NP-based enzymatic biosensors were prepared by the simultaneous encapsulation of glucose and alcohol oxidases, Nafion and noble metal NPs via co-deposition from a phosphate multiple electrolyte on top of the sensor surface.

Highlights  The physicochemical parameters required for mathematical modeling were either found in literature or estimated on the basis of experimental data. Thus, the applicability of the developed model is not limited by the dataset or biosensor design.  The developed cyclic voltammetry simulator was applied for interpreting the experimental results at various mediator concentrations, membrane thickness/compositions and operating conditions of the proposed multi-layer biosensor system.  An accurate electrochemical, morphological and microscopic characterization of the biosensor system coupled with the model predictions allowed to identify the parameters crucial for the stable biosensor response.  Based on the model predictions, a more favourable design of the biosensor system was developed, which subsequently reduced the reagent usage and waste generation.

Organic-inorganic hybrids (OIH) are considered to be a powerful platform for applications in many research and industrial fields. This review highlights the application of OIH for chemical analysis, biosensors, and environmental monitoring. A methodology toward metrological traceability measurement and standardization of OIH and demonstration of the role of mathematical modeling in biosensor design are also presented. The importance of the development of novel types of OIH for biosensing applications is highlighted. Finally, current trends in nanometrology and nanobiosensors are presented.

Development of a tandem monitoring approach that allows the simultaneous on-line detection of multiple biosensor system parameters.

Herein, we introduce an original strategy towards one-step encapsulation, storage and controlled release of low molecular weight organic compounds via electroplated nanoparticles.