Vitrification of mouse oocytes results in aneuploid zygotes and malformed fetuses

Kola, Ismail; Kirby, Catherine; Shaw, Jillian M; Davey, Anna; Trounson, Alan

doi:10.1002/tera.1420380510

Cited by 112 publications

(50 citation statements)

References 20 publications

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“…Contrary to the complete cryopreservation process (Kola et al, 1988;Carroll et al, 1989;Bouquet et al, 1992; present study), the exposure of oocytes to cooling to 0°C and/or to DMSO does not induce an increased total number of morphological abnormalities. This result, which is in accordance with the observations of Glenister et al (1987), suggests that the important cellular damage after oocyte cryopreservation is due to events taking place at the time of freezing from 0°C to -196°C andlor thawing.…”

Section: Morphological Abnormalitiescontrasting

confidence: 74%

Effects of cooling and equilibration in DMSO, and cryopreservation of mouse oocytes, on the rates of in vitro fertilization, development, and chromosomal abnormalities

Bouquet

Selva

Auroux

1995

Molecular Reproduction Devel

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In a previous study, we have shown that the cryopreservation of mouse oocytes caused increases in the rates of degeneration and of digynic polyploid embryos, while the fertility of frozen-thawed oocytes was decreased. In this study, we have attempted to determine the different stages in the complete freezing-thawing process which are deleterious for the oocytes and the subsequent zygotes. IVF assays showed that DMSO decreased the fertility of oocytes, whereas cooling to 0 degrees C had no effect. DMSO, used at 0 degrees C, was less deleterious for oocytes. Thus, the prefreezing manipulations seem to be important for the quality and fertility of oocytes. However, neither DMSO nor cooling increased the incidence of chromosomal abnormalities in embryos obtained from inseminated exposed oocytes. Therefore, the increased frequency of polyploidy observed in embryos after the cryopreservation of mouse oocytes must correspond to disruption occurring during the freezing-thawing process.

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Section: Morphological Abnormalitiescontrasting

confidence: 74%

Effects of cooling and equilibration in DMSO, and cryopreservation of mouse oocytes, on the rates of in vitro fertilization, development, and chromosomal abnormalities

Bouquet

Selva

Auroux

1995

Molecular Reproduction Devel

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“…In contrast, Kola et al (1988) after analyzing a much smaller sample of mouse embryos at first cleavage detected almost a three fold increase in aneuploidy in embryos derived from frozen-thawed oocytes.…”

Section: Introductionmentioning

confidence: 82%

Analysis of the chromosome complement of frozen‐thawed mouse oocytes after parthenogenetic activation

Bös-Mikich

Whittingham

1995

Molecular Reproduction Devel

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Frozen-thawed mouse oocytes were artificially activated with Sr2+ and analyzed cytogenetically at the first cleavage division to examine the behavior of the maternal chromosomes independently of the paternal complement. There was no significant difference in the rate of activation between frozen-thawed and freshly collected oocytes and the majority of oocytes (> 90%) had a normal haploid chromosome constitution. The incidence of second polar body retention in frozen-thawed oocytes was low and did not differ significantly from that observed in fresh oocytes and oocytes exposed to dimethylsulfoxide (DMSO) at 0 degree C or 37 degrees C for extended periods beyond those required for protection. The frequency of aneuploidy was similar for frozen-thawed and fresh oocytes but oocytes held at 0 degree C without DMSO or held at 37 degrees C with DMSO for 1 hr showed a 2.5 and 12-fold increase in the frequency of aneuploidy compared with oocytes subjected to a conventional oocyte/embryo freezing regime. It is concluded that the procedures used in successful oocyte cryopreservation do not increase the incidence of chromosomal abnormalities of maternal origin in the resulting embryos.

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“…Both DMSO and PROH have higher membrane permeability than glycerol [52][53][54]. However, it has been reported that DMSO causes spindle polymerization in oocytes with increased potential for polyploidy [35,[56][57][58][59]. In addition, it has been known that the toxicity of DMSO for oocytes and embryos is related to the duration and temperature at which exposure occurs, indicating that oocytes in which are exposed to DMSO at lower temperatures have a better outcome [39,[60][61].…”

Section: Discussionmentioning

confidence: 99%

High Survival Rate of Bovine Oocytes Matured In Vitro Following Vitrification

Chian

Kuwayama

Tan

et al. 2004

Journal of Reproduction and Development

165

100

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Abstract. Improving pregnancy rates associated with the use of cryopreserved human oocytes would be an important advance in human assisted reproductive technology (ART). Vitrification allows glasslike solidification of a solution without ice crystal formation in the living cells. We have attempted to improve the survival rates of oocytes by a vitrification technique using bovine models. In vitro matured oocytes with or without cumulus cells were vitrified with either 15.0% (v/v) ethylene glycol (EG) + 15% (v/v) dimethylsulfoxide (DMSO) + 0.5 M sucrose or 15% (v/v) EG + 15% (v/v) 1,2-propanediol (PROH) + 0.5 M sucrose, using 'Cryotop' or 'thin plastic sticker', respectively. The oocyte survival rates after vitrifying-warming, and the capacity for fertilization and embryonic development were examined in vitro. The rate of embryonic development to blastocyst was significantly higher (P<0.05) in the oocytes vitrified with 15% (v/v) EG + 15% (v/v) PROH + 0.5 M sucrose than in the oocytes vitrified with 15% (v/v) EG + 15% (v/v) DMSO + 0.5 M sucrose (7.4% ± 4.1 vs. 1.7% ± 3.0, respectively). Oocytes vitrified without cumulus cells had a higher survival rate after thawing and a superior embryonic developmental capacity compared with oocytes vitrified with cumulus cells. Prolonged pre-incubation time after thawing adversely affected the rates of embryonic cleavage and development. These results indicate that in vitro matured bovine oocytes can be vitrified successfully with the mixture of the cryoprotectants, EG + PROH, the absence of cumulus cells for vitrification does not affect oocyte survival rate after warming, and vitrified and warmed oocytes do not require pre-incubation before in vitro fertilization.

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Vitrification of mouse oocytes results in aneuploid zygotes and malformed fetuses

Cited by 112 publications

References 20 publications

Effects of cooling and equilibration in DMSO, and cryopreservation of mouse oocytes, on the rates of in vitro fertilization, development, and chromosomal abnormalities

Effects of cooling and equilibration in DMSO, and cryopreservation of mouse oocytes, on the rates of in vitro fertilization, development, and chromosomal abnormalities

Analysis of the chromosome complement of frozen‐thawed mouse oocytes after parthenogenetic activation

High Survival Rate of Bovine Oocytes Matured In Vitro Following Vitrification

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