Volatiles produced by interacting microorganisms potentially useful for the control of plant pathogens

Campos, Vicente Paulo; Pinho, Renata Silva Canuto de; Freire, Eduardo Souza

doi:10.1590/s1413-70542010000300001

Cited by 65 publications

(37 citation statements)

References 47 publications

(66 reference statements)

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“…The production of volatile emissions by E. nigrum, S. commune, P. chrysosporium, Nisgrospora sp., and Lasiodiplodia sp. toxic to nematodes have not yet been described; however, many other fungi species produce volatiles toxic to plant pathogenic nematode and to other plant pathogens (Campos et al, 2010;Huang et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Wood-associated fungi produce volatile organic compounds toxic to root-knot nematode

Pimenta

Ferreira

Pedroso

et al. 2017

Sci. agric. (Piracicaba, Braz.)

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Since volatiles do not leave residues in food and cause low toxicity to humans, they can act as a skeleton for new nematicidal molecules, once they have demonstrated activity against phytonematodes. The aim of this study was to evaluate the effect of volatile organic compounds (VOCs) released by wood-associated fungi on controling second-stage juveniles (J2) of Meloidogyne incognita. All 28 wood-associated fungi isolates produced VOCs which caused in excess of 76 % immobility in exposed J2 of M. incognita. The fungus isolate VOCs also caused significant mortality compared to control when J2 were exposed to them. After 6 h of exposure, the fungus species Epicoccum nigrum and Schizophyllum commune produced VOCs that immobilized exposed J2 compounds, in relation to the control. When J2 were inoculated into tomato under greenhouse conditions, the M. incognita infectivity and reproduction were reduced by exposure to the VOCs. Volatiles produced by most of the fungi isolates and analyzed by gas chromatograph coupled to a mass spectrometer (CG-MS), included alcohols, esters, terpenes, and ketones. Certain compounds appeared only in S. commune or in E. nigrum suggesting their involvement in the high level of damage caused to nematode reproduction on tomato. Volatiles emitted by wood fungi demonstrated another mode of action of these microorganisms in nature.

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Section: Discussionmentioning

confidence: 99%

Wood-associated fungi produce volatile organic compounds toxic to root-knot nematode

Pimenta

Ferreira

Pedroso

et al. 2017

Sci. agric. (Piracicaba, Braz.)

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“…Highly significant volatile-mediated effects of bacteria have been reported for various target organisms, including bacteria themselves (1)(2)(3)(4)(5), plants (5)(6)(7)(8)(9), and fungi (10)(11)(12). The research carried out to understand the nature of this volatile-mediated interaction of bacteria with plants and with other bacteria has focused so far on model organisms (e.g., Arabidopsis thaliana and Escherichia coli) and has enabled identification of some of the active compounds involved in the respective interactions, such as indole, 2,3-butanediol, dimethyl disulfide, hydrogen sulfide, and ammonia.…”

mentioning

confidence: 99%

Pseudomonas Strains Naturally Associated with Potato Plants Produce Volatiles with High Potential for Inhibition of Phytophthora infestans

Hunziker

Bönisch

Groenhagen

et al. 2015

Appl Environ Microbiol

178

128

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bBacteria emit volatile organic compounds with a wide range of effects on bacteria, fungi, plants, and animals. The antifungal potential of bacterial volatiles has been investigated with a broad span of phytopathogenic organisms, yet the reaction of oomycetes to these volatile signals is largely unknown. For instance, the response of the late blight-causing agent and most devastating oomycete pathogen worldwide, Phytophthora infestans, to bacterial volatiles has not been assessed so far. In this work, we analyzed this response and compared it to that of selected fungal and bacterial potato pathogens, using newly isolated, potato-associated bacterial strains as volatile emitters. P. infestans was highly susceptible to bacterial volatiles, while fungal and bacterial pathogens were less sensitive. Cyanogenic Pseudomonas strains were the most active, leading to complete growth inhibition, yet noncyanogenic ones also produced antioomycete volatiles. Headspace analysis of the emitted volatiles revealed 1-undecene as a compound produced by strains inducing volatile-mediated P. infestans growth inhibition. Supplying pure 1-undecene to P. infestans significantly reduced mycelial growth, sporangium formation, germination, and zoospore release in a dose-dependent manner. This work demonstrates the high sensitivity of P. infestans to bacterial volatiles and opens new perspectives for sustainable control of this devastating pathogen. During the last decade, it has become evident that bacteria communicate with other organisms through the emission of volatile compounds. Highly significant volatile-mediated effects of bacteria have been reported for various target organisms, including bacteria themselves (1-5), plants (5-9), and fungi (10-12). The research carried out to understand the nature of this volatile-mediated interaction of bacteria with plants and with other bacteria has focused so far on model organisms (e.g., Arabidopsis thaliana and Escherichia coli) and has enabled identification of some of the active compounds involved in the respective interactions, such as indole, 2,3-butanediol, dimethyl disulfide, hydrogen sulfide, and ammonia. The research on model organisms has also contributed to understanding of the mechanisms underlying the observed phenotypic changes of increased (13-15) or decreased (16, 17) plant biomass and increased antibiotic resistance in bacteria (2-4, 18).As far as fungi are concerned, most studies investigating their response to bacterial volatiles have focused on potential application and have thus largely neglected deeper investigation of the chemical nature of the active compounds and/or of the mode of action of these molecules. In addition to the inorganic volatiles hydrogen cyanide (19) and ammonia (20), few volatile organic compounds, such as sulfur compounds and long-chain ketones, have been unequivocally shown to inhibit the growth of phytopathogenic fungi when applied at biologically relevant concentrations (12). With the ultimate prospect of using the antifungal potential of bacterial...

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“…aerius IPB 1 berhasil menghambat pertumbuhan koloni B. theobromae pada mangga selama masa penyimpanan. Khamir Williopsis mrakii menghambat pertumbuhan B. theobromae sampai 100% (Campos et al 2010) melalui produksi senyawa volatil.…”

Section: Pembahasanunclassified

Mekanisme Pengendalian Penyakit Busuk Batang Jeruk oleh Khamir, Kitosan, Cendawan Mikoriza Arbuskular, dan Bakteri Simbiotiknya

Khairani

Sinaga

Mutaqin

2017

J Fitopatol Indones

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ABSTRAKProduktivitas tanaman jeruk seringkali dibatasi oleh penyakit tanaman. Di Indonesia, penyakit busuk batang yang disebabkan oleh Botryodiplodia theobromae menjadi penyakit penting yang menyebabkan tanaman jeruk mati meranggas. Penelitian dilakukan untuk mempelajari mekanisme pengendalian B. theobromae oleh khamir, cendawan mikoriza arbuskular (CMA), bakteri simbiotik CMA dan kitosan. Uji in vitro dilakukan untuk mengevaluasi mekanisme antibiosis, hiperparasitisme, produksi senyawa volatil, dan produksi enzim kitinase. Percobaan dilanjutkan dengan pengujian in planta terhadap khamir, CMA, bakteri simbiotik CMA terpilih, dan kitosan baik secara tunggal maupun kombinasi. Percobaan disusun dalam rancangan acak lengkap dengan 3 ulangan. Peubah yang diamati ialah periode laten, insidensi dan keparahan penyakit, laju infeksi, area under disease progress curve (AUDPC), tingkat asosiasi CMA, dan kandungan fenol total. Hasil uji in vitro menunjukkan bahwa khamir mempunyai aktivitas hiperparasitisme pada B. theobromae dengan afinitas 26 sel per hifa, memproduksi senyawa volatil dengan tingkat hambatan relatif (THR) 29.1%, dan memproduksi enzim kitinase. Isolat bakteri simbiotik CMA terbaik menunjukkan mekanisme antibiosis dengan THR 42.9% dan memproduksi senyawa volatil dengan THR 26.7%. Isolat ini memiliki homologi 98% dengan Bacillus subtilis asal Vietnam. Perlakuan khamir + CMA + kitosan memperlambat periode laten dan menekan insidensi penyakit. Perlakuan tunggal CMA dan kombinasi khamir + CMA menekan keparahan penyakit, laju infeksi, dan nilai AUDPC. Aplikasi khamir + CMA menunjukkan tingkat asosiasi CMA dan peningkatan kandungan fenol total paling tinggi. Berdasarkan hasil penelitian ini, perlakuan tunggal CMA dan khamir + CMA direkomendasikan dalam pengendalian penyakit busuk batang jeruk.Kata kunci: antibiosis, Botryodiplodia theobromae, hiperparasitisme, kitinase ABSTRACT Plant diseases become an important constraint on citrus production. Stem rot disease caused by Botryodiplodia theobromae is a major disease on citrus in Indonesia. This study was aimed to evaluate the mechanism of yeast, arbuscular mychorrhiza fungi (AMF), symbiotic bacteria of AMF and chitosan in controlling stem rot disease. In vitro study was performed to evaluate the mechanism of antibiosis, hyperparasitism, production of volatile compounds, and production of chitinase enzyme. The experiment was continued by in planta assays using yeast, AMF, symbiotic bacteria of AMF, and chitosan either singly or in combination. The experiments were performed using completely randomized

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Volatiles produced by interacting microorganisms potentially useful for the control of plant pathogens

Cited by 65 publications

References 47 publications

Wood-associated fungi produce volatile organic compounds toxic to root-knot nematode

Wood-associated fungi produce volatile organic compounds toxic to root-knot nematode

Pseudomonas Strains Naturally Associated with Potato Plants Produce Volatiles with High Potential for Inhibition of Phytophthora infestans

Mekanisme Pengendalian Penyakit Busuk Batang Jeruk oleh Khamir, Kitosan, Cendawan Mikoriza Arbuskular, dan Bakteri Simbiotiknya

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