Innovative localized surface plasmon resonance sensing technique for a green spectrofluorimetric assay of ketoprofen, paracetamol and chlorzoxazone in pharmaceutical preparations and biological fluids

Abdallah, Nora A.; Fathy, M. E.; Tolba, M. M.; El-Brashy, A.; Ibrahim, F.

doi:10.1039/d2ra04725j

Cited by 8 publications

(3 citation statements)

References 48 publications

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“…Although the studies focused on eliminating the waste and adopting ecofriendly and sustainable methods [26][27][28] started in 1995, they were not assessed by the analytical society. One of the priorities of green analysis is to reduce the use of harmful substances without affecting the efficiency of the methods performance [29][30][31].…”

Section: Evaluation Of Greennessmentioning

confidence: 99%

Green Constant-wavelength Concurrent Selective Fluorescence Method for Assay of Ibuprofen and Chlorzoxazone in Presence of Chlorzoxazone Degradation Product

et al. 2023

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Lower back pain is a universal dilemma leaving a negative effect on both health and life quality. It was found that a fixed dose combination of chlorzoxazone and ibuprofen gave a higher efficiency than analgesic alone in treatment of acute lower back pain. Based on the significant benefit of that combination, a green, sensitive, rapid, direct, and cost-effective method is created for concurrent determination of ibuprofen and chlorzoxazone in presence of 2-amino para chlorophenol (a synthetic precursor and potential impurity of chlorzoxazone) adopting the synchronous spectrofluorimetric technique. Synchronous spectrofluorimetric technique is adopted to avoid the highly overlapped native spectra of both drugs. The synchronous spectrofluorometric method was applied at Δλ = 50 nm, ibuprofen was measured at 227 nm while chlorzoxazone was measured at 282 nm with no hindering from one to another. The various experimental variables affecting the performance of the suggested technique were explored and adjusted. The suggested technique showed good linearity from 0.02 to 0.6 and 0.1 to 5.0 µg/mL for ibuprofen and chlorzoxazone, respectively. The produced detection limits were 0.27 × 10–3 and 0.03, while the quantitation limits were 0.82 × 10–3 and 0.09 µg/mL for ibuprofen and chlorzoxazone, respectively. The suggested approach was successfully applied for the analysis of the studied drugs in the synthetic mixture, different pharmaceutical preparations, and spiked human plasma. The suggested technique was validated with respect to the International Council of Harmonization (ICH) recommendations. The suggested technique was found to be simpler and greener with lower cost compared to the earlier reported methods which required complicated techniques, longer time of analysis, and less safe solvents and reagents. Green profile assessment for the developed method compared with the reported spectrofluorometric method was performed using four assessment tools. These tools confirmed that the recommended technique attained the most possible green parameters, so it could be used as a greener option in routine quality control for analyzing the two drugs in genuine form and pharmaceutical preparations.

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Section: Evaluation Of Greennessmentioning

confidence: 99%

Green Constant-wavelength Concurrent Selective Fluorescence Method for Assay of Ibuprofen and Chlorzoxazone in Presence of Chlorzoxazone Degradation Product

et al. 2023

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“…Green analytical chemistry (GAC) started to attract attention in 2000s [ 4 , 5 ]. This developing field is associated with optimizing the standards of analytical procedures through the decreased usage of hazardous solvents and increased safety for analysts and the planet earth [ 6 , 7 ]. It has recently been preferred not only for pharmaceutical analyses but also for food analyses [ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

Hydro-organic mobile phase and factorial design application to attain green HPLC method for simultaneous assay of paracetamol and dantrolene sodium in combined capsules

et al. 2023

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The greenness of any analytical method has become a very important aspect of a good analytical method. However, most chromatographic methods depend on the usage of relatively large amounts of lethal and un-decaying chemicals and solvents. So, a green approach based on the full factorial design was employed to develop a simple and rapid HPLC technique for concurrent determination of paracetamol and dantrolene sodium in their combined capsules. Both drugs are highly recommended to be administered together in patients with severe musculoskeletal disorders. Avoiding the routine methodology and resorting to the modern technology represented in the usage of experimental design allows rapid determination of the studied drugs using the optimum quantity of chemicals to avoid any waste of resources. Simultaneous separation of a binary mixture of paracetamol and dantrolene sodium was accomplished using a reversed phase Hypersil C18 column using an eco-friendly isocratic eluent. The used mobile phase consisted simply of ethanol: water (40:60, v/v). Orthophosphoric acid was used to adjust the pH of the mobile phase to 4.5. Triethanolamine (0.2%) was added aiming to reduce the peak tailing. The assay was completed within less than 6 min adopting 0.8 mL/min as a flow rate. The detection was carried out using a UV-detector at 290 nm. The suggested technique shows a linear correlation over concentration ranges of 1.0–200 and 1.0–40 µg/mL for paracetamol and dantrolene sodium, respectively. The suggested technique allowed the simultaneous analysis of the two co-formulated drugs in their synthetic mixture and combined capsule. The suggested technique is considered a greener substitute for the other reported HPLC techniques through the usage of safer solvents and chemicals, along with decreasing both waste output and analysis time. The method is accurate with recoveries between 97.85 and 101.27%, precise, as %RSD for the intraday and interday precision were between 0.39 and 1.72% and very sensitive with limits of detection (LOD)’s 0.15 and 0.18 µg/ml and limits of quantification (LOQ)’s 0.48 and 0.61 µg/ml for paracetamol and dantrolene sodium, respectively. The method greenness was ensured through its assessment by four greenness metrics. It is also validated following the International Conference on Harmonization Guidelines. The recommended technique could be a good alternative to traditional methods in the routine quality control analysis of the studied drugs due to its minimum harm to the planet or human beings.

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“…Also, the spectrofluorimetric method at ultra-trace levels where an aqueous mixture of sulfuric acid was used. The method was inappropriate due to this reagent which was a controlled narcotic [9,10]. Finally, radiometric fluorescent sensors for the rapid detection of AC where hydrochloric acid was used during the experimental methodology were also used [11].…”

Section: Introductionmentioning

confidence: 99%

A Facile Glycerol-Assisted Synthesis of Low-Cu2+-Doped CoFe2O4 for Electrochemical Sensing of Acetaminophen

Alfonso-González,

Granja-Banguera,

Morales-Morales

et al. 2023

Biosensors

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This work devised a simple glycerol-assisted synthesis of a low-Cu2+-doped CoFe2O4 and the electrochemical detection of acetaminophen (AC). During the synthesis, several polyalcohols were tested, indicating the efficiency of glycerin as a cosolvent, aiding in the creation of electrode-modifier nanomaterials. A duration of standing time (eight hours) before calcination produces a decrease in the secondary phase of hematite. The synthesized material was used as an electrode material in the detection of AC. In acidic conditions (pH 2.5), the limit of detection (LOD) was 99.4 nM, while the limit of quantification (LOQ) was found to be (331 nM). The relative standard deviation (RSD), 3.31%, was computed. The enhanced electrocatalytic activity of a low-Cu2+-doped CoFe2O4-modified electrode Cu0.13Co0.87Fe2O4/GCE corresponds extremely well with its resistance Rct, which was determined using the electrochemical impedance spectroscopy (EIS) technique and defined its electron transfer capacity. The possibility of a low-Cu2+-doped CoFe2O4 for the electrochemical sensing of AC in human urine samples was studied. The recovery rates ranging from 96.5 to 101.0% were obtained. These findings suggested that the Cu0.13Co0.87Fe2O4/GCE sensor has outstanding practicability and could be utilized to detect AC content in real complex biological samples.

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Innovative localized surface plasmon resonance sensing technique for a green spectrofluorimetric assay of ketoprofen, paracetamol and chlorzoxazone in pharmaceutical preparations and biological fluids

Abstract: A green, quick and sensitive spectrofluorimetric technique was investigated and validated for the assay of three different drugs namely, ketoprofen (KPN), paracetamol (PAR), and chlorzoxazone (CLX).

Cited by 8 publications

References 48 publications

Green Constant-wavelength Concurrent Selective Fluorescence Method for Assay of Ibuprofen and Chlorzoxazone in Presence of Chlorzoxazone Degradation Product

Green Constant-wavelength Concurrent Selective Fluorescence Method for Assay of Ibuprofen and Chlorzoxazone in Presence of Chlorzoxazone Degradation Product

Hydro-organic mobile phase and factorial design application to attain green HPLC method for simultaneous assay of paracetamol and dantrolene sodium in combined capsules

A Facile Glycerol-Assisted Synthesis of Low-Cu2+-Doped CoFe2O4 for Electrochemical Sensing of Acetaminophen

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