Tissue culture technology for the conservation and propagation of certain native plants

An efficient in vitro plantlet regeneration of Cryptocoryne wendtii and Cryptocoryne becketti through shoot tip culture

“…HgCl 2 is often used in cases of difficulties with plant sterilization. Other plant species such as wild strawberry (Bhatt and Dhar 2000), olives (Zacchini and De Agazio 2004), Zizyphus spina-christi (Sudhersan et al 2003), Orthosiphon stamineus (Lee and Chan 2004) and Zingiberaceae species (Chan and Thong 2004) have also used HgCl 2 for surface sterilization procedure. About 20% 1.0 ± 0.0 a 0.9 ± 0.2 ab 3.0 ± 0.9 ab 1.3 ± 2.0 abcd 0.5 ± 0.6 abcd 0.2 0.0 2.5 ± 0.8 abcde 0.7 ± 0.1 ab 4.0 ± 0.6 ab 2.7 ± 0.8 de 1.6 ± 0.3 fg 0.2 0.2 2.7 ± 0.5 abcde 0.9 ± 0.2 ab 3.7 ± 0.5 ab 1.7 ± 0.5 abcd 1.0 ± 0.3 cdefg 0.2 0.5 2.8 ± 1.2 abcde 0.7 ± 0.2 ab 4.3 ± 0.5 ab 2.8 ± 2.6 de 0.7 ± 0.1 abcde 0.2 0.7 1.7 ± 0.5 abc 0.7 ± 0.2 ab 3.5 ± 0.8 ab 2.0 ± a0.9 bcd 0.7 ± 0.1 abcde 0.2 1.0 2.7 ± 1.0 abcde 0.9 ± 0.2 ab 4.7 ± 0.8 b 2.2 ± 0.8 bcd 0.6 ± 0.1 abcde 0.5 0.0 3.5 ± 1.9 abcde 0.7 ± 0.1 ab 3.5 ± 0.5 ab 1.5 ± 1.0 abcd 1.2 ± 0.6 defg 0.5 0.2 4.5 ± 1.9 e 0.7 ± 0.2 ab 3.5 ± 0.5 ab 2.3 ± 1.2 cde 1.4 ± 0.1 efg 0.5 0.5 4.5 ± 0.8 e 0.8 ± 0.2 ab 3.7 ± 1.0 ab 2.2 ± 0.8 bcd 0.9 ± 0.4 cdefg 0.5 0.7 4.5 ± 1.5 e 0.8 ± 0.1 ab 4.3 ± 0.5 ab 0.2 ± 0.4 ab 0.2 ± 0.5 abc 0.5 1.0 1.8 ± 1.2 abcd 0.7 ± 0.1 ab 4.5 ± 0.5 ab 0.2 ± 0.4 ab 0.1 ± 0.2 ab 0.7 0.0 3.7 ± 1.4 bcde 0.9 ± 0.2 ab 3.0 ± 1.3 ab 0.8 ± 0.8 abcd 0.4 ± 0.3 abcd 0.7 0.2 3.5 ± 1.4 abcde 0.8 ± 0.2 ab 4.5 ± 0.5 ab 2.0 ± 0.8 abcd 1.1 ± 0.3 defg 0.7 0.5 4.0 ± 1.8 cde 0.5 ± 0.0 a 4.3 ± 1.4 ab 0.8 ± 1.0 abcd 0.5 ± 0.6 abcd 0.7 0.7 3.3 ± 0.8 abcde 0.7 ± 0.1 ab 3.5 ± 1.0 ab 0.3 ± 0.8 abc 0.2 ± 0.4 ab 0.7 1.0 3.7 ± 1.4 bcde 0.9 ± 0.1 b 3.5 ± 0. of the aseptic cultures of both C. wendtii and C. becketti showed secondary contamination due to systemic bacteria after 6 weeks of culture.…”

Pests in an ancient Egyptian harbor

“…1 cm large and containing a single seed. Humans and animals eat fruits and young plants (Sudhersan et al, 2001), and a sweet concentrate made from the red drupes that produces a pleasant drink when diluted with cool water is consumed in Egypt (Osborn, 1968). Both seeds and fruits are consumed by Bedouins and animals in Sinai and the Negev (Bailey and Danin, 1981).…”

Thinking inside the Box: Tissue Culture for Plant Propagation in a Key Ecological Species, <i>Andropogon gerardii</i>

“…For non-model species, tissue culture techniques can make commercialization possible (e.g., indouglas-fir [23]). Yet, use of micropropagated plants is rarely suggested for ecological research [24].…”