The Roles of β-Integrin of Chinese Shrimp (Fenneropenaeus chinensis) in WSSV Infection

Abstract

Our previous study demonstrated that an integrin β subunit of Chinese shrimp (Fenneropenaeus chinensis) (FcβInt) plays an important role in white spot syndrome virus (WSSV) infection. In the present work, in order to further elucidate the potential role of FcβInt in WSSV infection, the recombinant extracellular domain of β integringene of F. Chinensis (rFcβInt-ER) was expressed in Escherichia coli BL21 (DE3), and the eukaryotic expression plasmid PcDNA3.1-FcβInt-ER (PFcβInt-ER) was also constructed. Far-western blotting was performed to determine the binding specificity of rFcβInt-ER to WSSV envelope proteins, and results showed that rFcβInt-ER was able to specifically interact with rVP31, rVP37, rVP110 and rVP187. Moreover, the blocking effects of mouse anti-rFcβint-ER antibodies were both detected in vivo and in vitro. The ELISA and Dot-blotting in vitro assays both showed that mouse anti-rFcβInt-ER antibodies could partially block the binding of WSSV to the hemocyte membrane of F. chinensis. In the in vivo assays, the mortality of shrimp injected with WSSV mixed with anti-rFcβInt-ER antibodies was delayed, and was lower than in the control group. While the shrimp were intramuscularly injected with PFcβInt-ER, transcripts of PFcβInt-ER could be detected in different shrimp tissues within 7 days, and the mortality of shrimp injected with PFcβInt-ER was also delayed and lower compared with the control group post WSSV challenge. Furthermore, gene silencing technology was also used to verify the effect of FcβInt in WSSV infection, and results showed that the expression levels of the WSSV immediate early gene iel, early gene wsv477, and late gene VP28 and the mortality of F. Chinensis were all significantly decreased in the FcβInt knock-down hemocyctes compared to the control group. Taken together, these results suggest that FcβInt plays important roles in WSSV infection.

iTRAQ-based quantitative proteomic analysis of differentially expressed proteins inLitopenaeus vannameiin response to infection with WSSV strains varying in virulence

“…; Tang et al . ). Glycerol‐3‐phosphate dehydrogenase is a metabolic enzyme of carbohydrate, and the enrichment of this protein can reflect the effect of WSSV infection on metabolic pathway.…”

Metamorphism and P-T Evolution of High-Pressure Granulites from the Fuping Complex, North China Craton

“…The North China Craton (NCC) is one of the oldest cratonic blocks in the world, preserving records of Archean to late Palaeoproterozoic crustal evolution, cratonization, and related geodynamic history that are important in understanding continental growth in the early Earth [1][2][3][4][5][6][7]. Based on differences in geochronology, rock assemblages, and P-T paths [8,9], the Precambrian basement within the NCC has been subdivided into several tectonic units, including the Western Block (WB), Eastern Block (EB), and the intervening Trans-North China Orogen (TNCO; [6,7]).…”

Prediction of Interaction Sites of PcRab7-VP28

“…Among these structural proteins, envelope protein is an important determinant of viral infection and pathogenicity [4], which is considered to be the first molecule in direct contact with the host [5] [6], so it plays a key role in cell targeting and host defense. Although the exact mechanism of WSSV virus entry into shrimp is still unknown, it is speculated that the virus entry into host cells is through the interaction between virus envelope protein and host cell receptor [7]. Since the virus enters the host cell through the interaction between the virus envelope protein and the host cell receptor, it is very important for the discovery of interaction blockers, especially for the development of antiviral drugs [8].…”