Voltammetric determination of electron-transfer rate between an enzyme and a mediator

Rusling, James F.; Itô, Kazunori

doi:10.1016/0003-2670(91)87192-a

Cited by 28 publications

(18 citation statements)

References 10 publications

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“…Our results completely agree with the conclusions drawn by Rusling for the oxidation of glucose oxidase by ferrocenecarboxylic acid [33]. He noted a two fold overestimation of the rate constant when the simple EC H model is used neglecting the twoelectron nature of the oxidation.…”

Section: Other Data Treatment Approachessupporting

confidence: 93%

Electrocatalytic Oxidation of Dihydronicotinamide Adenine Dinucleotide with Ferrocene Carboxylic Acid by Diaphorase fromClostridium kluveri. Remarks on the Kinetic Approaches Usually Adopted

Antiochia

Lavagnini

Magno³

1999

Electroanalysis

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The kinetics of the electron transfer between NADH and ferrocenecarboxylic acid (FMCA) catalyzed by diaphorase from Clostridium kluveri (EC 1.8.1.4.) was studied by voltammetry. A commercially available digital simulation program permitted one to determine the rate constants of the catalytic system by a ®tting procedure. A rate constant of 3610 4 M À1 s À1 was found for the reaction between diaphorase and mediator while a value of 8 M À1 s À1 was found for the parallel competitive reaction between NADH and mediator. In addition a comparison of the present method to other data treatment procedures reported in the literature and a brief evaluation of their limits of applicability were carried out.

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Section: Other Data Treatment Approachessupporting

confidence: 93%

Electrocatalytic Oxidation of Dihydronicotinamide Adenine Dinucleotide with Ferrocene Carboxylic Acid by Diaphorase fromClostridium kluveri. Remarks on the Kinetic Approaches Usually Adopted

Antiochia

Lavagnini

Magno³

1999

Electroanalysis

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“…Research in the development of enzyme electrodes for biofuel cell and biosensor applications has been carried out extensively in recent years. Studies on understanding the reaction mechanisms of enzyme catalytic reactions [14,15], developing new biomaterials [16][17][18][19][20], on enzyme modification [21][22][23][24][25][26][27], enzyme immobilisation methods [28][29][30][31][32][33][34], and enzyme electrode structures [35] have been reported in the literature in an effort to improve the performance of enzyme electrodes.…”

Section: Open Accessmentioning

confidence: 99%

Enzymatic Biofuel Cells—Fabrication of Enzyme Electrodes

Scott

2010

Energies

126

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Enzyme based bioelectronics have attracted increasing interest in recent years because of their applications on biomedical research and healthcare. They also have broad applications in environmental monitoring, and as the power source for portable electronic devices. In this review, the technology developed for fabrication of enzyme electrodes has been described. Different enzyme immobilisation methods using layered structures with self-assembled monolayers (SAM) and entrapment of enzymes in polymer matrixes have been reviewed. The performances of enzymatic biofuel cells are summarised. Various approaches on further development to overcome the current challenges have been discussed. This innovative technology will have a major impact and benefit medical science and clinical research, healthcare management, energy production from renewable sources.

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“…Several researchers have reported simulations of mediated enzyme electrochemistry [2,3,4,5,6,7,8,9,10,11,12,13,14,15]. Bartlett and Pratt have reviewed previous works on the simulation of enzyme electrodes [16].…”

Section: Introductionmentioning

confidence: 99%

“…On the other hand, digital simulation to obtain a numerical solution can be applied to any case, and neither simplification nor classification is necessary. Many digital simulations for steady-state and transient responses of enzyme electrochemistry have been reported [2,3,4,7,8,10,11,12]. However, concentration polarization of the substrate in the vicinity of an electrode surface has never been considered for cyclic voltammetric simulation of an enzyme mediated reaction.…”

Section: Introductionmentioning

confidence: 99%

Cyclic voltammetric simulation of electrochemically mediated enzyme reaction and elucidation of biosensor behaviors

Yokoyama

Koide

Kayanuma

2002

Analytical and Bioanalytical Chemistry

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A cyclic voltammetric simulation that can be applied to an electrochemically mediated enzyme reaction involving any substrate and mediator concentration was developed. Concentration polarization of the substrate in the vicinity of an electrode was considered as well as mediator concentration. Reversible electrochemical reaction with one electron followed by an enzyme reaction with two electrons was modeled. The differential equations for the mediator and substrate were solved using digital simulation techniques. The calculated cyclic voltammograms showed prepeaks when there was a low substrate concentration, high mediator concentration, and high enzyme activity. The prepeak was experimentally observed in the case of an enzyme electrode co-immobilized with a redox polymer. The enzyme electrode loaded at high redox polymer and high enzyme content showed a prepeak at low substrate concentration in the cyclic voltammogram.

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Voltammetric determination of electron-transfer rate between an enzyme and a mediator

Cited by 28 publications

References 10 publications

Electrocatalytic Oxidation of Dihydronicotinamide Adenine Dinucleotide with Ferrocene Carboxylic Acid by Diaphorase fromClostridium kluveri. Remarks on the Kinetic Approaches Usually Adopted

Electrocatalytic Oxidation of Dihydronicotinamide Adenine Dinucleotide with Ferrocene Carboxylic Acid by Diaphorase fromClostridium kluveri. Remarks on the Kinetic Approaches Usually Adopted

Enzymatic Biofuel Cells—Fabrication of Enzyme Electrodes

Cyclic voltammetric simulation of electrochemically mediated enzyme reaction and elucidation of biosensor behaviors

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