Aequorivita sinensis sp. nov., isolated from sediment of the East China Sea, and reclassification of Vitellibacter todarodis as Aequorivita todarodis comb. nov. and Vitellibacter aquimaris as Aequorivita aquimaris comb. nov.

Wang, Qian; Cai, Sheng-Dong; Liu, Jie; Zhang, De-Chao

doi:10.1099/ijsem.0.004174

“…The major cellular fatty acids are iso-C 15:0 , iso-C 17:0 3-OH, iso-C 15:1 G, and summed feature 3 (C 16:1 ω7c and/or C 16:1 ω6c). The DNA G + C Table 3 Differential properties of strain A3-108 T and its adjacent genera Strains/species: 1, strain A3-108 T (this study); 2, Marinirhabdus (Wu et al 2016;Yang et al 2018); 3, Galbibacter (Li et al 2013;Shams et al 2007); 4, Marixanthomonas (Romanenko et al 2007); 5, Aureisphaera (Yoon et al 2015(Yoon et al , 2016; 6, Aequorivita (Bowman 2002;Kim et al 2010Kim et al , 2018Lin et al 2015;Liu et al 2013;Nedashkovskaya et al 2003;Park et al 2009Park et al , 2014Rajasabapathy et al 2015;Thevarajoo et al 2016;Wang et al 2020;Zhang et al 2020). + positive, − negative, v positive or negative, NA no data available ‡ NM non-motile, GM gliding motility *Y yellow, LY lemon-yellow, PY pale-yellow, BY bright-yellow, YO yellow-orange, O orange, VOY vivid orange yellow † R rod, C coccus, O ovoid, F filaments § SF3 Summed feature 3, SF9 Summed feature 9, I15:0 iso-C 15:0 , I15:1G iso-C 15:1 G, I17:0O iso-C 17:0 3-OH, I16:0 iso-C 16: 0 , I15:1 iso-C 15: 1 , I16:0O iso-C 16: 0 3-OH, A17:0O anteiso-C 17: 0 3-OH, 16:0 C 16:0 , 18:0 C 18:0 , A15:0 anteiso-C 15:0 , I15:0 iso-C 15:0 , I17:1ω9c iso-C 17: 1 ω9c, I17:1ω5c iso-C17: 1ω5c, I15:1ω10c iso-C 15: 1 ω10c, A15:1ω10c anteiso-C 15: 1 ω10c, I16:1ω6c iso-C 16: 1 ω6c NaCl range (%) 0.5-10 0.5-7.5 0-9 3.0-5.0 0.5-5.5 0-12 Optimum NaCl (%) 1.0 2.0-5.0 1.0-5.0 1-12 3 1-6 pH range 6.0-8.5 4.0-8.5 NA 6.5-8.5 7.0-9.0 5.0-10.…”

Section: Discussionmentioning

confidence: 90%

“… Strains/species: 1, strain A3-108 T (this study); 2, Marinirhabdus (Wu et al 2016 ; Yang et al 2018 ); 3, Galbibacter (Li et al 2013 ; Shams et al 2007 ); 4, Marixanthomonas (Romanenko et al 2007 ); 5, Aureisphaera (Yoon et al 2015 , 2016 ); 6, Aequorivita (Bowman 2002 ; Kim et al 2010 , 2018 ; Lin et al 2015 ; Liu et al 2013 ; Nedashkovskaya et al 2003 ; Park et al 2009 , 2014 ; Rajasabapathy et al 2015 ; Thevarajoo et al 2016 ; Wang et al 2020 ; Zhang et al 2020 ). + positive, − negative, v positive or negative, NA no data available ‡ NM non-motile, GM gliding motility * Y yellow, LY lemon-yellow, PY pale-yellow, BY bright-yellow, YO yellow-orange, O orange, VOY vivid orange yellow † R rod, C coccus, O ovoid, F filaments § SF3 Summed feature 3, SF9 Summed feature 9, I15:0 iso-C 15:0 , I15:1G iso-C 15:1 G, I17:0O iso-C 17:0 3-OH, I16:0 iso-C 16: 0 , I15:1 iso-C 15: 1 , I16:0O iso-C 16: 0 3-OH, A17:0O anteiso-C 17: 0 3-OH, 16:0 C 16:0 , 18:0 C 18:0 , A15:0 anteiso-C 15:0 , I15:0 iso-C 15:0 , I17:1ω9c iso-C 17: 1 ω 9 c , I17:1ω5c iso-C17: 1 ω 5 c , I15:1ω10c iso-C 15: 1 ω 10 c , A15:1ω10c anteiso-C 15: 1 ω 10 c , I16:1ω6c iso-C 16: 1 ω 6 c ¶ PE phosphatidylethanolamine, APL aminophospholipid, AL aminolipid, L lipid, LPE lysophosphatidylethanolamine, SL sphingolipid, PL phospholipid, PGL phosphoglycolipid …”

Section: Resultsmentioning

confidence: 99%

“…Strains/species: 1, strain A3-108 T (this study); 2, Marinirhabdus (Wu et al 2016 ; Yang et al 2018 ); 3, Galbibacter (Li et al 2013 ; Shams et al 2007 ); 4, Marixanthomonas (Romanenko et al 2007 ); 5, Aureisphaera (Yoon et al 2015 , 2016 ); 6, Aequorivita (Bowman 2002 ; Kim et al 2010 , 2018 ; Lin et al 2015 ; Liu et al 2013 ; Nedashkovskaya et al 2003 ; Park et al 2009 , 2014 ; Rajasabapathy et al 2015 ; Thevarajoo et al 2016 ; Wang et al 2020 ; Zhang et al 2020 ). + positive, − negative, v positive or negative, NA no data available…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Luteirhabdus pelagi gen. nov., sp. nov., a novel member of the family Flavobacteriaceae, isolated from the West Pacific Ocean

Ren

¹

,

Meng

²

,

Guo

³

et al. 2021

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A Gram-stain-negative, aerobic, and yellow-pigmented bacterium, designated A3-108T, was isolated from seawater of the West Pacific Ocean. Cells were non-motile and rod-shaped, with carotenoid-type pigments. Strain A3-108T grew at pH 6.0–8.5 (optimum 6.5) and 15–40 °C (optimum 28 °C), in the presence of 0.5–10% (w/v) NaCl (optimum 1.0%). It possessed the ability to produce H2S. Based on the 16S rRNA gene analysis, strain A3-108T exhibited highest similarity with Aureisphaera salina A6D-50T (90.6%). Phylogenetic analysis shown that strain A3-108T affiliated with members of the family Flavobacteriaceae and represented an independent lineage. The principal fatty acids were iso-C15:0, iso-C17:0 3-OH, iso-C15:1 G, and summed feature 3 (C16:1ω7c and/or C16:1ω6c). The sole isoprenoid quinone was MK-6. The major polar lipids were phosphatidylethanolamine, one unidentified aminophospholipid, one unidentified aminolipid and one unidentified lipid. The ANIb, in silico DDH and AAI values among the genomes of strain A3-108T and three reference strains were 67.3–71.1%, 18.7–22.1%, and 58.8–71.4%, respectively. The G + C content was 41.0%. Distinctness of the phylogenetic position as well as differentiating chemotaxonomic and other phenotypic traits revealed that strain A3-108T represented a novel genus and species of the family Flavobacteriaceae, for which the name Luteirhabdus pelagi gen. nov., sp. nov. is proposed (type strain, A3-108T = CGMCC 1.18821T = KCTC 82563T).

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“…Most recently, Aequorivita lutea q18 T was isolated from estuarine sediment of the Pearl River in PR China [4], while Aequorivita sinensis S1-10 T was isolated from marine sediment of the East China Sea [5]. In addition, seven members of the genus of Vitellibacter were moved to the genus Aequorivita in 2016 [6] and 2020 [5], including Vitellibacter vladivostokensis KMM 3516 T [7], Vitellibacter aestuarii JC2436 T [8], Vitellibacter soesokkakensis RSSK-12 T [9], Vitellibacter nionensis VBW088 T [10], Vitellibacter echinoideorum CC-CZW007 T [11], Vitellibacter todarodis MYP2-2 T [12] and Vitellibacter aquimaris D-24 T [13]. Most of the type strains of the genus Aequorivita are aerobic, Gram-stain-negative, non-spore-forming, oxidase-positive or -negative, and generally grow at between 4 and 40 °C [1, 2, 6].…”

Section: Full-textmentioning

confidence: 99%

“…Genomic DNA was extracted from strain KX20305 T using a bacterial DNA kit (Tiangen Biotech). The 16S rRNA gene was amplified using the universal primers 27F and 1492R [5], cloned into the pMD18-T vector (TaKaRa) and sequenced with primers M13-47 and RV-M (Sangon Biotech) Similarity of the resulting 16S rRNA gene sequence of strain KX20305 T (1493 nt) to other microbial sequences was determined using the EzBioCloud server (www.ezbiocloud.net/) [14]. The 16S rRNA gene sequence of strain KX20305 T and those of related taxa were aligned using Clustal X 2.0 [15].…”

Section: S Rrna Gene Phylogenymentioning

confidence: 99%

Aequorivita iocasae sp. nov., a halophilic bacterium isolated from sediment collected at a cold seep field in the South China Sea

Zhang

¹

,

Wang

²

,

Cao

³

et al. 2022

International Journal of Systematic and Evolutionary Microbiology

9

0

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A moderately halophilic bacterium, designated strain KX20305T, was isolated from sediment collected from a cold seep field in the South China Sea. Cells of strain KX20305T were Gram-stain-negative, rod-shaped, non-motile, facultatively anaerobic, oxidase- and catalase-positive, and grew optimally at 25–30 °C, pH 6.0–8.0 and with 3–6 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain KX20305T grouped with members of the genus Aequorivita , including Aequorivita aquimaris D-24T (98.3 % sequence similarity), Aequorivita vladivostokensis KMM 3516T (98.1 %) and Aequorivita echinoideorum CC-CZW007T (97.5 %). Genome sequencing of strain KX20305T revealed a genome size of 3.35 Mb and a DNA G+C content of 38.71 mol%. Genomic average nucleotide identity (orthoANI) values of strain KX20305T with A. aquimaris D-24T, A. vladivostokensis KMM 3516T and A. echinoideorum JCM 30378T were 83.8, 81.7 and 75.4 %, respectively, while in silico DNA–DNA hybridization (GGDC) values for strain KX20305T with these strains were 27.2, 25.0 and 19.6 %, respectively. The major fatty acids of strain KX20305T were iso-C15 : 0, iso-C17 : 0 3-OH and 10-methyl C16 : 0/iso-C17 : 1 ω9c. The predominant respiratory quinone was menaquinone-6 (MK-6). The polar lipids mainly comprised phosphatidylethanolamine, two unidentified aminolipids and two unidentified lipids. Based on comparative analysis of phylogenetic, phylogenomic, phenotypic and chemotaxonomic characteristics, strain KX20305T represents a novel species of the genus Aequorivita , for which the name Aequorivita iocasae sp. nov. is proposed. The type strain is KX20305T (=KCTC 82699T=MCCC 1K06238T=JCM 34635T).

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Adaptive characteristics of indigenous microflora in an organically contaminated high salinity groundwater

Wang,

Zhang,

Ding

et al. 2024

Chemosphere

0

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Aequorivita sinensis sp. nov., isolated from sediment of the East China Sea, and reclassification of Vitellibacter todarodis as Aequorivita todarodis comb. nov. and Vitellibacter aquimaris as Aequorivita aquimaris comb. nov.

Cited by 14 publications

References 35 publications

Luteirhabdus pelagi gen. nov., sp. nov., a novel member of the family Flavobacteriaceae, isolated from the West Pacific Ocean

Luteirhabdus pelagi gen. nov., sp. nov., a novel member of the family Flavobacteriaceae, isolated from the West Pacific Ocean

Aequorivita iocasae sp. nov., a halophilic bacterium isolated from sediment collected at a cold seep field in the South China Sea

Adaptive characteristics of indigenous microflora in an organically contaminated high salinity groundwater

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