Abstract:NP-based enzymatic biosensors were prepared by the simultaneous encapsulation of glucose and alcohol oxidases, Nafion and noble metal NPs via co-deposition from a phosphate multiple electrolyte on top of the sensor surface.
“…In order to solve the problems associated with the LbL preparation method, recently a novel one-step technique was proposed for the production of tailored enzymatic nanobiosensors that are instrumentally controlled and allow reproducible, spatial, and temporal resolution, simultaneous multi-analyte detection with high specificity [67]. However, it should be noted that regardless of the fabrication method, the general guidelines towards validation of the OIH in general, as well as towards standardization of biosensors and nanobiosensors as a case study are still under development (see section below titled "The role of nanoanalytics and nanometrology in OIH development").…”
Section: Biosensors As a Targeted Class Of Oihmentioning
Organic-inorganic hybrids (OIH) are considered to be a powerful platform for applications in many research and industrial fields. This review highlights the application of OIH for chemical analysis, biosensors, and environmental monitoring. A methodology toward metrological traceability measurement and standardization of OIH and demonstration of the role of mathematical modeling in biosensor design are also presented. The importance of the development of novel types of OIH for biosensing applications is highlighted. Finally, current trends in nanometrology and nanobiosensors are presented.
“…In order to solve the problems associated with the LbL preparation method, recently a novel one-step technique was proposed for the production of tailored enzymatic nanobiosensors that are instrumentally controlled and allow reproducible, spatial, and temporal resolution, simultaneous multi-analyte detection with high specificity [67]. However, it should be noted that regardless of the fabrication method, the general guidelines towards validation of the OIH in general, as well as towards standardization of biosensors and nanobiosensors as a case study are still under development (see section below titled "The role of nanoanalytics and nanometrology in OIH development").…”
Section: Biosensors As a Targeted Class Of Oihmentioning
Organic-inorganic hybrids (OIH) are considered to be a powerful platform for applications in many research and industrial fields. This review highlights the application of OIH for chemical analysis, biosensors, and environmental monitoring. A methodology toward metrological traceability measurement and standardization of OIH and demonstration of the role of mathematical modeling in biosensor design are also presented. The importance of the development of novel types of OIH for biosensing applications is highlighted. Finally, current trends in nanometrology and nanobiosensors are presented.
“…On the basis of bioelectrocatalysis, amperometric biosensors are analytical devices used to detect specific target analytes (substrates) and have been widely used in various fields such as medical care, environmental monitoring, food safety and industrial bioprocess monitoring [ 12 , 13 , 32 , 33 ]. Generally, there are many compounds (analytes) in the target fluid.…”
Section: Introductionmentioning
confidence: 99%
“…However, the response of an amperometric sensor depends on detection time, which limits practical application [ 4 , 9 , 11 , 12 , 13 , 26 , 32 , 33 ]. It is necessary for amperometric sensors to obtain steady-state currents.…”
Bioelectrocatalysis provides the intrinsic catalytic functions of redox enzymes to nonspecific electrode reactions and is the most important and basic concept for electrochemical biosensors. This review starts by describing fundamental characteristics of bioelectrocatalytic reactions in mediated and direct electron transfer types from a theoretical viewpoint and summarizes amperometric biosensors based on multi-enzymatic cascades and for multianalyte detection. The review also introduces prospective aspects of two new concepts of biosensors: mass-transfer-controlled (pseudo)steady-state amperometry at microelectrodes with enhanced enzymatic activity without calibration curves and potentiometric coulometry at enzyme/mediator-immobilized biosensors for absolute determination.
“…Amperometric biosensors on the basis of bioelectrocatalysis are analytical devices to detect specific target analytes (substrates) and have been widely used in various fields such as medical care, environmental monitoring, food safety, and industrial bioprocess monitoring [12,13,32,33]. Generally, there are many compounds (analytes) in the target fluid.…”
Section: Introductionmentioning
confidence: 99%
“…The response of an amperometric sensor, however, depends on the detection time, and it limits the practical application [4,9,[11][12][13]26,32,33]. It is necessary for amperometric sensors to obtain steady-state currents.…”
Bioelectrocatalysis provides the intrinsic catalytic-functions of redox enzymes to non-specific electrode reactions and is the most important and basic concept for biosensors. This review starts by describing fundamental characteristics of bioelectrocatalytic reactions in mediated and direct electron transfer types from a theoretical viewpoint and summarizes amperometric biosensors based on multi-enzymatic cascades and for multi-analyte detection. The review also introduces prospective aspects of two new concepts of biosensors: mass-transfer-controlled (pseudo)steady-state amperometry at microelectrodes with enhanced enzymatic activity without calibration curves and potentiometric coulometry at enzyme/mediator-immobilized biosensors for absolute determination.
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