Voluntary wheel running attenuates lipopolysaccharide-induced liver inflammation in mice

Peppler, Willem T.; Anderson, Zachary; Sutton, Charles D.; Rector, R. Scott; Wright, David C.

doi:10.1152/ajpregu.00497.2015

Cited by 27 publications

(37 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The mice used in this study represent a subset of a prior publication from our group. 21 All mice had ad libitum access to standard rodent chow from Teklad (Madison, WI; Cat # 7004) and water, were on a 12-hour light dark cycle (9 A.M. to 9 P.M.), and were housed at »22 C. Running wheel distances were counted daily using a wheel mounted rotation counter (VDO M3 wired bike computer, Mountain Equipment Co-Op Vancouver, Canada). Food intake was measured weekly during the 10 week period using the amount consumed from the hopper.…”

Section: Animalsmentioning

confidence: 99%

“…For example, we have recently shown that mice given access to a voluntary running wheel, a model of physical activity, were protected against LPS induced inflammation in the liver. 21 This effect was predominantly evident at 6 hours versus 12 hours post LPS, which is a time frame at which systemic levels of pro-inflammatory factors peak. 5 Despite these studies, [19][20][21][22] little is known if a similar protective effect is evident in adipose tissue.…”

Section: Introductionmentioning

confidence: 99%

“…21 This effect was predominantly evident at 6 hours versus 12 hours post LPS, which is a time frame at which systemic levels of pro-inflammatory factors peak. 5 Despite these studies, [19][20][21][22] little is known if a similar protective effect is evident in adipose tissue. Recently, using a cross sectional design, it was reported that persons who were exercise trained (VO 2 max >55 ml¢kg ¡ 1¢min…”

Section: Introductionmentioning

confidence: 99%

“…There is data to suggest that physical activity [19][20][21] or acute exercise 22 can modulate the response to LPS. For example, we have recently shown that mice given access to a voluntary running wheel, a model of physical activity, were protected against LPS induced inflammation in the liver.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Habitual physical activity protects against lipopolysaccharide-induced inflammation in mouse adipose tissue

et al. 2016

Self Cite

View full text Add to dashboard Cite

Sepsis is a systemic inflammatory response to infection, with no preventative strategies. In this study, we identify a role for habitual physical activity in the prevention of adipose tissue inflammation induced by a model of sepsis, lipopolysaccharide (LPS). Male C57BL/6J mice (8 weeks old) were housed with access to voluntary wheel running (VWR) or sedentary (SED) for 10 weeks. Mice were then injected with LPS (2 mg/kg) or saline (SAL), and tissues were removed 6 hours postinjection. VWR attenuated body, epididymal adipose tissue (eWAT), and subcutaneous inguinal adipose tissue (iWAT) mass gain, improved glucose tolerance, increased markers of mitochondrial biogenesis in iWAT and eWAT, and increased UCP-1 protein content in iWAT. In iWAT, VWR attenuated the LPS induced increase in mRNA expression of TNF-a, MCP-1, and follistatin, along with phosphorylation of STAT3. In addition, VWR had a main effect for reducing iWAT mRNA expression of IL-1b, IL-6, and SOCS3. In eWAT, VWR had a main effect for reducing mRNA expression of IL-1b, MCP-1, IL-6, and follistatin. Further, VWR increased SOCS3 mRNA expression and phosphorylation of STAT3 in SAL mice, thus the relative change in response to LPS for these markers was attenuated. The protective effect of prior physical activity occurred in conjunction with increases in the protein content of a component of the LPS binding complex, MyD88. Overall, the results from this study demonstrate that habitual physical activity can attenuate the LPS induced inflammatory response in adipose tissue and this occurs to a greater extent in iWAT compare with eWAT.

show abstract

Section: Animalsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Habitual physical activity protects against lipopolysaccharide-induced inflammation in mouse adipose tissue

et al. 2016

Self Cite

View full text Add to dashboard Cite

show abstract

“…While influenced by differences in stimulation concentrations, incubation time, and cell preparation,19 ex vivo stimulation by lipopolysaccharide nonetheless may more accurately reflect immune regulation 20. Many human21, 22 and animal23, 24, 25 studies (though not all—see below) report the predicted anti‐inflammatory effects of exercise training on lipopolysaccharide‐inducible cytokine release.…”

mentioning

confidence: 99%

Aerobic Exercise Training and Inducible Inflammation: Results of a Randomized Controlled Trial in Healthy, Young Adults

Sloan

Shapiro

McKinley

et al. 2018

JAHA

View full text Add to dashboard Cite

BackgroundConsensus panels regularly recommend aerobic exercise for its health‐promoting properties, due in part to presumed anti‐inflammatory effects, but many studies show no such effect, possibly related to study differences in participants, interventions, inflammatory markers, and statistical approaches. This variability makes an unequivocal determination of the anti‐inflammatory effects of aerobic training elusive.Methods and ResultsWe conducted a randomized controlled trial of 12 weeks of aerobic exercise training or a wait list control condition followed by 4 weeks of sedentary deconditioning on lipopolysaccharide (0, 0.1, and 1.0 ng/mL)‐inducible tumor necrosis factor‐α (TNF‐α) and interleukin‐6 (IL‐6), and on toll‐like receptor 4 in 119 healthy, sedentary young adults. Aerobic capacity by cardiopulmonary exercise testing was measured at study entry (T1) and after training (T2) and deconditioning (T3). Despite a 15% increase in maximal oxygen consumption, there were no changes in inflammatory markers. Additional analyses revealed a differential longitudinal aerobic exercise training effect by lipopolysaccharide level in inducible TNF‐α (P=0.08) and IL‐6 (P=0.011), showing T1 to T2 increases rather than decreases in inducible (lipopolysaccharide 0.1, 1.0 versus 0.0 ng/mL) TNF‐α (51% increase, P=0.041) and IL‐6 (42% increase, P=0.11), and significant T2 to T3 decreases in inducible TNF‐α (54% decrease, P=0.007) and IL‐6 (55% decrease, P<0.001). There were no significant changes in either group at the 0.0 ng/mL lipopolysaccharide level for TNF‐α or IL‐6.ConclusionsThe failure to support the primary hypotheses and the unexpected post hoc findings of an exercise‐training–induced proinflammatory response raise questions about whether and under what conditions exercise training has anti‐inflammatory effects.Clinical Trial Registration URL: http://www.clinicaltrials.gov. Unique identifier: NCT01335737.

show abstract

Physical exercise prevents alterations in purinergic system and oxidative status in lipopolysaccharide‐induced sepsis in rats

Miron

Bottari

Assmann

et al. 2018

J of Cellular Biochemistry

View full text Add to dashboard Cite

Sepsis is a generalized infection that involves alterations in inflammatory parameters, oxidant status, and purinergic signaling in many tissues. Physical exercise has emerged as a tool to prevent this disease because of its anti-inflammatory and antioxidant properties. Thus, in this study, we investigated the effects of physical exercise on preventing alterations in purinergic system components, oxidative stress, and inflammatory parameters in lipopolysaccharide (LPS)-induced sepsis in rats. Male Wistar rats were divided into four groups: control, exercise (EX), LPS, and EX+LPS. The resisted physical exercise was performed for 12 weeks on a ladder with 1 m height. After 72 hours of the last exercise session, the animals received 2.5 mg/kg of LPS for induction of sepsis, and after 24 hours, lungs and blood samples were collected for analysis. The results showed that the exercise protocol used was able to prevent, in septic animals: (1) the increase in body temperature; (2) the increase of lipid peroxidation and reactive species levels in the lung, (3) the increase in adenosine triphosphate levels in serum; (4) the change in the activity of the enzymes ectonucleotidases in lymphocytes, partially; (5) the change in the density of purinergic enzymes and receptors in the lung, and (6) the increase of IL-6 and IL-1β gene expression. Our results revealed the involvement of purinergic signaling and oxidative damage in the mechanisms by which exercise prevents sepsis aggravations. Therefore, the regular practice of physical exercise is encouraged as a better way to prepare the body against sepsis complications.

show abstract

Voluntary wheel running attenuates lipopolysaccharide-induced liver inflammation in mice

Cited by 27 publications

References 42 publications

Habitual physical activity protects against lipopolysaccharide-induced inflammation in mouse adipose tissue

Habitual physical activity protects against lipopolysaccharide-induced inflammation in mouse adipose tissue

Aerobic Exercise Training and Inducible Inflammation: Results of a Randomized Controlled Trial in Healthy, Young Adults

Physical exercise prevents alterations in purinergic system and oxidative status in lipopolysaccharide‐induced sepsis in rats

Contact Info

Product

Resources

About