Cyclic voltammetric simulation of electrochemically mediated enzyme reaction and elucidation of biosensor behaviors

Abstract

A cyclic voltammetric simulation that can be applied to an electrochemically mediated enzyme reaction involving any substrate and mediator concentration was developed. Concentration polarization of the substrate in the vicinity of an electrode was considered as well as mediator concentration. Reversible electrochemical reaction with one electron followed by an enzyme reaction with two electrons was modeled. The differential equations for the mediator and substrate were solved using digital simulation techniques. The calculated cyclic voltammograms showed prepeaks when there was a low substrate concentration, high mediator concentration, and high enzyme activity. The prepeak was experimentally observed in the case of an enzyme electrode co-immobilized with a redox polymer. The enzyme electrode loaded at high redox polymer and high enzyme content showed a prepeak at low substrate concentration in the cyclic voltammogram.

Long‐Distance Electron Transfer by G. sulfurreducens Biofilms Results in Accumulation of Reduced c‐Type Cytochromes

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Utilization of AC Impedance Measurements for Electrochemical Glucose Sensing Using Glucose Oxidase to Improve Detection Selectivity

“…Numerical calculations involving electrochemical and enzymatic reactions with diffusion of the electron mediators were conducted by calculating the mass balance in one-dimensional discrete cells according to the finite difference method reported by Yokoyama et al 35,36 All calculations were conducted by using homemade software running on an IBM compatible PC programmed with Microsoft Visual Basic.NET.…”

Mechanistic modeling of cyclic voltammetry: A helpful tool for understanding biosensor principles and supporting design optimization

“…The presented model allowed determining the kinetic constants for the homogeneous reaction between glucose oxidase (GOx) and glucose in the presence of ferrocene derivatives. Later on, the model principles were applied for modeling the reversible electrochemical reaction with one electron followed by the mediated reaction of immobilized enzyme with two electrons [18]. The simulation of cyclic voltammetry was also applied for studying the influence of ohmic drop [19,20], electrode area, electrochemical reaction constants and transferring coefficients [21], as well as the effect of flow rates [22][23][24] on the current response and wave shape.…”