Vitellibacter aquimaris sp. nov., a marine bacterium isolated from seawater

Abstract

A Gram-staining-negative, aerobic, yellow-orange-pigmented, rod-shaped bacterium designated D-24 T was isolated from seawater from sandy shoreline in Johor, Malaysia. The genus Vitellibacter is a member of the family Flavobacteriaceae in the phylum Bacteroidetes. The genus was first proposed by Nedashkovskaya et al. . Members of this genus are typically Gram-staining-negative, rodshaped, aerobic bacteria that have been isolated from marine environments, except for strain V. soesokkakensis RSSK-12 T which was isolated from the junction between the ocean and a freshwater spring. Menaquinone-6 (MK-6) was found to be the only respiratory quinone in all species of the genus Vitellibacter (Kim et al., 2010). Generally, the members of this genus possess iso-C 15 : 0 and iso-C 17 : 0 3-OH as major fatty acids and have a DNA G+C content in the range of 38.9-48.7 mol% . The current study aimed to determine the taxonomic position of strain D-24 T , based on phenotypic, genotypic and chemotaxonomic analyses.

Aequorivita sinensis sp. nov., isolated from sediment of the East China Sea, and reclassification of Vitellibacter todarodis as Aequorivita todarodis comb. nov. and Vitellibacter aquimaris as Aequorivita aquimaris comb. nov.

“…The predominant cellular fatty acids (>10 % of total fatty acids) of strain S1-10 T were iso-C 15 : 0 (43.2 %) and anteiso-C 15 : 0 (21.3 %). Thus, the fatty acid profiles of strain S1-10 T resembled those of other Aequorivita species [9,31,32]. However, strain S1-10 T contained lower amounts of iso-C 15 : 1 G and iso-C 15 : 0 and higher amounts of anteiso-C 15 : 0 compared to A. viscosa DSM 26349 T .…”

“…Hahnke et al [3] proposed to place Vitellibacter into Aequorivita and to emend the description of Aequorivita accordingly in 2016. At that time, the paper describing V. aquimaris was just published [31] and V. todarodis has not been described [32]. The reconstructed phylogenetic trees based on the NJ and ML algorithms revealed that V. aquimaris and V. todarodis were grouped with the members of the genus Aequorivita.…”

Aequorivita lutea sp. nov., a novel bacterium isolated from the estuarine sediment of the Pearl River in China, and transfer of Vitellibacter todarodis and Vitellibacter aquimaris to the genus Aequorivita as Aequorivita todarodis comb. nov. and Aequorivita aquimaris comb. nov.

“…Basonym: Vitellibacter aquimaris Thevarajoo et al 2016 The description is the same as for Vitellibacter aquimaris [10]. The type strain is D-24 T =KCTC 42708 T =DSM 101732 T .…”

“…The proposed names of type species Vitellibacter vladivostokensis KMN 3516 T [5], Vitellibacter aestuarii JC2436 T [6], Vitellibacter soesokkakensis RSSK-12 T [7], Vitellibacter nionensis VBW088 T [8] and Vitellibacter echinoideorum CC-CZW007 T [9], were amended as Aequorivita vladivostokensis KMN 3516 T , Aequorivita aestuarii JC2436 T , Aequorivita soesokkakensis RSSK-12 T , Aequorivita nionensis VBW088 T and Aequorivita echinoideorum CC-CZW007 T , respectively [4]. Two recently identified species in the genus of Vitellibacter, Vitellibacter aquimaris [10] and Vitellibacter todarodis [11], were not classified into the genus Aequorivita, even though they phylogenetically grouped with those type strains being moved to the genus Aequorivita from the genus Vitellibacter. In this study, the novel strain q18 T , isolated from the estuarine sediment of the Pearl River in China (113.7145° E, 22.1221° N) at the depth of 8 m, was characterized using a polyphonic approach.…”

“…Temperature, pH and salinity suitable for the growth of strain q18 T and the reference strain A. echinoideorum CC-CZW007 T were measured as described previously [20,21]. Growth at different temperatures (4,10,15,20,25,30,35,40,45 and 50 °C) of strain q18 T was determined in 2216E marine broth medium. The test of salinity requirements was based on different NaCl concentrations adjusted from 0 to 15 % (w/v) with intervals of 1 % at 25 °C.…”