Vitexin inhibits inflammation in murine ovalbumin-induced allergic asthma

Venturini, Cláudio Luís; Macho, Antonio; Arunachalam, Karuppusamy; Almeida, Danielle Ayr Tavares de; Rosa, Suellen Iara Guirra; Pavan, Eduarda; Balogun, Sikiru Olaitan; Damazo, Amílcar Sabino; Martins, Domingos Tabajara de Oliveira

doi:10.1016/j.biopha.2017.10.073

Cited by 37 publications

(18 citation statements)

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“…Researches have showed that this flavone (400 μg/kg) reduces the influx of pulmonary neutrophils by 80% in mice exposed to lipopolysaccharide aerosols [13]. Recently, it has been demonstrated that vitexin (0.2, 1 and 5 mg/kg) inhibits inflammation in allergic asthma induced by ovalbumin [14]. However, a study of the metabolism of vitexin in rats showed that it is poorly absorbed in the gastrointestinal tract [15] and has low bioavailability [16,17].…”

Section: Introductionmentioning

confidence: 99%

“…In light of the pharmacological potential of vitexin as an antiinflammatory (mainly in the respiratory system, as previously cited) [13,14], the low aqueous solubility and consequently the low availability, the present study aims to prepare and characterize the vitexin/β-CD inclusion complex with the aim of improving its solubility. The resulting complex will be characterized by techniques such as Nuclear Magnetic Resonance (NMR), SEM (Scanning Electron Microscopy), Fourier Transform Infrared (FT-IR) and dissolution study in vitro.…”

Section: Introductionmentioning

confidence: 99%

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Inclusion of vitexin in β-cyclodextrin: preparation, characterization and expectorant/antitussive activities

Costa

Menezes

Almeida

et al. 2020

Heliyon

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The study aimed to include the isolated vitexin of Jatropha mutabilis in the β-cyclodextrin cavity to improve the solubility of this flavone. Its characterization was performed by techniques such as 1 H NMR/ROESY (Nuclear Magnetic Resonance Spectroscopy), FT-IR (Infrared Spectroscopy with Fourier Transform), SEM (Morphological analysis of IC by Scanning Electron Microscopy) and dissolution study in vitro . In addition, the following activities were evaluated in the animal models: expectorant, phenol red dosage in bronchoalveolar lavage and antitussive, cough induced by citric acid. In the characterization of the complex, interaction between hydrogens of ring B of vitexin and (H3) of β-CD was observed, in addition to changes in morphology. In the dissolution test, an increase in the rate of dissolution of vitexin was observed in the first 30 min for the CI vitexin/β-CD when compared with vitexin. Regarding the pharmacological activity, it was observed that the inclusion complex (IC) vitexin/β-CD in the equivalent doses of 0.2, 1 and 5 mg/kg of flavone presented higher expectorant activity when compared to vitexin (p < 0.05), suggesting increased bioavailability. As for the antitussive activity, both vitexin and the complex had similar effects and were dose independent. In the toxicity test using Artemia salina , vitexin and IC vitexin/β-CD were considered non-toxic. At last, the study efficacy of vitexin/β-CD IC as an expectorant and of vitexin as antitussive. All of these data are being described for the first time.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Inclusion of vitexin in β-cyclodextrin: preparation, characterization and expectorant/antitussive activities

Costa

Menezes

Almeida

et al. 2020

Heliyon

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“…Type 2 helper T ( 2) cells seem to play a pivotal role in immune dysfunction, which contributes to the development of asthma [5][6][7]. In addition, it has been observed that the 2-associated cytokines (e.g., interleukin-4 (IL-4), IL-5, and IL-13) were released by the airway epithelial cells.…”

Section: Introductionmentioning

confidence: 99%

Effects of Pelargonium sidoides and Coptis Rhizoma 2 : 1 Mixed Formula (PS + CR) on Ovalbumin‐Induced Asthma in Mice

Min

Park

Choi

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Pelargonium sidoides (PS) is traditionally used to treat respiratory and gastrointestinal infections, dysmenorrhea, and hepatic disorders in South Africa. Coptis Rhizoma (CR) is used to treat gastroenteric disorders, cardiovascular diseases, and cancer in East Asia. In the present study, we intended to observe the possible beneficial antiasthma effects of PS and CR on the ovalbumin- (OVA-) induced asthma C57BL/6J mice. Asthma in mice was induced by OVA sensitization and subsequent boosting. PS + CR (300 and 1,000 mg/kg; PO) or dexamethasone (IP) was administered once a day for 16 days. The changes in the body weight and gains, lung weights and gross inspections, total and differential cell counts of leukocytes in bronchoalveolar lavage fluid (BALF), serum OVA-specific immunoglobulin E (OVA-sIgE) levels, interleukin-4 (IL-4) and IL-5 levels in BALF and lung tissue homogenate, and IL-4 and IL-5 mRNA levels in lung tissue homogenates were analyzed with lung histopathology: mean alveolar surface area (ASA), alveolar septal thickness, numbers of inflammatory cells, mast cells, and eosinophils infiltrated in the alveolar regions, respectively. Significant increases in lung weights, total and differential cell counts of leukocytes in BALF, serum OVA-sIgE levels, and IL-4 and IL-5 levels in BALF and lung tissue homogenate were observed in OVA control as compared to those of intact control. In addition, OVA control showed a significant decrease in mean ASA and increases in alveolar septal thickness, numbers of inflammatory cells, mast cells, and eosinophils infiltrated in alveolar regions. However, these allergic and inflammatory asthmatic changes were significantly inhibited by PS + CR in a dose-dependent manner. In this study, PS + CR showed dose-dependent beneficial effects on OVA-induced asthma in mice through anti-inflammatory and antiallergic activities. Therefore, it is expected that PS + CR have enough potential as a new therapeutic agent or as an ingredient of a medicinal agent for various allergic and inflammatory respiratory diseases including asthma.

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“…Vitexin is an active component from medicinal plants such as the leaf of hawthorn which is a widely used conventional Chinese medicine[ 12 ]. Modern pharmacological studies show that Vitexin exerts a variety of pharmacological activities, including antioxidant and anti-inflammatory functions[ 13 – 18 ]. Vitexin reduced hypoxia-ischemia neonatal brain damage[ 19 ], acute myocardial ischemia/reperfusion injury[ 18 ], lipopolysaccharide (LPS)-induced islet cell injury[ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Vitexin attenuates lipopolysaccharide-induced acute lung injury by controlling the Nrf2 pathway

Liu

et al. 2018

PLoS ONE

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BackgroundA major feature of acute lung injury (ALI) is excessive inflammation in the lung. Vitexin is an active component from medicinal plants which has antioxidant and anti-inflammatory activities. Oxidative stress and inflammation play important roles in the pathophysiological processes in ALI. In the current study, we investigate the effect and potential mechanisms of Vitexin on lipopolysaccharide (LPS)-induced ALI.MethodsALI was induced by LPS intratracheal instillation in C57BL/6 wild-type mice and Nrf2 gene knocked down (Nrf2-/-) mice. One hour before LPS challenge, Vitexin or vehicle intraperitoneal injection was performed. Bronchoalveolar lavage fluid and lung tissues were examined for lung inflammation and injury at 24 h after LPS challenge.ResultsOur animal study’s results showed that LPS-induced recruitment of neutrophils and elevation of proinflammatory cytokine levels were attenuated by Vitexin treatment. Vitexin decreased lung edema and alveolar protein content. Moreover, Vitexin activated nuclear factor erythroid-2-related factor 2 (Nrf2), and increased the activity of its target gene heme oxygenase (HO)-1. The LPS-induced reactive oxygen species were inhibited by Vitexin. In addition, the activation of the nucleotide-binding domain and leucine-rich repeat PYD-containing protein 3 (NLRP3) inflammasome was suppressed by Vitexin. However, these effects of Vitexin were abolished in the Nrf2-/- mice. Our cell studies showed that Vitexin enhanced the expression of Nrf2 and HO-1 activity. Moreover, reactive oxygen species (ROS) and IL-1β productions were reduced in Vitexin-treated cells. However, knockdown of Nrf2 by siRNA in RAW cells reversed the benefit of Vitexin.ConclusionsVitexin suppresses LPS-induced ALI by controlling Nrf2 pathway.

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Vitexin inhibits inflammation in murine ovalbumin-induced allergic asthma

Cited by 37 publications

References 50 publications

Inclusion of vitexin in β-cyclodextrin: preparation, characterization and expectorant/antitussive activities

Inclusion of vitexin in β-cyclodextrin: preparation, characterization and expectorant/antitussive activities

Effects of Pelargonium sidoides and Coptis Rhizoma 2 : 1 Mixed Formula (PS + CR) on Ovalbumin‐Induced Asthma in Mice

Vitexin attenuates lipopolysaccharide-induced acute lung injury by controlling the Nrf2 pathway

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