A crucial aspect for the application of bioelectrochemical systems (BESs) as a wastewater treatment technology is the efficient oxidation of complex substrates by the bioanode, which is reflected in high Coulombic efficiency (CE). To achieve high CE, it is essential to give a competitive advantage to electrogens over methanogens. Factors that affect CE in bioanodes are, amongst others, the type of wastewater, anode potential, substrate concentration and pH. In this paper, we focus on acetate as a substrate and analyze the competition between methanogens and electrogens from a thermodynamic and kinetic point of view. We reviewed experimental data from earlier studies and propose that low substrate loading in combination with a sufficiently high anode overpotential plays a key-role in achieving high CE. Low substrate loading is a proven strategy against methanogenic activity in large-scale reactors for sulfate reduction. The combination of low substrate loading with sufficiently high overpotential is essential because it results in favorable growth kinetics of electrogens compared to methanogens. To achieve high current density in combination with low substrate concentrations, it is essential to have a high specific anode surface area. New reactor designs with these features are essential for BESs to be successful in wastewater treatment in the future.
Detailed studies of microbial growth in bioelectrochemical systems (BESs) are required for their suitable design and operation. Here, we report the use of optical coherence tomography (OCT) as a tool for in situ and noninvasive quantification of biofilm growth on electrodes (bioanodes). An experimental platform is designed and described in which transparent electrodes are used to allow real‐time, 3D biofilm imaging. The accuracy and precision of the developed method is assessed by relating the OCT results to well‐established standards for biofilm quantification (chemical oxygen demand (COD) and total N content) and show high correspondence to these standards. Biofilm thickness observed by OCT ranged between 3 and 90 μm for experimental durations ranging from 1 to 24 days. This translated to growth yields between 38 and 42 mgCODbiomass gCODacetate −1 at an anode potential of −0.35 V versus Ag/AgCl. Time‐lapse observations of an experimental run performed in duplicate show high reproducibility in obtained microbial growth yield by the developed method. As such, we identify OCT as a powerful tool for conducting in‐depth characterizations of microbial growth dynamics in BESs. Additionally, the presented platform allows concomitant application of this method with various optical and electrochemical techniques.
Bioelectrochemical systems hold potential for both conversion of electricity into chemicals through microbial electrosynthesis (MES) and the provision of electrical power by oxidation of organics using microbial fuel cells (MFCs). This study provides a proof of concept for a microbial rechargeable battery (MRB) allowing storage of electricity by combining MES and a MFC in one system. Hexacyanoferrate(II/III) was used as counter redox couple. Duplicate runs showed stable performance over 15 days, with acetate being the main energy carrier. An energy density of around 0.1 kWh/m 3 (normalized to anode electrolyte volume) was achieved at a full cycle energy efficiency of 30−40%, with a nominal power output during discharge of 190 W/m 3 (normalized to anode volume). With this study, we show a new potential application area for bioelectrochemical systems as a future local energy storage device.
Microbial electrosynthesis is a useful form of technology for the renewable production of organic commodities from biologically catalyzed reduction of CO2. However, for the technology to become applicable, process selectivity, stability and efficiency need strong improvement. Here we report on the effect of different electrochemical control modes (potentiostatic/galvanostatic) on both the start-up characteristics and steady-state performance of biocathodes using a non-enriched mixed-culture inoculum. Based on our results, it seems that kinetic differences exist between the two dominant functional microbial groups (i.e., homoacetogens and methanogens) and that by applying different current densities, these differences may be exploited to steer product selectivity and reactor performance.
Recently, the microbial rechargeable battery (MRB) has been proposed as a potentially sustainable and low-cost electrical energy storage technology. In the MRB, bioelectrochemical CO 2 reduction and subsequent product oxidation has successfully been combined in one integrated system. However, finding a suitable counter electrode is hindering its further development. In this work, we have tested two alternative counter electrodes in duplicate-namely, i) oxygen/water and ii) a capacitive electrode-for use in the MRB platform. During daily charge/ discharge cycling over periods of 11 to 15 days, experimentally obtained energy efficiencies of 25 and 3.7 % were reported when using the capacitive and the oxygen/water electrodes, respectively. Large overpotentials, resulting in a voltage efficiency of 15 % and oxygen crossover leading to coulombic efficiencies of 25 % caused the considerably lower efficiency for the oxygen/water systems, despite the theoretical higher voltage efficiency. Although the capacitive electrode equipped systems performed better, energy density is limited by the operational potential window within which capacitive systems can operate reliably. Microbial community analysis revealed dominant presence of Geobacter in the bioanode and Selenomonadales in the biocathode. These results do not necessarily bring practical application of the MRB closer, but they do provide new insights in the working principle of this new technology.[a] Dr.
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