Penaeus monodon chitin-binding protein (PmCBP) is involved in white spot syndrome virus (WSSV) infection

Chen, Kuan‐Yu; Hsu, Tai Ching; Huang, Po Yu; Kang, Shih Ting; Lo, Chu Fang; Huang, Wei Pang; Chen, Li Li

doi:10.1016/j.fsi.2009.06.018

Cited by 77 publications

(65 citation statements)

References 25 publications

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“…The WSSV inoculum and test solution were prepared based on a previously described method [17]. Briefly, 100 ml of haemolymph was withdrawn from WSSV-infected white shrimp L. vannamei and diluted with 400 ml of PBS.…”

Section: Preparation Of Wssvmentioning

confidence: 99%

White shrimp Litopenaeus vannamei immersed in seawater containing Sargassum hemiphyllum var. chinense powder and its extract showed increased immunity and resistance against Vibrio alginolyticus and white spot syndrome virus

Huynh

Yeh

Shyu

et al. 2011

Fish & Shellfish Immunology

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Section: Preparation Of Wssvmentioning

confidence: 99%

White shrimp Litopenaeus vannamei immersed in seawater containing Sargassum hemiphyllum var. chinense powder and its extract showed increased immunity and resistance against Vibrio alginolyticus and white spot syndrome virus

Huynh

Yeh

Shyu

et al. 2011

Fish & Shellfish Immunology

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“…The beta-integrin from M. japonicas could directly interact with envelope protein VP187 of WSSV (Li et al, 2007). One chitinbinding protein (PmCBP) from Penaeus monodon was suggested to be a cellular receptor or co-receptor for WSSV through interaction with the envelope protein VP53A of WSSV (Chen et al, 2009). One C-type lectin from Litopenaeus vannamei was reported to have strong affinity to WSSV through interacting with several envelope proteins of WSSV (Zhao et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Analysis on the expression and function of syndecan in the Pacific white shrimp Litopenaeus vannamei

Yang

et al. 2015

Developmental & Comparative Immunology

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“…Interestingly, VP24 was recently found to interact with the Penaeus monodon CBP PmCBP, which exists in different shrimp species (27) and is widely expressed in many tissues, including the stomach and midgut (41). In nature, CBPs are the major group of proteins that associate with chitin and participate in the formation, maintenance, and regulation of the functions of these extracellular structures (42).…”

Section: Discussionmentioning

confidence: 99%

“…Although the function of PmCBP remains unclear, depletion of PmCBP by double-stranded RNA (dsRNA) interference (27) or neutralization of PmCBP with recombinant protein (41) leads to an increase in the shrimp survival rate after WSSV challenge. Because PmCBP has been found on the cell surface and can interact with six WSSV envelope proteins, including VP24, it is believed to be a good receptor candidate for WSSV (27,41). Combined with the results of the present study, we speculate that VP24 is a key factor involved in WSSV infection.…”

Section: Discussionmentioning

confidence: 99%

VP24 Is a Chitin-Binding Protein Involved in White Spot Syndrome Virus Infection

Han

et al. 2016

J Virol

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Oral ingestion is the major route of infection for the white spot syndrome virus (WSSV). However, the mechanism by which virus particles in the digestive tract invade host cells is unknown. In the present study, we demonstrate that WSSV virions can bind to chitin through one of the major envelope proteins (VP24). Mutagenesis analysis indicated that amino acids (aa) 186 to 200 in the C terminus of VP24 were required for chitin binding. Moreover, the P-VP24 186 -200 peptide derived from the VP24 chitin binding region significantly inhibited the VP24-chitin interaction and the WSSV-chitin interaction, implying that VP24 participates in WSSV binding to chitin. Oral inoculation experiments showed that P-VP24 186 -200 treatment reduced the number of virus particles remaining in the digestive tract during the early stage of infection and greatly hindered WSSV proliferation in shrimp. These data indicate that binding of WSSV to chitin through the viral envelope protein VP24 is essential for WSSV per os infection and provide new ideas for preventing WSSV infection in shrimp farms. IMPORTANCEIn this study, we show that WSSV can bind to chitin through the envelope protein VP24. The chitin-binding domain of VP24 maps to amino acids 186 to 200 in the C terminus. Binding of WSSV to chitin through the viral envelope protein VP24 is essential for WSSV per os infection. These findings not only extend our knowledge of WSSV infection but also provide new insights into strategies to prevent WSSV infection in shrimp farms. W hite spot syndrome virus (WSSV) is an enveloped doublestranded DNA (dsDNA) virus belonging to the genus Whispovirus, family Nimaviridae (1). WSSV has caused enormous economic losses to the shrimp farming industry since 1993. Infectious WSSV virions are typically 250 to 380 nm in length and 120 to 150 nm in diameter (2, 3), and they contain a genome of ϳ300 kb that encodes ϳ180 proteins (4, 5).Ingestion of WSSV-infected sick or dead shrimp has been accepted as the major route of natural infection due to the cannibalistic nature of shrimp (6-11). Therefore the digestive tract of shrimp may be a primary site of infection. The digestive tract of shrimp is composed of the esophagus, stomach, midgut, and hindgut. The esophagus, stomach, and hindgut possess a chitinous lining, which is not present in the midgut (12, 13). Instead, the midgut epithelium is generally lined with the peritrophic membrane (PM), which is a noncellular structure surrounding the food bolus. The PM is composed of regularly arranged chitin fibrils embedded in a matrix of proteins, proteoglycans, and mucopolysaccharides (14-17). Therefore, WSSV must cross the PM in the midgut or the chitinous lining in the other parts of the digestive tract to traverse the basal membranes and reach the host cells. We speculate that the interaction between WSSV and chitin may be important for WSSV infection in the shrimp digestive tract.More than 40 WSSV structural proteins have been identified using proteomic methods to date (18)(19)(20). Among them, VP28,...

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Penaeus monodon chitin-binding protein (PmCBP) is involved in white spot syndrome virus (WSSV) infection

Cited by 77 publications

References 25 publications

White shrimp Litopenaeus vannamei immersed in seawater containing Sargassum hemiphyllum var. chinense powder and its extract showed increased immunity and resistance against Vibrio alginolyticus and white spot syndrome virus

White shrimp Litopenaeus vannamei immersed in seawater containing Sargassum hemiphyllum var. chinense powder and its extract showed increased immunity and resistance against Vibrio alginolyticus and white spot syndrome virus

Analysis on the expression and function of syndecan in the Pacific white shrimp Litopenaeus vannamei

VP24 Is a Chitin-Binding Protein Involved in White Spot Syndrome Virus Infection

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