The effect of ATP on cucumber (Cucumis sativus L.) regeneration from nodal explants: association with α-tocopherol, H2O2 and size of culture vessel

Abstract

We investigated the effect of adenosine 5¢-triphosphate (ATP), a-tocopherol and H 2 0 2 on the micropropagation of cucumber (Cucumis sativus L.) from nodal explants. The effect of the size of the liquid culture vessel (250-ml flask vs. 2.5-l airlift bioreactor) was also evaluated. The addition of ATP alone caused a significant increase in the number of branches (internodes) and in internodal length, but also a reduction of leaf number, compared to control cultures. It also increased significantly the accumulation of NO 3 À and K + . The application of ATP + a-tocopherol was associated with a significant increase in bud, internodal, leaf and petiole number, internodal, petiole and root length, as well as plant fresh weight, but reduced PO 4 3À and Ca 2+ accumulation. The combined application of ATP + a-tocopherol + H 2 O 2 was associated with maximum petiole length and increased plant fresh weight but reduced Ca 2+ accumulation. Compared to all other treatments, application of ATP + a-tocopherol in bioreactor-incubated cultures produced significantly larger plants, with an increased bud number, internode lenght and soluble carbohydrate concentration, but also with a reduced fresh weight, root length and reduced NO 3 À and PO 4 3À and Ca 2+ concentrations. These effects were associated with changes in the redox status of the regenerants, as well as dehydrogenase and peroxidase activities. The perspective for an application of ATP and antioxidants as novel, cost-efficient growth regulators in the micropropagation of this commercially important vegetable species is discussed.Abbreviations: Asc -ascorbate; ATP -adenosine 5¢-triphosphate; H 2 DCF-DA -2¢, 7¢-dichlorofluorescein diacetate; HPLC -high pressure liquid chromatography; MS -Murashige and Skoog basal medium (1962); PPFD -photosynthetic photon flux density; TTC -triphenyl-tetrazolium chloride

Improvement of Growth and Yield of Black Cumin Plant by Active Dry Yeast and Some Vitamins

“…Finally, the improving effects of antioxidants on growth characters might be attributed to their positive action on enhancing cell divisions and protecting plant cells from free radicals that responsible for plant sencences (Raskin, 1992).The positive effect of α-tocopherol on plant growth is in harmony with many findings on earlier results from literature on some plant species i.e. Tanczos et al (1982)on rice, Matakiadis and Kintzios (2005) on cucumber plants.…”

Evolutionary origins of the purinergic signalling system

“…ATP, alone or in combination with antioxidants, is a growth regulator for the micropropagation of cucumber from nodal explants and has commercial potential (Matakiadis & Kintzios 2005). ATP is released by plant cells and acts to suppress a default death pathway, and some forms of pathogen‐induced cell death are mediated by the depletion of extracellular ATP (Chivasa et al.…”

Effect of Glutamine, Biotin and ADP on Micropropagation and Growth of Chrysanthemum hybridum, Gerbera jamesonii and Cordyline fruticosa In vitro

“…Some authors noted that addition of either ATP or ADP caused a significant increase in cell division of in vitro synchronized potato (Ipomea batatas L.), cucumber (Cucumis sativus L.) and duckweed (Spirodella polyrhiza L.) cell suspension cultures (Kintzios et al 2002). Others mentioned that addition of ATP had positive effect on cucumber (Cucumis sativus L.) regeneration from nodal explants (Matakiadis and Kintzios 2005). The authors observed significant increasing number of internodes and length, but also noted a reduction of leaf number as effect of ATP addition.…”