Vitamins C and E treatment combined with exercise modulates oxidative stress markers in blood of patients with fibromyalgia: A controlled clinical pilot study

Abstract

We aimed to investigate effects of vitamins C and E (VCE) supplementation with exercise (EX) on antioxidant vitamin and lipid peroxidation (LP) levels in blood of patients with fibromyalgia (FM). A controlled study was performed on blood samples from 32 female FM patients and 30 age-matched controls. The patients were divided into three groups namely EX (n = 10), VCE (n = 11), and EX plus VCE (n = 11) after taking basal blood samples. After 12 weeks of EX and VCE supplementation, blood samples were taken once more from the patients. LP levels in plasma and erythrocytes were higher in the patients at baseline than those in controls, whereas LP levels were lower in the VCE and EX groups at the end of 12 weeks than those at baseline. Plasma concentrations of vitamins A and E and reduced glutathione were lower in the patients than those in controls and their concentrations were increased by VCE and EX. Glutathione peroxidase activity in erythrocytes was increased by VCE supplementation, with or without EX. Concentrations of β-carotene in the groups did not change with treatment. Despite the measured effects on anti-oxidative mechanisms, FM symptoms were not improved by the treatments. In conclusion, VCE with EX may protect against FM-induced oxidative stress by up-regulation of an antioxidant redox system in the plasma and erythrocytes of patients with FM. Such protective effects of VCE in the patients seemed to be greater in combination with EX than EX alone.

Vitamin and mineral status in chronic fatigue syndrome and fibromyalgia syndrome: A systematic review and meta-analysis

“…They found no significant difference between the placebo and treatment group on any of the outcome measures. A third RCT examined vitamin C and E treatment combined with exercise versus exercise only, in FMS patients, over a period of 12 weeks [57]. Although both interventions lead to significantly higher vitamin A, C, and E serum levels, the FMS symptoms did not improve in both groups.…”

Iron oxide nanoparticles induced cytotoxicity, oxidative stress and DNA damage in lymphocytes

“…The induction of ROS was correlated with the concentration of NPs in cells and is due to the interaction of NPs with other cellular biomolecules (Park et al, ). IONPs produce free radical species that reduce the levels of cellular antioxidants significantly, which may lead to apoptosis via oxidative damage to intracellular proteins, DNA and increased LPO (Belozerskaia and Gessler, ; Ahmad et al, ; Naziroglu et al, ; Wan et al, ). Previous studies by Konczol et al () and Kawanishi et al () have reported ROS generation in A549 and CHO cells treated with magnetite NPs.…”

Ascorbic acid inhibits TPA-induced HL-60 cell differentiation by decreasing cellular H2O2 and ERK phosphorylation

“…3), which suggested that H 2 O 2 may be associated with HL-60 cells differentiation by TPA. Anti-oxidative functions of ascorbic acid have been demonstrated (35)(36)(37)(38), therefore, the present study further determined whether ascorbic acid reduces the levels of H 2 O 2 in TPA-treated HL-60 cells. The results revealed that a low-dose of ascorbic acid inhibited the increased levels of H 2 O 2 levels the TPA-treated HL-60 cells (Fig.…”

“…A previous study demonstrated that H 2 O 2 may be a secondary messenger associated with cell differentiation (25). Several studies have demonstrated that ascorbic acid exerts anti-oxidative stress functions (35)(36)(37)(38) and a previous study demonstrated that ascorbic acid decreases levels of cellular H 2 O 2 (39). Previous studies have also reported that the ERK pathway is required for TPA-differentiated HL-60 cells (13,14).…”