Effects of cooling and equilibration in DMSO, and cryopreservation of mouse oocytes, on the rates of in vitro fertilization, development, and chromosomal abnormalities

Bouquet, M.; Selva, J.; Auroux, M

doi:10.1002/mrd.1080400114

Cited by 45 publications

(25 citation statements)

References 33 publications

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“…Using low temperatures without cryoprotectant or exposing oocytes to cryoprotectant a t high temperatures are deleterious. Moreover, in our parthenogenetic system we did not detect any increase in the retention of a second polar body, as suggested before (Glenister et al, 1987;Carroll et al, 1989;Bouquet et al, 1992Bouquet et al, , 1995. However, the relatively high frequency (2-3%) with which this abnormality occurs in human fertilization (Boue et al, 1975) stresses the need of a careful evaluation of in vitro fertilized human embryos at the pronucleus stage before transfer to avoid the risk of polyspermy.…”

Section: Aneuploid Ycontrasting

confidence: 38%

Analysis of the chromosome complement of frozen‐thawed mouse oocytes after parthenogenetic activation

Bös-Mikich

Whittingham

1995

Molecular Reproduction Devel

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Frozen-thawed mouse oocytes were artificially activated with Sr2+ and analyzed cytogenetically at the first cleavage division to examine the behavior of the maternal chromosomes independently of the paternal complement. There was no significant difference in the rate of activation between frozen-thawed and freshly collected oocytes and the majority of oocytes (> 90%) had a normal haploid chromosome constitution. The incidence of second polar body retention in frozen-thawed oocytes was low and did not differ significantly from that observed in fresh oocytes and oocytes exposed to dimethylsulfoxide (DMSO) at 0 degree C or 37 degrees C for extended periods beyond those required for protection. The frequency of aneuploidy was similar for frozen-thawed and fresh oocytes but oocytes held at 0 degree C without DMSO or held at 37 degrees C with DMSO for 1 hr showed a 2.5 and 12-fold increase in the frequency of aneuploidy compared with oocytes subjected to a conventional oocyte/embryo freezing regime. It is concluded that the procedures used in successful oocyte cryopreservation do not increase the incidence of chromosomal abnormalities of maternal origin in the resulting embryos.

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Section: Aneuploid Ycontrasting

confidence: 38%

Analysis of the chromosome complement of frozen‐thawed mouse oocytes after parthenogenetic activation

Bös-Mikich

Whittingham

1995

Molecular Reproduction Devel

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“…We first determined the effect of TZP integrity on the rate of cleavage and development to the blastocyst stage using parthenogenetically activated (SrCl 2 +/-cytochalasin B) oocytes [9,25,37]. Diploid parthenotes cultured to blastocyst stage were used to assess the maternal contribu- (Table 1).…”

Section: Tzp Integrity During Ivm Affects Embryo Developmental Competmentioning

confidence: 99%

Cumulus cell contact during oocyte maturation in mice regulates meiotic spindle positioning and enhances developmental competence

Barrett

Albertini

2009

J Assist Reprod Genet

105

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Purpose To investigate the role of cumulus cell contact during oocyte maturation on meiotic spindle assembly and the acquisition of developmental competence. Methods Cumulus oocyte complexes isolated from mouse ovaries subjected to in vitro or in vivo maturation were analyzed by confocal microscopy with respect to oocyte somatic cell contacts and for their ability to develop after parthenogenic activation during embryo culture. Results Cell contact is maintained during maturation in vivo, predisposing oocytes to cortical meiotic spindle assembly and developmental competence acquisition. In contrast, oocytes matured in vitro lose cell contact coincident with central meiotic spindle assembly that results in cleavage delays upon egg activation and failure to form blastocysts. Experimental disruption of cell contact by the actin-depolymerizing agent latrunculin B results in the formation of enlarged meiotic spindles with dispersed chromosomes unlike the compact ordering of chromosomes observed on spindles formed after in vivo maturation, suggesting a link between cell contact and the acquisition of developmental competence. Conclusions Somatic cell contact optimizes oocyte quality during meiotic maturation by regulating the spatial organization and function of the meiotic spindle through actindependent mechanisms that enhance development.

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“…It has been found that DMSO is one of the most current cryoprotectants used for cryopreservation of germ cells in mammals (Bouquet et al, 1995). DMSO as a cryoprotectant has been successfully used for the cryopreservation of human oocytes (Hunter et al, 1991), somatic cells (Saeed et al, 2000), and bovine oocytes (Wu et al, 1999).…”

Section: Discussionmentioning

confidence: 99%

Vitrification of in vitro matured oocytes of Mangalica and Large White pigs

Varga

Gajdócsi

Makkosné

et al. 2008

Acta Veterinaria Hungarica

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The breeding of Mangalica, a native pig breed in Hungary, had been started in 1833, but this pig breed almost became extinct in Hungary in the past decades. In 1991, the number of sows was only 200. Although in these days the existing Mangalica population consists of more than 6000 animals representing different colour variations, the preservation of this traditional pig breed is still very important. Vitrification is a potential tool for the preservation of gametes and embryos of these animals. The aim of this study was to investigate the effects of vitrification on the developmental competence of Mangalica (M) and Large White (LW) oocytes following fertilisation. The oocytes were vitrified by the Open Pulled Straw (OPS) method using different concentrations of ethylene glycol and dimethyl sulphoxide as cryoprotectants. After rehydration the oocytes underwent in vitro fertilisation; the resultant zygotes were then cultured in vitro for four days to assess embryonic development. In the first experiment, in vitro maturation of M and LW oocytes was compared. No significant difference was observed in the nuclear maturation rate of LW and M oocytes. In the second experiment, the sensitivity of oocytes to vitrification was examined by evaluating oocyte morphology after thawing. A higher percentage of LW oocytes showed normal morphology compared to M oocytes, indicating that Mangalica oocytes are more sensitive to cryoprotectants than Large White oocytes. After warming and in vitro fertilisation, more than 50% of the oocytes started embryonic development and by the end of the incubation period morula stage embryos had developed in both groups. The results show that the OPS vitrification technique is well suited to preserve Mangalica oocytes and from these oocytes morula embryos can be produced.

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Effects of cooling and equilibration in DMSO, and cryopreservation of mouse oocytes, on the rates of in vitro fertilization, development, and chromosomal abnormalities

Cited by 45 publications

References 33 publications

Analysis of the chromosome complement of frozen‐thawed mouse oocytes after parthenogenetic activation

Analysis of the chromosome complement of frozen‐thawed mouse oocytes after parthenogenetic activation

Cumulus cell contact during oocyte maturation in mice regulates meiotic spindle positioning and enhances developmental competence

Vitrification of in vitro matured oocytes of Mangalica and Large White pigs

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