Physical exercise prevents alterations in purinergic system and oxidative status in lipopolysaccharide‐induced sepsis in rats

Abstract

Sepsis is a generalized infection that involves alterations in inflammatory parameters, oxidant status, and purinergic signaling in many tissues. Physical exercise has emerged as a tool to prevent this disease because of its anti-inflammatory and antioxidant properties. Thus, in this study, we investigated the effects of physical exercise on preventing alterations in purinergic system components, oxidative stress, and inflammatory parameters in lipopolysaccharide (LPS)-induced sepsis in rats. Male Wistar rats were divided into four groups: control, exercise (EX), LPS, and EX+LPS. The resisted physical exercise was performed for 12 weeks on a ladder with 1 m height. After 72 hours of the last exercise session, the animals received 2.5 mg/kg of LPS for induction of sepsis, and after 24 hours, lungs and blood samples were collected for analysis. The results showed that the exercise protocol used was able to prevent, in septic animals: (1) the increase in body temperature; (2) the increase of lipid peroxidation and reactive species levels in the lung, (3) the increase in adenosine triphosphate levels in serum; (4) the change in the activity of the enzymes ectonucleotidases in lymphocytes, partially; (5) the change in the density of purinergic enzymes and receptors in the lung, and (6) the increase of IL-6 and IL-1β gene expression. Our results revealed the involvement of purinergic signaling and oxidative damage in the mechanisms by which exercise prevents sepsis aggravations. Therefore, the regular practice of physical exercise is encouraged as a better way to prepare the body against sepsis complications.

Aerobic exercise improves LPS-induced sepsis via regulating the Warburg effect in mice

“…The exercise was known to treat many autoimmune and inflammatory diseases, such as chronic lung diseases and atherosclerosis because AE had immunomodulatory effects and modulated redox balance 8 – 10 . Previous researches demonstrated that LPS injection led to an excessive inflammatory response and oxidative stress injury and LPS injection generally accepted as somewhat modeling the septic condition 11 , 12 . Strikingly, limited research has demonstrated that exercise was a novel tool to prevent sepsis and its complications 11 – 14 .…”

Phytochemical analysis and biological activities of in vitro cultured Nidularium procerum, a bromeliad vulnerable to extinction

“…In the human body, antioxidants are efficient against some metabolic disorders that compromise the corporal homeostasis, such as lipidic and protein peroxidation, DNA degradation and cell membrane alteration 59 . The antioxidant power of N. procerum extracts can be attributed to the molecular structure of compounds, inparticular polyphenolics.…”

Protective effects of aerobic exercise on survival, MOSD, and blood glucose response during sepsis

“…Exercise has emerged as a tool to treat many autoimmune and inflammatory diseases, such as chronic obstructive pulmonary disease (COPD), asthma, and atherosclerosis because exercise has immunomodulatory effects [2][3][4] . However, limited research has demonstrated that exercise has emerged as a novel tool to prevent sepsis and its complications and the beneficial effects of exercise in a septic model have been demonstrated [5][6][7][8][9] . Exercised mice showed a survival benefit compared to unexercised mice in the septic model [8,10,11] .…”