Voltage‐Sensitive and Ligand‐Gated Channels in Differentiating Neural Stem–Like Cells Derived from the Nonhematopoietic Fraction of Human Umbilical Cord Blood

Sun, Wei; Bużañska, Leonora; Domańska-Janik, Krystyna; Salvi, Richard; Stachowiak, Michal K.

doi:10.1634/stemcells.2004-0316

Cited by 90 publications

(54 citation statements)

References 81 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…6 These features are attractive to investigators working to develop cell-based therapies to treat human diseases. Upon exposure to specific growth factors, 7 MSCs are able to differentiate to neuron-like cells, and thus have potential applications in the treatment of neurological diseases. 8 In addition to the direct transdifferentiation into neural cells, MSCs can perform regenerative functions in neural tissues through a number of mechanisms including secretion of trophic factors, inhibition of apoptosis, stimulation of endogenous stem cells and immunomodulation resulting in decreased inflammation.…”

Section: Introductionmentioning

confidence: 99%

Viability and neural differentiation of mesenchymal stem cells derived from the umbilical cord following perinatal asphyxia

et al. 2011

View full text Add to dashboard Cite

Objective: Hypoxia-ischemia is the leading cause of neurological handicaps in newborns worldwide. Mesenchymal stem cells (MSCs) collected from fresh cord blood of asphyxiated newborns have the potential to regenerate damaged neural tissues. The aim of this study was to examine the capacity for MSCs to differentiate into neural tissue that could subsequently be used for autologous transplantation.Study Design: We collected cord blood samples from full-term newborns with perinatal hypoxemia (n ¼ 27), healthy newborns (n ¼ 14) and non-hypoxic premature neonates (n ¼ 14). Mononuclear cells were separated, counted, and then analyzed by flow cytometry to assess various stem cell populations. MSCs were isolated by plastic adherence and characterized by morphology. Cells underwent immunophenotyping and trilineage differentiation potential. They were then cultured in conditions favoring neural differentiation. Neural lineage commitment was detected using immunohistochemical staining for glial fibrillary acidic protein, tubulin III and oligodendrocyte marker O4 antibodies.Result: Mononuclear cell count and viability did not differ among the three groups of infants. Neural differentiation was best demonstrated in the cells derived from hypoxia-ischemia term neonates, of which 69% had complete and 31% had partial neural differentiation. Cells derived from preterm neonates had the least amount of neural differentiation, whereas partial differentiation was observed in only 12%.Conclusion: These findings support the potential utilization of umbilical cord stem cells as a source for autologous transplant in asphyxiated neonates.

show abstract

Section: Introductionmentioning

confidence: 99%

Viability and neural differentiation of mesenchymal stem cells derived from the umbilical cord following perinatal asphyxia

et al. 2011

View full text Add to dashboard Cite

show abstract

“…these include stem/progenitor cells derived (i) from human umbilical cord blood stem cells, (ii) directly from human fetal brain tissue, or (iii) from human embryonic stem cells (eSC). the earliest work on such systems included (i) a neural stem cell line generated from human umbilical cord blood stem cells (HUCB-NSC line; Buzanska et al, 2005) and (ii) human neural progenitor cells (NPCs) growing as neurospheres (Fritsche et al, 2005). the difference in time of origin of these cells (embryonic vs. fetal) is complementary, covering the embryonic and early fetal period of neurodevelopment, respectively.…”

Section: Clinical Perspectives Of Developmental Neurotoxicitymentioning

confidence: 99%

“…Some of the major hurdles associated with in vitro screening are minimized when testing with an intact organism. Because the zebrafish liver mahave been further developed and characterized, especially for their DNt testing applicability ((i): Sun et al, 2005;Jurga et al, 2006;Buzanska et al, 2009;Zychowicz et al, 2011;(ii) : Moors et al, 2007: Moors et al, , 2009Schreiber et al, 2010;Gassmann et al, 2010;tegenge et al, 2010;Fritsche et al, 2011). Human embryonic stem cells were recently differentiated in vitro for the purpose of DNt testing.…”

Section: Potential Of Non-mammalian Species As Relevant Dnt Modelsmentioning

confidence: 99%

“…For chemical effects on neural differentiation, migration, synaptogenesis and functional network activity, less data is available. Differentiation of HUCB-NSC, NPC, or rat CGC into neurons and/or oligodendrocytes have also been assessed using more limited numbers of chemicals (Buzanska et al, 2005;Hogberg et al, 2009;Fritsche et al, 2005;Moors et al, 2009, Schreiber et al, 2010. Not only the ability to differentiate, but also to reach the correct position by cell migration can be studied, preferably in spherical models (Moors et al, 2007;Moors et al, 2009;Schreiber et al, 2010;tegenge et al, 2010).…”

Section: Potential Of Non-mammalian Species As Relevant Dnt Modelsmentioning

confidence: 99%

See 1 more Smart Citation

Advancing the science of developmental neurotoxicity (DNT): testing for better safety evaluation

Bal‐Price

2012

ALTEX

View full text Add to dashboard Cite

“…They also observed an inward Ca 2+ current but they did not show the raw data (Piper et al, 2000). Among the currents reported by Piper et al, the K + outward currents and the Na + inward currents have also been investigated in many other studies using hNSCs from different sources (Cho et al, 2002;Sun et al, 2005;Johnson et al, 2007). This is due to the fact that these currents are regarded as markers for functionally differentiated neurons in terms of generation and repolarization of action potential.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Ionic Currents in Human Neural Stem Cells

Lim

Kim

Suh‐Kim

et al. 2008

Korean J Physiol Pharmacol

View full text Add to dashboard Cite

The profile of membrane currents was investigated in differentiated neuronal cells derived from human neural stem cells (hNSCs) that were obtained from aborted fetal cortex. W hole-cell voltage clamp recording revealed at least 4 different currents: a tetrodotoxin (TTX)-sensitive Na + current, a hyperpolarization-activated inward current, and A-type and delayed rectifier-type K + outward currents. Both types of K + outward currents were blocked by either 5 mM tetraethylammonium (TEA) or 5 mM 4-aminopyridine (4-AP). The hyperpolarization-activated current resembled the classical K + inward current in that it exhibited a voltage-dependent block in the presence of external Ba 2+ (30μ M) or Cs + (3μ M). However, the reversal potentials did not match well with the predicted K + equilibrium potentials, suggesting that it was not a classical K + inward rectifier current. The other Na + inward current resembled the classical Na + current observed in pharmacological studies. The expression of these channels may contribute to generation and repolarization of action potential and might be regarded as functional markers for hNSCs-derived neurons.

show abstract

Voltage‐Sensitive and Ligand‐Gated Channels in Differentiating Neural Stem–Like Cells Derived from the Nonhematopoietic Fraction of Human Umbilical Cord Blood

Cited by 90 publications

References 81 publications

Viability and neural differentiation of mesenchymal stem cells derived from the umbilical cord following perinatal asphyxia

Viability and neural differentiation of mesenchymal stem cells derived from the umbilical cord following perinatal asphyxia

Advancing the science of developmental neurotoxicity (DNT): testing for better safety evaluation

Characterization of Ionic Currents in Human Neural Stem Cells

Contact Info

Product

Resources

About