Phytochemical analysis and biological activities of in vitro cultured Nidularium procerum, a bromeliad vulnerable to extinction

Gollo, André Luiz; Tanobe, Valcineide Oliveira de Andrade; Pereira, Gilberto Vinícius de Melo; Marín, Oranys; Bonatto, Sandro José Ribeiro; Silva, Suzany; Barros, Ivan Ricardo de; Soccol, Carlos Ricardo

doi:10.1038/s41598-020-64026-z

Cited by 20 publications

(11 citation statements)

References 88 publications

(63 reference statements)

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“…This study showed that the hydroethanolic extracts of Alstonia boonei and Carica papaya contain alkaloids, flavonoids, phenolics, tannins, saponins, anthraquinones, and terpenoids ( Table 1 ). Alkaloids, flavonoids, and tannins are known for their antioxidant, antimalarial, antimicrobial, anticancer, and anti-inflammatory activities [ 27 , 28 ]. The extracts had total phenolics (34.13 ± 1.90 mg GAE/g for Alstonia boonei and 27.99 ± 1.46 mg GAE/g for Carica papaya ) and total flavonoid (19.47 ± 1.89 mg QE/g for Alstonia boonei and 18.24 ± 1.36 mg QE/g for Carica papaya ).…”

Section: Resultsmentioning

confidence: 99%

“…e extracts had a significant chemosuppressive and curative antimalarial effect on the infected mice when compared with the untreated group. is is believed to be as a result of some of the bioactive compounds such as flavonoids, phenolics, alkaloids, and anthraquinones present in the extracts [27][28][29]. e extracts reduced parasitemia and increased mean survival time in the treated group.…”

Section: Resultsmentioning

confidence: 99%

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Evaluation of Antimalarial Potential of Extracts from Alstonia boonei and Carica papaya in Plasmodium berghei-Infected Mice

Atanu

Idih

Nwonuma

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Extracts of Alstonia boonei and Carica papaya are used in herbal medicine for the treatment of malaria. This work investigated the phytochemical, antioxidant, and antimalarial effects of hydromethanolic extracts of Alstonia boonei and Carica papaya. A four-day chemosuppressive test was conducted to assess the ability of the extracts to prevent establishment of infection. Three doses of the extracts were administered—100, 200, and 400 mg/kg bw—prior to Plasmodium berghei challenge. Change in body weight, parasitemia, packed cell volume (PCV), and mean survival time was determined. A three-day curative test was also carried out on Plasmodium berghei-infected mice to determine the effects of the plant extracts (200 mg/kg bw) on parasitemia and biochemical indices of liver and kidney functions, lipid metabolism, and oxidative stress. The study revealed that the extracts possessed phenolic compounds (34.13 ± 1.90 mg GAE/g for Alstonia boonei and 27.99 ± 1.46 mg GAE/g for Carica papaya) and flavonoids (19.47 ± 1.89 mg QE/g for Alstonia boonei and 18.24 ± 1.36 mg QE/g for Carica papaya). In vitro antioxidant activity measured as total antioxidant power, total reducing power, and DPPH radical scavenging activity showed that the extracts possessed higher antioxidant activity than the reference compounds. The outcome of the chemosuppressive test revealed that whereas Plasmodium berghei-infected mice had high parasitemia, decreased mean survival time, exhibited loss of weight, and had low PCV, treatment with the extracts reversed the effects in a concentration-dependent manner. Similarly, the curative test revealed that the extracts significantly suppressed parasitemia compared with the malaria negative control group. This was mirrored by reversal of indices of hepatic toxicity (AST, ALT, and ALP levels), nephropathy (urea and creatinine levels), oxidative stress (SOD, CAT, GPx, GSH, and lipid peroxides), and dyslipidemia (TC, HDL, and TG levels and HMG-CoA reductase activity) in infected but treated mice compared with negative control. Put together, the results of this study demonstrate that the extracts of Alstonia boonei and Carica papaya possess antimalarial properties and are able to ameliorate metabolic dysregulations that characterize Plasmodium berghei infection. The phytoconstituents in these extracts are believed to be responsible for the pharmacological activity reported in this study.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Evaluation of Antimalarial Potential of Extracts from Alstonia boonei and Carica papaya in Plasmodium berghei-Infected Mice

Atanu

Idih

Nwonuma

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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“…Fenugreek extract @ 25% has proved to be significant for inhibiting fungal radial growth, this could be due to biologically active constituents of T. foenum-graecum (Haouala et al, 2011). The application of fenugreek extract lowered the disease severity under both greenhouse and field conditions, which could be attributed to presence of secondary metabolites i.e., phenolic compounds, flavonoids, tannins and coumarins (Gollo et al, 2020). Fenugreek extract has certain allelochemicals i.e., kaempferol, kaempferol-glucoside and β-d-glucopyranoside (Singh et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Fenugreek extract application improves plant performance of Alternaria solani infected tomato plants

Khan¹

2021

PAKJAS

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Plant extracts (PE’s) has emerged as a safer alternative to manage the fungal pathogens affecting tomato productivity. The current study aimed to evaluate the antimicrobial potential of methanolic fenugreek extract against Alternaria solani, a causal agent of early blight disease in tomato. Fenugreek extract was used at different concentrations of 5%, 10%, 15%, 20% and 25% under in vitro conditions. Results concluded that 25% fenugreek extract significantly reduced the radial growth (2.5 cm) of A. solani under in vitro conditions. Based on in vitro results, three concentrations (5%, 15% and 25%) of fenugreek extract was examined under greenhouse and field conditions. The outcomes expressed that 5% fenugreek extract reduced the disease severity up to 30.19% under greenhouse conditions and up to 40.53% under field trials. Although, application of fenugreek extract had exhibited non-significant impact on vegetative and reproductive growth parameters. However, its application had proved better results as compared to those plants which are infected with A. solani but received zero application of fenugreek extract. Furthermore, the effectiveness of plant extracts was evaluated by variant photosynthetic, antioxidative, polyphenolic and hypersensitive response of A. solani affected tomato plants. The 25% fenugreek extract application had augmented the chlorophyll pigments along with the significant increment of superoxide dismutase (174.16 U mg-1 protein), peroxidase (7.61 µmol min-1 g-1 protein) and catalase activity (4.73 nmol min-1 g-1 protein). Similar outcomes were observed regarding phenolic compounds, where 5% fenugreek extract application had enhanced flavonoid levels (26.62 mg QuE g-1), tannins (1.28 mg TE g-1 extract) and total phenolic contents (2.39 mg GAE g-1) in tomato leaves demonstrating its protective effect against early blight. In dose response, 25% fenugreek extract was most effective in reducing lipid peroxidation and enhancing H2O2 levels. The outcomes of study exhibited the fenugreek extract as an effective strategy to be used against A. solani to control early blight infection in tomato plants. Thus, it can serve as suitable fungicide alternative for resource-poor agriculture areas mainly in developing countries.

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“…Two drops of concentrated HCl (37%, MerckMillipore, Darmstadt, Germany) were added followed by a chip of magnesium metal. Appearance of a magenta color indicated the presence of flavonoids (Akter et al, 2016;Suurbaar et al, 2017;Gollo et al, 2020).…”

Section: Flavonoids Identificationmentioning

confidence: 99%

Nueva información del perfil de compuestos bioactivos, potencial antioxidante y antiproliferativo de Parkinsonia praecox (Fabaceae)

López-Romero¹,

Torres-Moreno²,

Valencia³

et al. 2022

Acta Bot. Mex.

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Antecedentes y Objetivos: Parkinsonia praecox,“palo brea”, es una planta medicinal distribuida en el Desierto de Sonora en México. Sin embargo, existe poca información acerca de la composición química y del potencial biológico de esta planta. Basado en lo anterior, el objetivo del presente estudio fue determinar la composición química y el potencial antioxidante y antiproliferativo de P. praecox. Métodos: Se prepararon extractos metanólicos de tallos (PPS), frutos (PPB) y flores (PPF). La composición química se determinó a través de los métodos de perfil fitoquímico, Folin-Ciocalteu y UPLC-DAD. La actividad antioxidante se evaluó por los métodos DPPH, ABTS, ORAC y FRAP. La actividad antiproliferativa se determinó por el ensayo MTT contra las líneas celulares A549 (cáncer de pulmón de células no pequeñas), MDA-MB-231 (cáncer de mama triple negativo), PC-3 (cáncer de próstata grado IV), HeLa (cáncer de cervix) y L929 (tejido conectivo subcutáneo no canceroso). Resultados: El perfil fitoquímico mostró la presencia de terpenos, compuestos fenólicos, flavonoides, taninos y azúcares en los extractos. PPS presentó la concentración más elevada (p<0.05) de compuestos fenólicos (65.5 mg GAE/g), identificando y cuantificando quercetina (218.86 µg/g). Además, PPS mostró la capacidad más elevada (p<0.05) para estabilizar a los radicales DPPH (IC50: 137 µg/ml), ABTS (39.56 µM TE/g), hidroxilo (ORAC: 1777.78 µM TE/g), y reducir metales (FRAP: 935.6 µM Fe(II)/g). Un comportamiento similar se observó en la actividad antiproliferativa, ya que PPS mostró la citotoxicidad más elevada (p<0.05): A549 (IC50: 341.3 µg/ml), MDA-MB-231 (IC50: 147.3 µg/ml), PC-3 (IC50: 78.8 µg/ml), HeLa (IC50: 121.6 µg/ml) y L929 (IC50: 93.29 µg/ml).Conclusión: Este es el primer estudio donde se reporta el perfil de compuestos bioactivos y el potencial biológico de P. praecox. Los resultados muestran una fuerte asociación entre la actividad antioxidante y antiproliferativa con la presencia de compuestos fenólicos. Esto representa un potencial soporte para el desarrollo de terapias farmacológicas.

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Phytochemical analysis and biological activities of in vitro cultured Nidularium procerum, a bromeliad vulnerable to extinction

Cited by 20 publications

References 88 publications

Evaluation of Antimalarial Potential of Extracts from Alstonia boonei and Carica papaya in Plasmodium berghei-Infected Mice

Evaluation of Antimalarial Potential of Extracts from Alstonia boonei and Carica papaya in Plasmodium berghei-Infected Mice

Fenugreek extract application improves plant performance of Alternaria solani infected tomato plants

Nueva información del perfil de compuestos bioactivos, potencial antioxidante y antiproliferativo de Parkinsonia praecox (Fabaceae)

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