VP37 of white spot syndrome virus interact with shrimp cells

Liu, Q.-H.; Zhang, X.-L.; Ma, Cuiyan; Liang, Yan; Huang, Jie

doi:10.1111/j.1472-765x.2008.02482.x

Cited by 26 publications

(12 citation statements)

References 17 publications

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“…It seems that the blocking effect is incomplete when only one single protein is used. This suggested that the virus infection event is mediated by multiple host proteins [10,25]. The possibility of other host proteins participating in WSSV infection stays unclear and also has to be taken into further consideration.…”

Section: Discussionmentioning

confidence: 96%

Penaeus monodon chitin-binding protein (PmCBP) is involved in white spot syndrome virus (WSSV) infection

Chen

Hsu

Huang

et al. 2009

Fish & Shellfish Immunology

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Section: Discussionmentioning

confidence: 96%

Penaeus monodon chitin-binding protein (PmCBP) is involved in white spot syndrome virus (WSSV) infection

Chen

Hsu

Huang

et al. 2009

Fish & Shellfish Immunology

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“…The fourth external protein in this complex, VP37, contains an RGD motif [43]. RGD is a sequence that is associated with cell attachment, and VP37 has been shown to attach to shrimp cell membranes [18], [45]. Among the proteins on the inside of the envelope, VP26 may be instrumental in trafficking the WSSV nucleocapsid into the host nucleus via the cytoskeleton [34].…”

Section: Discussionmentioning

confidence: 99%

A 3D Model of the Membrane Protein Complex Formed by the White Spot Syndrome Virus Structural Proteins

et al. 2010

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BackgroundOutbreaks of white spot disease have had a large negative economic impact on cultured shrimp worldwide. However, the pathogenesis of the causative virus, WSSV (whit spot syndrome virus), is not yet well understood. WSSV is a large enveloped virus. The WSSV virion has three structural layers surrounding its core DNA: an outer envelope, a tegument and a nucleocapsid. In this study, we investigated the protein-protein interactions of the major WSSV structural proteins, including several envelope and tegument proteins that are known to be involved in the infection process.Principal FindingsIn the present report, we used coimmunoprecipitation and yeast two-hybrid assays to elucidate and/or confirm all the interactions that occur among the WSSV structural (envelope and tegument) proteins VP51A, VP19, VP24, VP26 and VP28. We found that VP51A interacted directly not only with VP26 but also with VP19 and VP24. VP51A, VP19 and VP24 were also shown to have an affinity for self-interaction. Chemical cross-linking assays showed that these three self-interacting proteins could occur as dimers.ConclusionsFrom our present results in conjunction with other previously established interactions we construct a 3D model in which VP24 acts as a core protein that directly associates with VP26, VP28, VP38A, VP51A and WSV010 to form a membrane-associated protein complex. VP19 and VP37 are attached to this complex via association with VP51A and VP28, respectively. Through the VP26-VP51C interaction this envelope complex is anchored to the nucleocapsid, which is made of layers of rings formed by VP664. A 3D model of the nucleocapsid and the surrounding outer membrane is presented.

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“…In this study, the results of far-western showed that rFcβInt-ER was able to recognize the rVP31, rVP37, rVP110 and rVP187, which all contain cell attachment RGD motif. Among them, VP37 is an important envelope protein of WSSV [ 19 ], which plays a major role in WSSV infection [ 20 ], and the recombinant VP37 has shown a high binding activity with the shrimp cell membrane in a binding assay [ 21 ]. VP31 is also an important viral envelope protein that might be involved in WSSV infection [ 22 ], and was identified as being able to interact with several proteins in shrimp [ 23 , 24 ].…”

Section: Discussionmentioning

confidence: 99%

The Roles of β-Integrin of Chinese Shrimp (Fenneropenaeus chinensis) in WSSV Infection

Tang

Zhai

Sheng

et al. 2017

IJMS

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Our previous study demonstrated that an integrin β subunit of Chinese shrimp (Fenneropenaeus chinensis) (FcβInt) plays an important role in white spot syndrome virus (WSSV) infection. In the present work, in order to further elucidate the potential role of FcβInt in WSSV infection, the recombinant extracellular domain of β integringene of F. Chinensis (rFcβInt-ER) was expressed in Escherichia coli BL21 (DE3), and the eukaryotic expression plasmid PcDNA3.1-FcβInt-ER (PFcβInt-ER) was also constructed. Far-western blotting was performed to determine the binding specificity of rFcβInt-ER to WSSV envelope proteins, and results showed that rFcβInt-ER was able to specifically interact with rVP31, rVP37, rVP110 and rVP187. Moreover, the blocking effects of mouse anti-rFcβint-ER antibodies were both detected in vivo and in vitro. The ELISA and Dot-blotting in vitro assays both showed that mouse anti-rFcβInt-ER antibodies could partially block the binding of WSSV to the hemocyte membrane of F. chinensis. In the in vivo assays, the mortality of shrimp injected with WSSV mixed with anti-rFcβInt-ER antibodies was delayed, and was lower than in the control group. While the shrimp were intramuscularly injected with PFcβInt-ER, transcripts of PFcβInt-ER could be detected in different shrimp tissues within 7 days, and the mortality of shrimp injected with PFcβInt-ER was also delayed and lower compared with the control group post WSSV challenge. Furthermore, gene silencing technology was also used to verify the effect of FcβInt in WSSV infection, and results showed that the expression levels of the WSSV immediate early gene iel, early gene wsv477, and late gene VP28 and the mortality of F. Chinensis were all significantly decreased in the FcβInt knock-down hemocyctes compared to the control group. Taken together, these results suggest that FcβInt plays important roles in WSSV infection.

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VP37 of white spot syndrome virus interact with shrimp cells

Cited by 26 publications

References 17 publications

Penaeus monodon chitin-binding protein (PmCBP) is involved in white spot syndrome virus (WSSV) infection

Penaeus monodon chitin-binding protein (PmCBP) is involved in white spot syndrome virus (WSSV) infection

A 3D Model of the Membrane Protein Complex Formed by the White Spot Syndrome Virus Structural Proteins

The Roles of β-Integrin of Chinese Shrimp (Fenneropenaeus chinensis) in WSSV Infection

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