Voltammetric studies and assay of the anti-inflammatory drug ketoprofen in pharmaceutical formulation and human plasma at a mercury electrode

Abstract

The electrochemical reduction of the anti-inflammatory drug ketoprofen was studied in a Britton-Robinson (B-R.) buffer series of pH 2–11 using dc-polarography, cyclic voltammetry, and coulometry techniques. The electrode reaction pathway of the drug at the dropping mercury electrode was proposed and discussed. A new adsorptive cathodic stripping square-wave voltammetric procedure was optimized for the assay of bulk drug in a B-R. buffer of pH 2.0. The peak current was linear with the drug concentration over the ranges 2 × 10–9 to 2 × 10–7 M of the bulk drug, using a 60 s accumulation time period at –0.6 V (vs. Ag/AgCl/KCls). The percentage recovery of the bulk drug was 99.57 ± 0.54 and a detection limit of 0.10 ng mL–1 was achieved. The proposed procedure was successfully applied for the assay of ketoprofen in pharmaceutical formulation (Ketofan®) and human plasma. The percentage recoveries were 99.66 ± 0.47 and 101.76 ± 0.64 in pharmaceutical formulation and human plasma, respectively. A detection limit of 0.14 ng mL–1 plasma was achieved which was below that reported in literature using the different analytical techniques.Key words: ketoprofen (Ketofan®) determination, polarography, cyclic voltammetry, adsorptive cathodic stripping square-wave voltammetry, human plasma.

Voltammetric/amperometric screening of compounds of pharmacological interest

“…Analgesics (acetaminophen, morfine) C Garcia-Fernandez et al 2000, Ni et al 2004, Uliana et al 2010, Ozcan and Sahin 2011GC Garrido et al 2002, Garrido et al 2003a,b, Torriero et al 2006, Kashefi-Kheyrabadi and Mehrgardi 2012BDD Wangfuengkanagul and Chailapakul 2002, Lourenção et al 2009, Sartori et al 2009, Švorc et al 2012aCNT Wan et al 2009a,b, Goyal et al 2010a,b,c,d,e, Sanghavi and Srivastava 2010,b, Shahrokhian and Asadian 2010, Habibi et al 2011, Lu and Tsai 2011, Arvand and Gholizadeh, 2013 Vela et al 2001, El-Enany et al 2002, Ozaltin et al 2002, Ozaltin et al 2002, Morais et al 2003, Nouws et al 2005a,b, 2006a Belal et al 2001a,b, 2002a, 2001b, 2003, Dumitrescu et al 2001, Ghandour et al 2001, El-Ries et al 2002, Tamer et al 2002, Demircigil et al 2003, Ghoneim and Tawfik 2003a,b, Ioannides et al 2003, Prieto et al 2003, Razak et al 2003 Several general aspects dealing with the voltammetric screening of pharmaceuticals can be applied: -Electrode modification strategies generally used to promote an electrocatalytic effect on the oxidation or reduction of the analyte. In most cases, synergistic eff...…”

Optical Detection of Ketoprofen by Its Electropolymerization on an Indium Tin Oxide-Coated Optical Fiber Probe

“…Electrochemical studies of KP have already been performed using the polarographic method [ 8 , 9 ] or simultaneously cyclic voltammetry and coulometry techniques at the mercury dropping electrode surface [ 8 , 10 ]. Kormosh et al [ 11 ] developed ion-selective electrodes for potentiometric analysis of KP in piroxicam based on Rhodamine 6G in a membrane plasticizer.…”

“…With dropping mercury electrode and using different supporting electrolytes at different pH values it was possible to reach a LOD as low as 5.08 × 10 −4 ng mL −1 . A similar electrode was utilized by Ghoneim and Tawfik [ 10 ], and in a Britton-Robinson buffer (pH 2.0) the LOD was 0.10 ng mL –1 . Next, a setup containing glassy carbon (GC) electrode with multiwalled carbon nanotubes/ionic liquid/chitosan composite for covalent immobilization of the ibuprofen by specific aptamer was proposed [ 7 ].…”

Electrochemical behavior and determination of ketoprofen at glassy--carbon electrode

“…Electrochemical methods have a number of advantages like high selectivity and sensitivity or simple design of the analytical equipment. Electrochemical studies of ketoprofen have already been performed using mercury drop (Ghoneim & Tawfik, 2003), boron-doped diamond (Domínguez, González, Palo, & Sánchez-Martín, 2010) or platinum electrode (Muthu, Latha, G.Bhaskar, & Yoshihara, 2010) and recently indium tin oxide (ITO)-coated optical fiber sensor (Bogdanowicz et al, 2018). The aim of the present work was determine the electrochemical behavior of ketoprofen analyzed at glassy-carbon electrode.…”