Volume calculation of rats' organs and its application in the validation of the volume relation between the abdominal cavity and the hernial sac in incisional hernias with "loss of abdominal domain"

Araújo, Luz Marina Gonçalves de; Serigiolle, Leonardo Carvalho; Gomes, Helbert Minuncio Pereira; Rodrigues, Daren Athiê Boy; Lopes, Carolina Marques; Leme, Pedro Luiz Squilacci

doi:10.1590/s0102-67202014000300004

Cited by 9 publications

(9 citation statements)

References 22 publications

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“…Tumor sizes were recorded and melanoma lesions, which appear spontaneously at the perianal and ventral tail regions, were mapped. Tumor dimensions were measured with a caliper and tumor volumes calculated by approximating a sphere volume, i.e., using the formula for sphere volume = 4/3 π x radius 1 x radius 2 x radius 3 (mm 3 ) 51 . Deeply infiltrating tumors were rejected for treatment due to possible measurement errors.…”

Section: Methodsmentioning

confidence: 99%

Modulation of stress and immune response by Amblyomin-X results in tumor cell death in a horse melanoma model

Lichtenstein

Iqbal

Will

et al. 2020

Sci Rep

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We have investigated Amblyomin-X-treated horse melanomas to better understand its mode of action through transcriptome analysis and the in vivo model. Amblyomin-X is a Kunitz-type homologous protein that selectively leads to the death of tumor cells via ER stress and apoptosis, currently under investigation as a new drug candidate for cancer treatment. Melanomas are immunogenic tumors, and a better understanding of the immune responses is warranted. Equine melanomas are spontaneous and not so aggressive as human melanomas are, as this study shows that the in vivo treatment of encapsulated horse melanoma tumors led to a significant reduction in the tumor size or even the complete disappearance of the tumor mass through intratumoral injections of Amblyomin-X. Transcriptome analysis identified ER-and mitochondria-stress, modulation of the innate immune system, apoptosis, and possibly immunogenic cell death activation. Interactome analysis showed that Amblyomin-X potentially interacts with key elements found in transcriptomics. Taken together, Amblyomin-X modulated the tumor immune microenvironment in different ways, at least contributing to induce tumor cell death. Melanoma is a type of cancer arising from the malignant transformation of melanocytes, pigment producing-cells found predominantly in the basal layer of the epidermis and eyes. Cutaneous melanoma is the most aggressive and treatment-resistant form of skin cancer responsible for the vast majority of skin cancer-related deaths in the Caucasian population 1. The global incidence of melanoma continues to increase at an alarming rate, despite decades of public prevention programs in many countries. Around 232,000 new cases of skin cancer were recorded worldwide in 2012, accounting for 1.6% of all new cases of cancer back then, while over 300,000 new cases of melanoma were diagnosed worldwide in 2018, according to the World Cancer Research Foundation 2,3. In Brazil, 1,547 deaths were recorded in 2013 due to melanoma, with around 5,690 new cases reported back then, while around 6,260 new cases were expected due in 2018, according to the National Cancer Institute (INCA) 4. Cutaneous melanoma usually affects a higher proportion of patients, in the age range 40-60 years. They can be treated by surgical excision when detected in the early stage (0, I, II and resectable III), however, in the later stages (unresectable III, IV and recurrent melanoma) the treatment options are chemotherapy, target therapy (BRAF/ MEK pathway inhibitors), immunotherapy (checkpoint blockade CTLA-4 receptor inhibition, PD-1 ↔ PD-L1 axis inhibition, and interferon-gamma immunotherapy), or a combination of them. Death in most patients is caused by metastatic disease which may have evolved from the primary tumor. Therefore, there is a need for new

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Section: Methodsmentioning

confidence: 99%

Modulation of stress and immune response by Amblyomin-X results in tumor cell death in a horse melanoma model

Lichtenstein

Iqbal

Will

et al. 2020

Sci Rep

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“…Twenty-five Wistar rats ( Rattus Norvegicus, var. Albinus, Rodentia, Mammalia ), which are genetically similar and present not only isogenic line, but reliable results in studies with small numbers of animals 4 , 24 were randomly distributed in five groups with five animals each; before surgical interventions they were kept in individual cages with access to standardized food and water ad libitum, at 25° C room temperature and a 12 h photoperiod. The animals, with an average weight of 339 g were anesthetized with intraperitoneal injection of ketamine hydrochloride (80 mg/kg) and xylazine (10 mg/kg), being held a median incision with 4 cm in length, opening of the abdominal cavity in linea alba measuring 3.5 cm and a suture in the middle third of each side of the incision was made, with the eversion of the edges of the abdominal rectum muscle without encompassing the peritoneum, thus creating a defect with 3.5x1.5 cm.…”

Section: Methodsmentioning

confidence: 99%

Stem Cells Hematopoietic Niches and Inflammatory Response to Different Synthetic Prosthesis Implanted in Rat With Incisional Hernias

Barbieri

Parreira

Studart

et al. 2017

ABCD, arq. bras. cir. dig.

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Background: Extramedullary hematopoiesis depends on complex pathophysiological mechanisms linked to hematopoietic stem cells and the proteins considered mediators of the inflammation. The identification of hematopoietic cells outside bone marrow in the adult is an occurrence that can occasionally follows the inflammatory response, was considered a secondary occurrence, but current biomolecular studies have changed that concept. Aim: Describe the presence of clusters of precursor cells of platelets (megakaryocytes), and cells of the inflammatory response in the abdominal wall and spleen of rats with experimentally induced incisional hernias and repaired with different synthetic prostheses. Methods: Twenty-five rats with incisional hernias previously performed, were divided into groups of five animals each: Group 1, repair of the hernia defect without prosthetic implant; Group 2, repair with polypropylene prosthesis; Group 3, repair using polypropylene with low weight; Group 4, the use of polypropylene and polyglecaprone prosthesis; Group 5, of polypropylene and polyglactin prosthesis. All prostheses were cut in rhombus format with area 2,625 cm². The animals were reoperated after 10 days, the abdominal walls were removed with the viscera attached to them and the material was processed for histological study. Results: Megakaryocyte niches in the abdominal wall and spleen, occasionally removed together with the adhesions produced in animals with implantation of prostheses and significant inflammatory reaction. Conclusion: The intense inflammatory reaction due to the prostheses with polypropylene in their composition was disproportionate to the expected response, indicating that further studies should be accomplished including immunophenotyping evaluation and specific panels of monoclonal antibodies to better understand the findings.

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“…Samples of kidney, liver, spleen and faeces were also collected from each rat and stored at −20 °C for further analyses. The dimensions of the spleen were recorded for each rat and splenic size was calculated using the formula described by Araujo et al. (2014) in order to investigate any association between Angiostrongylus infection and splenomegaly.…”

Section: Methodsmentioning

confidence: 99%

A survey of Angiostrongylus species in definitive hosts in Queensland

Aghazadeh

Reid

Aland

et al. 2015

International Journal for Parasitology: Parasites and Wildlife

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Despite the recent sporadic reports of angiostrongyliasis in humans, dogs and wildlife in eastern Australia there has been no systematic study to explore the epidemiology of Angiostrongylus spp. in definitive and intermediate hosts in the region. Little is known about the epidemiology of Angiostrongylus species in the definitive host in southeast Queensland, since the only survey conducted in this region was performed in the late 1960s. In this study, free-living populations of Rattus spp. were sampled and examined for the presence of adult and larval Angiostrongylus in the lungs, and of larvae in faeces. The prevalence of infection with Angiostrongylus spp. was 16.5% in Rattus spp. trapped in urban Brisbane and surrounds. This prevalence is much higher than estimates of earlier studies. This highlights the possible risk of zoonotic infection in children, dogs and wildlife in this region and indicates the necessity for public awareness as well as more detailed epidemiological studies on this parasite in eastern Australia.

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Volume calculation of rats' organs and its application in the validation of the volume relation between the abdominal cavity and the hernial sac in incisional hernias with "loss of abdominal domain"

Cited by 9 publications

References 22 publications

Modulation of stress and immune response by Amblyomin-X results in tumor cell death in a horse melanoma model

Modulation of stress and immune response by Amblyomin-X results in tumor cell death in a horse melanoma model

Stem Cells Hematopoietic Niches and Inflammatory Response to Different Synthetic Prosthesis Implanted in Rat With Incisional Hernias

A survey of Angiostrongylus species in definitive hosts in Queensland

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