Analysis of the chromosome complement of frozen‐thawed mouse oocytes after parthenogenetic activation

Bös-Mikich, Adriana; Whittingham, D. G.

doi:10.1002/mrd.1080420215

Cited by 19 publications

(9 citation statements)

References 31 publications

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“…However, the majority of studies with frozen-thawed mouse oocytes have not found any significant increase in the frequency of aneuploidy at first cleavage metaphase after fertilization in vitro [6,20]. Recently, we have confirmed this with frozenthawed mouse oocytes, where parthenogenetic activation of the oocytes with Sr 2+ was used to study the behavior of the maternal chromosomes in the absence of the paternal complement [21].…”

Section: Introductionmentioning

confidence: 62%

“…The combined effects of the components of VSI or exposure to the cryoprotectants at room temperature for 15 min may have disrupted the spindle more profoundly than in the present study. In oocytes exposed to 1.5 M DMSO at 37 0 C for 1 h, the incidence of aneuploidy after parthenogenetic activation is increased significantly [21]. However, the data of Kola et al [14] show a preponderance of hypohaploid spreads, which may have resulted from the loss of chromosomes during preparation.…”

Section: Discussionmentioning

confidence: 83%

“…Oocytes were activated in Medium M16 [23] in which the calcium component was replaced by an equimolar (1.71 mM) amount of Sr 2+ , MgCl 2 was omitted, and the sodium chloride concentration was decreased by 10% to give an osmolarity of 262-269 mOsm and a pH of 7.2-7.4 (M16 + Sr2+; [21]). The medium was supplemented with crystalline BSA (ICN, Flow Laboratories; 4 mg/ml).…”

Section: Parthenogenetic Activationmentioning

confidence: 99%

“…In this paper, we report on the incidence of chromosome abnormalities in mouse oocytes vitrified with 6 M DMSO [16] after activation with Sr 2+ [21] and the developmental potential of the vitrified oocytes after fertilization in vitro.…”

Section: Introductionmentioning

confidence: 99%

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Cytogenetical Analysis and Developmental Potential of Vitrified Mouse Oocytes1

Bös-Mikich¹,

Wood²,

Candy³

et al. 1995

Biology of Reproduction

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Mature mouse oocytes were cryopreserved by vitrification in 6 M dimethyl sulfoxide (VS). After warming they were either artificially activated with strontium (Sr2+), and the incidence of chromosome non-disjunction was assessed at first cleavage metaphase; or they were fertilized in vitro, and postimplantation survival was examined at Day 15 of gestation. Similar proportions of vitrified and freshly collected oocytes were activated with Sr2+ (75% and 82%, respectively). The majority of activated oocytes extruded the second polar body and formed a single pronucleus ( > 90%). When the exposure time to VS was increased from 90 to 110 sec without cooling, a significant proportion of activated oocytes arrested at the pronuclear stage (30%), and chromosome condensation did not occur. The frequency of aneuploidy in vitrified and control oocytes was similar, but when exposure to VS without cooling was extended, aneuploidy and second polar body retention were significantly higher than those of controls (p < 0.05). The rates of fertilization of vitrified (85%) and control oocytes (92%) did not differ. After transfer, similar proportions of vitrified and control embryos implanted (68-80%) and formed normal fetuses (38-49%). We conclude that vitrification in 6 M dimethyl sulfoxide is a simple and safe procedure for the preservation of mouse oocytes provided that the time of exposure to the cryoprotectant is carefully controlled.

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Section: Introductionmentioning

confidence: 62%

Section: Discussionmentioning

confidence: 83%

Section: Parthenogenetic Activationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Cytogenetical Analysis and Developmental Potential of Vitrified Mouse Oocytes1

Bös-Mikich¹,

Wood²,

Candy³

et al. 1995

Biology of Reproduction

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“…The introduction of intracytoplasmic sperm injection made it possible to overcome zona hardening after cryopreservation (6). Furthermore, subsequent studies suggest that the spindle is capable of undergoing some repair after cryopreservation and that dispersed chromosomes are rare (12)(13)(14)(15)(16)(17).…”

mentioning

confidence: 98%

Exposure of mouse oocytes to 1,2-propanediol during slow freezing alters the proteome

Katz‐Jaffe

Larman

Sheehan

et al. 2008

Fertility and Sterility

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Parthenogenetic activation of Chinese hamster oocytes by chemical stimuli and its cytogenetic evaluation

Tateno

Kamiguchi

1997

Mol. Reprod. Dev.

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This study was undertaken parthenogenetically to activate Chinese hamster oocytes in vitro by chemical stimuli. Oocytes were exposed to five different chemical agents, ethanol (EtOH), strontium chloride (SrCl2), cycloheximide (CHX), phorbol ester (PMA), and ionophore A23187 (IA23). No parthenogenetic activation was observed in the oocytes treated with 8% EtOH for 8–11 min, 1.7 mM and 5.0 mM SrCl2 for 1 hr, 100 μM and 400 μM CHX for 2 hr, and 81 nM and 162 nM PMA for 5 min. In contrast, 89.7% of oocytes parthenogenetically extruded the second polar body in treatment with 3 μM IA23 for 5 min, but only 22.6% of them formed a pronucleus and developed to 2‐cell embryos. The remaining ova stopped their cell cycle immediately after completion of the second meiotic division. They had unichromatid chromosomes (monads), which are called MIII chromosomes. Treatment with 5 μM IA23 for 5 min was so deleterious that >90% of oocytes were degenerated. However, oocyte activation was significantly improved when the treatment with 3 μM IA23 for 5 min was followed by treatment with 8% EtOH for 10 min, 100 μM CHX for 2 hr, 81 nM PMA for 5 min or 3 μM IA23 for 5 min: rates of pronuclear formation were 54.4%, 84.3%, 34.2%, and 54.6%, respectively. More than 80% of pronucleate ova successfully developed into 2‐cell stage. Additive treatment with 5 mM SrCl2 for 1 hr had no positive effect on pronuclear formation. Incidences of aneuploidy (4.6%) and structural chromosome aberrations (1.0%) in parthenogenons produced by combined stimuli of IA23 and CHX were not significantly different from those (3.8% and 1.6%, respectively) in female pronuclei of ova fertilized in vitro, showing that combined treatments with IA23 and CHX cause neither nondisjunction at the second meiotic division nor structural aberrations in MII chromosomes. The present technique for parthenogenetic activation of Chinese hamster oocytes may be useful as an assessment system to detect aneugenic and clastogenic effects of mutagens on mammalian oocytes. Mol. Reprod. Dev. 47:72–78, 1997. © 1997 Wiley‐Liss, Inc.

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Analysis of the chromosome complement of frozen‐thawed mouse oocytes after parthenogenetic activation

Cited by 19 publications

References 31 publications

Cytogenetical Analysis and Developmental Potential of Vitrified Mouse Oocytes1

Cytogenetical Analysis and Developmental Potential of Vitrified Mouse Oocytes1

Exposure of mouse oocytes to 1,2-propanediol during slow freezing alters the proteome

Parthenogenetic activation of Chinese hamster oocytes by chemical stimuli and its cytogenetic evaluation

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