Vitexin enhances osteoblast differentiation through phosphorylation of Smad and expression of Runx2 at in vitro and ex vivo

Kim, Kyeong-Min; Son, Hyo-Eun; Min, Hyeon-Young; Jang, Won‐Gu

doi:10.1007/s11033-020-05929-y

“… 49 The other flavonoids in the Binahong plant that function in socket wound healing are quercetin and vitexin. 50 – 52 Quercetin has the ability to reduce osteoclast formation through inhibiting IL-17 (interleukin-17), which is induced by RANKL (receptor activator of nuclear factor kappa-B ligand), and quercetin can also increase osteogenesis, angiogenesis, and function as an antioxidant. 50 , 51 Vitexin can affect bone formation by increasing osteoblast differentiation through p-Smad (phosphorylation-small mother againts) and Runx2 (runt-related transcription factor 2).…”

Section: Discussionmentioning

confidence: 99%

“… 50 , 51 Vitexin can affect bone formation by increasing osteoblast differentiation through p-Smad (phosphorylation-small mother againts) and Runx2 (runt-related transcription factor 2). 52 …”

Section: Discussionmentioning

confidence: 99%

Effects of 3% binahong (Anredera cordifolia) leaf extract gel on alveolar bone healing in post-extraction tooth socket wound in Wistar rats (Rattus norvegicus)

Hanafiah

¹

,

Hanafiah

²

,

Dohude

³

et al. 2022

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Background: Binahong (Anredera cordifolia (Ten.) STEENIS) is a widely available herbal plant in Indonesia and has been intensely researched for its healing abilities due to its biological activities, but few have studied its capability in accelerating hard tissue healing in post-extraction tooth sockets. The purpose of this study was to analyse the effects of 3% binahong leaf extract gel on alveolar bone healing in post-extraction sockets in Wistar rats. Methods: In this study, 48 male Wistar rats were randomly allocated to twelve groups. After the extraction of the left mandibular incisor, sockets in Group I to IV were given 3% binahong leaf extract gel, group V to VIII were given a control gel, and group IX to XII were given Gengigel® for 14 days. The residual socket volume (RSV) and fibroblast proliferation were observed on the 3rd, 7th, and 14th day post-extraction, while the osteoblast and osteocyte proliferation were observed on the 7th, 14th, and 28th day post-extraction. The RSV data were analysed using repeated measure ANOVA and one-way ANOVA, while the histopathological data were analysed using one-way ANOVA. Results: The results showed that the binahong group had the lowest RSV and the highest fibroblast proliferation compared to the other groups on the 7th day (p<0.05) and the highest osteoblast and osteocyte proliferation compared to the other groups on the 14th day (p<0.05). Conclusion: The experiment showed that 3% binahong leaf extract gel could accelerate wound closure, which was characterized by a greater decrease in the RSV value in comparison to the other treatment groups and could enhance alveolar bone healing by increasing the proliferation of fibroblasts, osteoblasts, and osteocytes.

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“…39 The other flavonoids in the binahong plant that function in socket wound healing are quercetin and vitexin. [40][41][42] Quercetin has the ability to reduce osteoclast formation through inhibiting IL-17 (interleukin-17), which is induced by RANKL (receptor activator of nuclear factor kappa-B ligand), and quercetin can also increase osteogenesis, angiogenesis, and function as an antioxidant. 40,41 Vitexin can affect bone formation by increasing osteoblast differentiation through p-Smad (phosphorylation-small mother againts) and Runx2 (runt-related transcription factor 2).…”

Section: Discussionmentioning

confidence: 99%

“…40,41 Vitexin can affect bone formation by increasing osteoblast differentiation through p-Smad (phosphorylation-small mother againts) and Runx2 (runt-related transcription factor 2). 42 The tannin in the binahong plant functions in fibroblast migration because it can increase VEGF (vascular endothelial growth factor) in the early phase of wound healing. 43 VEGF is a protein that plays a role in fibroblast migration.…”

Section: Discussionmentioning

confidence: 99%

Effects of 3% binahong (Anredera cordifolia) leaf extract gel on alveolar bone healing in post-extraction tooth socket wound in Wistar rats (Rattus norvegicus)

Hanafiah

¹

,

Hanafiah

²

,

Dohude

³

et al. 2021

View full text Add to dashboard Cite

Background: Binahong (Anredera cordifolia (Ten.) STEENIS) is a widely available herbal plant in Indonesia and has been intensely researched for its healing abilities due to its biological activities, but few have studied its capability in accelerating hard tissue healing in post-extraction tooth sockets. The purpose of this study was to analyse the effects of 3% binahong leaf extract gel on alveolar bone healing in post-extraction sockets in Wistar rats. Methods: In this study, 48 male Wistar rats were randomly allocated to twelve groups. After the extraction of the left mandibular incisor, sockets in Group I to IV were given 3% binahong leaf extract gel, group V to VIII were given a control gel, and group IX to XII were given Gengigel® for 14 days. The residual socket volume (RSV) and fibroblast proliferation were observed on the 3rd, 7th, and 14th day post-extraction, while the osteoblast and osteocyte proliferation were observed on the 7th, 14th, and 28th day post-extraction. The RSV data were analysed using repeated measure ANOVA and one-way ANOVA, while the histopathological data were analysed using one-way ANOVA. Results: The results showed that the binahong group had the lowest RSV and the highest fibroblast proliferation compared to the other groups on the 7th day (p<0.05) and the highest osteoblast and osteocyte proliferation compared to the other groups on the 14th day (p<0.05). Conclusion: The experiment showed that 3% binahong leaf extract gel could accelerate wound closure, which was characterized by a greater decrease in the RSV value in comparison to the other treatment groups and could enhance alveolar bone healing by increasing the proliferation of fibroblasts, osteoblasts, and osteocytes.

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“…Osteoblast differentiation is mediated by the phosphorylation of Smad1/5/9. 10,30 We investigated whether zingerone could mediate the phosphorylation of Smad1/5/9. Western blot analysis was used to determine the effect of zingerone on Smad1/5/9 phosphorylation in MC3T3-E1 cells.…”

Section: Zingerone Increases Osteoblast Differentiation By Stimulatin...mentioning

confidence: 99%

Zingerone stimulates osteoblast differentiation by increasing Smad1/5/9‐mediated HO‐1 expression in MC3T3‐E1 cells and primary mouse calvarial cells

Kim

¹

,

Lim

²

,

Jang

³

2022

Clin Exp Pharma Physio

Self Cite

3

0

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Zingerone is a non-volatile compound found mainly in dried ginger. Zingerone increases the expression of osteogenic markers and has antioxidant effects. A previous study showed that zingerone accelerated osteoblast differentiation by suppressing the expression of Smad7, a member of the inhibitory Smad (I-Smad) family. However, it is not known if zingerone can induce osteoblast differentiation by regulating Smad1/5/9, a member of the receptor-regulated Smad (R-Smad) family. In addition, osteoblast differentiation induced by Smad1/5/9 mediated increases in the expression of heme oxygenase 1 (HO-1) has not been reported. This study investigated the effects of zingerone on osteoblast differentiation and confirmed the relationship between Smad1/5/9 and HO-1. Zingerone increased the expression of osteogenic genes including runt-related transcription factor 2 (Runx2), distal-less homeobox (Dlx5) and osteocalcin (OC) and also promoted Smad1/5/9 phosphorylation. Interestingly, HO-1 expression was also elevated by zingerone, and an inhibitor of HO-1 (Sn[IV] protoporphyrin IX dichloride [SnPP]) suppressed the zingeroneinduced increase in HO-1 expression and expression of osteogenic marker genes such as Dlx5, Runx2 and OC. Protein phosphatase 2A Cα (PP2A Cα, an inhibitor of Smad1/5/9) suppressed the zingerone-induced increase in HO-1 expression and expression of osteogenic marker genes. The zingerone-induced increase in HO-1 luciferase activity was suppressed by PP2A Cα. Taken together; our data demonstrate that zingerone promotes osteoblast differentiation by increasing Smad1/5/9 mediated HO-1 expression.

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Vitexin enhances osteoblast differentiation through phosphorylation of Smad and expression of Runx2 at in vitro and ex vivo

Cited by 12 publications

References 20 publications

Effects of 3% binahong (Anredera cordifolia) leaf extract gel on alveolar bone healing in post-extraction tooth socket wound in Wistar rats (Rattus norvegicus)

Effects of 3% binahong (Anredera cordifolia) leaf extract gel on alveolar bone healing in post-extraction tooth socket wound in Wistar rats (Rattus norvegicus)

Effects of 3% binahong (Anredera cordifolia) leaf extract gel on alveolar bone healing in post-extraction tooth socket wound in Wistar rats (Rattus norvegicus)

Zingerone stimulates osteoblast differentiation by increasing Smad1/5/9‐mediated HO‐1 expression in MC3T3‐E1 cells and primary mouse calvarial cells

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