“…Different methods were published for the determination of KPN including ion pair chromatography, 5 derivative IR (infrared) spectroscopy, 6 HPLC, 7 electrochemical method, 8 chromatography tandem mass spectrometry 9,10 and terbium sensitized luminescence method. 11 However, most of these methods have some limitations, such as complicated pre-treatment, wasting of time and possible pollution of the environment.…”