Long‐Distance Electron Transfer by <i>G. sulfurreducens</i> Biofilms Results in Accumulation of Reduced <i>c</i>‐Type Cytochromes

Liu, Ying; Bond, Daniel R.

doi:10.1002/cssc.201100734

Cited by 114 publications

(103 citation statements)

References 36 publications

(67 reference statements)

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“…The redox peaks at formal potential of -0.372 V and -0.289 V resulted from the electrochemically active molecules in biofilm which also were obtained in previous studies (Liu et al 2005;Liu and Bond 2012a;Liu et al 2010a;Liu et al 2008a). …”

Section: Dependence Of Current On Sensed Frequency For Mature Biofilmsupporting

confidence: 83%

“…While current is recorded at a constant potential from many layers of biofilm on the electrode surface. From our previous studies (Liu and Bond 2012b), most cells in biofilm will contribute to the current production although most outer layers may have lower electron transfer efficiency than cells in the deep layers. When the substrate was exhausted completely in the first batch, another 15 mM acetate was added in the bioreactor and during chronoamperometry running the frequency was recorded simultaneously at this second batch.…”

Section: Characterization Of Sensed Frequency and Current Producion Fmentioning

confidence: 95%

“…In this respect, the interface between G. sulfurreducens cells and electrodes was studied using a spectro-electrochemical approach by attenuated total reflection-SEIRAS (ATR-SEIRAS) (Busalmen et al 2008). Most importantly, Bond group advanced a nondestructive in situ linking technology of electrochemical and UV-visible spectroscopy through which the function of redox cytochrome c in biofilm in situ was successfully revealed (Liu and Bond 2012a;Liu et al 2011). These studies strongly confirmed that outer-membrane cytochrome c molecules were responsible of the electron transfer processes of biofilms attached on electrodes.…”

Section: Introductionmentioning

confidence: 99%

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Real-time monitoring of electrochemically active biofilm developing behavior on bioanode by using EQCM and ATR/FTIR

Liu

Berná

Climent

et al. 2015

Sensors and Actuators B: Chemical

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It is crucial to study the bacterial attaching behavior for revealing information of electrochemically active biofilm on the interface of liquid/electrode in microbial fuel cells (MFCs). In the current study, mixed-culture from waste water as model bacteria was investigated for gaining more sight onto relationship between current and biomass or onto bioadhesion mechanism at the molecular level using Electrochemical Quartz Crystal Microbalance (EQCM) and Attenuated Total Reflection-SEIRAS (ATR-SEIRAS) combined with electrochemistry. From the frequency shift using Sauerbrey equation via EQCM the maximum cells mass of 11.5 μg/cm 2 was estimated for the mature biofilm. Notably, the highest current density of 110 μA/μg·cm 2 occurred before maximum biomass coming, which indicated that mature biofilm may not be an optimal state for enhancing power output of MFCs. Furthermore, the sensed biomass attached on electrode for mature biofilm will be constant for more than 40 h * Corresponding author, On the other hand, using ATR-SEIRAS techniques the obvious adsorbed water structure change during biofilm formation on electrode surface was observed and showed that the absorption bands linked to bacterial adsorption increased. It can be concluded that water adsorption accompanies the adsorbed bacteria and the cells number attaching on the electrode increased with time. Especially, the direct contact of bacteria and electrode via outer-membrane protein can be confirmed via series spectra at amideⅠand amideⅡ modes and water movement from negative bands displacing by adsorbed bacteria. Our study provided supplementary information about the interaction between the microbes and solid electron acceptors beyond traditional electrochemistry. It is expected to explore more information on electrochemically active microbial kinetics for improvement of their competence and understanding of attaching mechanisms in nature using our approach.

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Section: Dependence Of Current On Sensed Frequency For Mature Biofilmsupporting

confidence: 83%

Section: Characterization Of Sensed Frequency and Current Producion Fmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Real-time monitoring of electrochemically active biofilm developing behavior on bioanode by using EQCM and ATR/FTIR

Liu

Berná

Climent

et al. 2015

Sensors and Actuators B: Chemical

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“…The interaction between microorganisms and electrical conductive structures makes these systems unique and versatile, with potential applications in electrical energy storage,4, 5 energy‐ and nutrient‐recovering wastewater treatment systems,6, 7, 8 production of high‐value chemical commodities,9, 10 long‐term off‐grid low‐power electricity generation,11, 12 and the development of highly specific and innovative biosensors 13, 14. Additionally, microorganisms may provide temporal charge storage within the microbial cell 15, 16, 17. From a scientific perspective, BESs may function as a platform for fundamental microbiological studies,18, 19, 20 as unique metabolic properties21 can now be studied by using a plethora of electrochemical analyses 22, 23, 24…”

Section: Introductionmentioning

confidence: 99%

In situ Biofilm Quantification in Bioelectrochemical Systems by using Optical Coherence Tomography

Molenaar

Sleutels²,

Pereirã³

et al. 2018

ChemSusChem

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Detailed studies of microbial growth in bioelectrochemical systems (BESs) are required for their suitable design and operation. Here, we report the use of optical coherence tomography (OCT) as a tool for in situ and noninvasive quantification of biofilm growth on electrodes (bioanodes). An experimental platform is designed and described in which transparent electrodes are used to allow real‐time, 3D biofilm imaging. The accuracy and precision of the developed method is assessed by relating the OCT results to well‐established standards for biofilm quantification (chemical oxygen demand (COD) and total N content) and show high correspondence to these standards. Biofilm thickness observed by OCT ranged between 3 and 90 μm for experimental durations ranging from 1 to 24 days. This translated to growth yields between 38 and 42 mgCODbiomass gCODacetate −1 at an anode potential of −0.35 V versus Ag/AgCl. Time‐lapse observations of an experimental run performed in duplicate show high reproducibility in obtained microbial growth yield by the developed method. As such, we identify OCT as a powerful tool for conducting in‐depth characterizations of microbial growth dynamics in BESs. Additionally, the presented platform allows concomitant application of this method with various optical and electrochemical techniques.

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“…This leads to the progressive accumulation of reduced c‐Cyts in the biofilm matrix (Liu and Bond, 2012) and the generation of a redox gradient that provides the driving force for electrons to flow towards the underlying electrode (Snider et al ., 2012). To alleviate the electron–acceptor limitation imposed by the c‐Cyts, cells in this upper stratum use the conductive T4P network as the primary path for electron discharges (Steidl et al ., 2016).…”

Section: Synthetic Biology Goes Electronicmentioning

confidence: 99%

Harnessing the power of microbial nanowires

Reguera

2018

Microbial Biotechnology

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SummaryThe reduction of iron oxide minerals and uranium in model metal reducers in the genus Geobacter is mediated by conductive pili composed primarily of a structurally divergent pilin peptide that is otherwise recognized, processed and assembled in the inner membrane by a conserved Type IVa pilus apparatus. Electronic coupling among the peptides is promoted upon assembly, allowing the discharge of respiratory electrons at rates that greatly exceed the rates of cellular respiration. Harnessing the unique properties of these conductive appendages and their peptide building blocks in metal bioremediation will require understanding of how the pilins assemble to form a protein nanowire with specialized sites for metal immobilization. Also important are insights into how cells assemble the pili to make an electroactive matrix and grow on electrodes as biofilms that harvest electrical currents from the oxidation of waste organic substrates. Genetic engineering shows promise to modulate the properties of the peptide building blocks, protein nanowires and current‐harvesting biofilms for various applications. This minireview discusses what is known about the pilus material properties and reactions they catalyse and how this information can be harnessed in nanotechnology, bioremediation and bioenergy applications.

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Long‐Distance Electron Transfer by G. sulfurreducens Biofilms Results in Accumulation of Reduced c‐Type Cytochromes

Cited by 114 publications

References 36 publications

Real-time monitoring of electrochemically active biofilm developing behavior on bioanode by using EQCM and ATR/FTIR

Real-time monitoring of electrochemically active biofilm developing behavior on bioanode by using EQCM and ATR/FTIR

In situ Biofilm Quantification in Bioelectrochemical Systems by using Optical Coherence Tomography

Harnessing the power of microbial nanowires

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