Vitrification: Morphological, Physiological, and Ecological Aspects

Gaspar, Th.; Kevers, Claire; Debergh, Pierre; Maene, L.; Pâques, M.; Boxus, P.

doi:10.1007/978-94-017-0994-1_10

Cited by 71 publications

(35 citation statements)

References 24 publications

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“…Talvez ela induza uma súbita atividade celular (BORNMAN & VOGELMANN, 1984;KATAEVA et al, 1991;WILLIAMS & RAJI, 1991) e em um meio com alto potencial hídrico e/ou uma atmosfera de elevada umidade relativa no interior dos frascos, resultaria em novas células que logo se turgiriam. Certos autores como GASPAR et al (1984) citam ainda a possibilidade dos reguladores provocarem um estresse com o posterior desencadeamento da síntese de etileno. Este último seria o responsável pelas alterações observadas na morfogênese anormal ao nível de parede celular.…”

Section: Resultsunclassified

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Controle da vitrificação do cravo (Dianthus caryophyllus L.) in vitro

Cuzzuol

Gallo

Almeida

et al. 1995

Sci. agric. (Piracicaba, Braz.)

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Section: Resultsunclassified

“…Relação inversa foi constatada por GASPAR et al (1984) em meios enriquecidos de cálcio para certas espécies lenhosas. Tais informações corroboram os resultados do presente trabalho no qual a freqüência da vitrificação elevou-se progressivamente de acordo com o aumento dos níveis de NH 4 NO 3 (Tabela 3).…”

Section: Resultsunclassified

Controle da vitrificação do cravo (Dianthus caryophyllus L.) in vitro

Cuzzuol

Gallo

Almeida

et al. 1995

Sci. agric. (Piracicaba, Braz.)

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“…The prime causes of this disorder were suspected to be the inappropriate osmotic potential or water potential of the culture medium (Debergh et al, 1981), high humidity, superfluous nutritional factors, high levels of growth regulators, low light intensity (Ziv, 1986;Gasper et al, 1987) and ethylene accumulation in the air space of the culture vessel (Mele et al, 1982). Addition of sucrose did not rectify this disorder, but lowering the total water potential by increasing the agar concentration to 1)1% produced normal shoots (Debergh et al, 1981).…”

Section: Discussionmentioning

confidence: 99%

Tissue culture technology for the conservation and propagation of certain native plants

Sudhersan

AboEl-Nil

Hussain

2003

Journal of Arid Environments

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“…The syndrome is characterized by fragile, pale green leaves that are almost translucent, a condition described previously as vitrification. At the cellular level, there are many defects in vitrified plants, including the lack of palisade cells, large vacuoles in spongy mesophyll, few stomata, low/ no lignification, and few vascular bundles and hypertrophy in stem parenchyma (Gaspar et al, 1987). Hyperhydricity is a stress-induced state where differentiation is restricted, and these plants appear to be attaining a state where they can survive in the presence of stress from culture.…”

Section: Discussionmentioning

confidence: 99%

Identification of Novel Growth Regulators in Plant Populations Expressing Random Peptides

et al. 2017

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The use of chemical genomics approaches allows the identification of small molecules that integrate into biological systems, thereby changing discrete processes that influence growth, development, or metabolism. Libraries of chemicals are applied to living systems, and changes in phenotype are observed, potentially leading to the identification of new growth regulators. This work describes an approach that is the nexus of chemical genomics and synthetic biology. Here, each plant in an extensive population synthesizes a unique small peptide arising from a transgene composed of a randomized nucleic acid sequence core flanked by translational start, stop, and cysteine-encoding (for disulfide cyclization) sequences. Ten and 16 amino acid sequences, bearing a core of six and 12 random amino acids, have been synthesized in Arabidopsis (Arabidopsis thaliana) plants. Populations were screened for phenotypes from the seedling stage through senescence. Dozens of phenotypes were observed in over 2,000 plants analyzed. Ten conspicuous phenotypes were verified through separate transformation and analysis of multiple independent lines. The results indicate that these populations contain sequences that often influence discrete aspects of plant biology. Novel peptides that affect photosynthesis, flowering, and red light response are described. The challenge now is to identify the mechanistic integrations of these peptides into biochemical processes. These populations serve as a new tool to identify small molecules that modulate discrete plant functions that could be produced later in transgenic plants or potentially applied exogenously to impart their effects. These findings could usher in a new generation of agricultural growth regulators, herbicides, or defense compounds.

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Vitrification: Morphological, Physiological, and Ecological Aspects

Cited by 71 publications

References 24 publications

Controle da vitrificação do cravo (Dianthus caryophyllus L.) in vitro

Controle da vitrificação do cravo (Dianthus caryophyllus L.) in vitro

Tissue culture technology for the conservation and propagation of certain native plants

Identification of Novel Growth Regulators in Plant Populations Expressing Random Peptides

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