

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Summary. The permeation of water molecules across single-component lecithin or lecithin-cholesterol bilayers is studied by a new technique. The new technique makes use of the different fluorescencc quantum yields of appropriate molecules in D20 and H20. Water-soluble indole derivatives which by experimental manipulation residc almost entirely within the aqueous (H20) intravcsicular compartment thus can monitor D20 molecules permeating the bilayer by virtue of an increased quantum yield of the fluorescence. In a stopped-flow instrument, a vesicle solution containing the fluorescent chromophore in the intravesicular space is rapidly mixed with the deuterated solvent. The approach to the "steady state"~ where the intra-and extravesicular D20 and H20 concentrations are equal, proceeds in a single-exponcntial manner. Consequently, the exchange relaxation time for the DxO molecules passing the bilayer can be deduced from the time-dependent increase of the tluorescence intensity. The method and results on lecithin and lecithin-cholesterol bilayer vesicles are discussed. The exchange relaxation times of temperature-dependent studies are interpreted within the framework of the solubility-diffusion theory. Below the crystalline to liquid-crystalline phase transition temperature and for cholesterol-free vesicles, the rate-limiting step for the D20 permeation is attributed to the intracore diffusion. Above the phase transition and for cholesterol-containing vesicles, the intracore diffusion seems not to be rate-limiting. Deviations from the linearity below the phase transition in the Arrhenius-type presentation of the data are related to changes of the partition coefficient of water between the solvent and the lipid phase at the premelting temperature.Intracellular water is coupled to the external milieu via the permeation of water molecules through the plasma membrane. This permeation is an attracting and substantial process, and different physical methods were developed to measure this step. The water transport across model membranes in the form of bilayer vesicles (Reeves & Dowben,

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“…(g) The water binding capacity of egg phosphatidylcholine bilayers is affected by cholesterol as shown by a variety of techniques (35,(43)(44)(45). For example, the hydration (moles of water per mole of phospholipid) is 6.5 for the hydrophilic headgroup of pure egg phosphatidylcholine vesicles, whereas for egg phosphatidylcholine vesicles containing 30 mote % cholesterol the hydration increases to 9.0 (45).…”

Section: Cholesterol Phosphatidy|choline Bilayersmentioning

confidence: 99%

A structural model for the cholesterol‐phosphatidylcholine complexes in bilayer membranes

Huang

1977

Lipids

216

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Based on the structural properties of phospholipid and cholesterol molecules, and making use of the known structural and motional effects of cholesterol and its analogs on phospholipid bilayers, a model for the cholesterol-phosphatidylcholine complex is proposed. In this model, the 3beta-hydroxyl group of cholesterol is assumed to engage in hydrogen bonding with the carbonyl oxygen of the fatty acyl groups in phospholipids. Some specific configurations of the saturated and unsaturated fatty acyl chains of the phospholipid are suggested to participate in van der Waals attractive interactions with the apha and beta surface of the steroid nucleus.

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Water binding and mobility in the phosphatidylcholine/cholesterol/water lamellar phase

Cited by 17 publications

References 17 publications

A liquid crystal application in skin care cosmetics

A liquid crystal application in skin care cosmetics

On the permeability of water molecules across vesicular lipid bilayers

A structural model for the cholesterol‐phosphatidylcholine complexes in bilayer membranes

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