Vitrification in Micropropagation

Gaspar, Thomas

doi:10.1007/978-3-642-76415-8_7

Cited by 41 publications

(33 citation statements)

References 59 publications

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“…An adverse consequence of culture with high cytokinin is that explant tissue can over-accumulate water giving the explant a glassy, vitrified appearance (Gaspar 1991). In this study, the dry weight, expressed as a percentage of the fresh weight, was greater in explants cultured without BA compared with those cultured with BA (Table 1).…”

Section: Resultsmentioning

confidence: 55%

Cytokinin-induced abnormal shoot organogenesis is associated with elevated Knotted1-type homeobox gene expression in tobacco

Ramage

Williams

2004

Plant Cell Rep

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The molecular mechanisms that regulate the transcription of key developmental genes involved in shoot organogenesis have yet to be fully elucidated. However, it is clear that plant growth regulators, such as cytokinin, play a critical role in the differentiation of adventitious shoots. In Nicotiana tabacum zz100 leaf discs, high frequency shoot formation could be induced with 5 microM of the cytokinin N6-benzyladenine (BA). Increasing the exogenous BA concentration to greater than 20 microM resulted in stunted explants with abnormal shoot morphology and altered mineral composition. Explants with abnormal shoots did not appear to be hyperhydric. Abnormalities were, however, associated with an increase in the expression of a knotted1-type homeobox gene (TobH1) isolated from normal shoot-forming cultures. The results suggest that the development of cytokinin-induced abnormal shoot morphology possibly involves changes in TobH1 gene expression.

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Section: Resultsmentioning

confidence: 55%

Cytokinin-induced abnormal shoot organogenesis is associated with elevated Knotted1-type homeobox gene expression in tobacco

Ramage

Williams

2004

Plant Cell Rep

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“…The reduced fresh weight of the bioreactorregenerated plants was possibly associated (in a causal relationship) to the fact that regenerants did not demonstrate hyperhydricity (as indicated by glassy appearance) and were successfully acclimatized (Gaspar 1991). Plant micropropagation in a liquid culture system is frequently associated with the phenomenon of hyperhydricity or, which results in malformed plants that cannot survive transplanting ex vitro (Chen and Ziv 2001).…”

Section: Discussionmentioning

confidence: 99%

The effect of ATP on cucumber (Cucumis sativus L.) regeneration from nodal explants: association with α-tocopherol, H2O2 and size of culture vessel

Matakiadis

Kintzios

2005

Plant Growth Regul

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We investigated the effect of adenosine 5¢-triphosphate (ATP), a-tocopherol and H 2 0 2 on the micropropagation of cucumber (Cucumis sativus L.) from nodal explants. The effect of the size of the liquid culture vessel (250-ml flask vs. 2.5-l airlift bioreactor) was also evaluated. The addition of ATP alone caused a significant increase in the number of branches (internodes) and in internodal length, but also a reduction of leaf number, compared to control cultures. It also increased significantly the accumulation of NO 3 À and K + . The application of ATP + a-tocopherol was associated with a significant increase in bud, internodal, leaf and petiole number, internodal, petiole and root length, as well as plant fresh weight, but reduced PO 4 3À and Ca 2+ accumulation. The combined application of ATP + a-tocopherol + H 2 O 2 was associated with maximum petiole length and increased plant fresh weight but reduced Ca 2+ accumulation. Compared to all other treatments, application of ATP + a-tocopherol in bioreactor-incubated cultures produced significantly larger plants, with an increased bud number, internode lenght and soluble carbohydrate concentration, but also with a reduced fresh weight, root length and reduced NO 3 À and PO 4 3À and Ca 2+ concentrations. These effects were associated with changes in the redox status of the regenerants, as well as dehydrogenase and peroxidase activities. The perspective for an application of ATP and antioxidants as novel, cost-efficient growth regulators in the micropropagation of this commercially important vegetable species is discussed.Abbreviations: Asc -ascorbate; ATP -adenosine 5¢-triphosphate; H 2 DCF-DA -2¢, 7¢-dichlorofluorescein diacetate; HPLC -high pressure liquid chromatography; MS -Murashige and Skoog basal medium (1962); PPFD -photosynthetic photon flux density; TTC -triphenyl-tetrazolium chloride

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“…Hyperhydricity is induced by the combined action of several physical and/or chemical factors of the culture environment (Gaspar, 1991). Although most plants can adapt to these environmental conditions, some of them become abnormal with a translucent aspect due to chlorophyll deficiency and high water content (Debergh, 1983;Gaspar, 1991).…”

Section: Introductionmentioning

confidence: 99%

“…Although most plants can adapt to these environmental conditions, some of them become abnormal with a translucent aspect due to chlorophyll deficiency and high water content (Debergh, 1983;Gaspar, 1991). The phenomenon has been considered as a morpho-physiology disorder and many physiological and biochemical changes have been observed (Ziv, 1991;Franck et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Influence of 6-Benzyladenine and gelling agent on the reduction of hyperhydricity in Tectona grandis L.

Quiala¹,

Jiménez-Tello²,

Barbón³

et al. 2014

Rev. Colomb. Biotecnol.

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Vitrification in Micropropagation

Cited by 41 publications

References 59 publications

Cytokinin-induced abnormal shoot organogenesis is associated with elevated Knotted1-type homeobox gene expression in tobacco

Cytokinin-induced abnormal shoot organogenesis is associated with elevated Knotted1-type homeobox gene expression in tobacco

The effect of ATP on cucumber (Cucumis sativus L.) regeneration from nodal explants: association with α-tocopherol, H2O2 and size of culture vessel

Influence of 6-Benzyladenine and gelling agent on the reduction of hyperhydricity in Tectona grandis L.

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