Vitamin D3-loaded electrospun cellulose acetate/polycaprolactone nanofibers: Characterization, in-vitro drug release and cytotoxicity studies

Wsoo, Mohammed Ahmad; Razak, Saiful Izwan Abd; Bohari, Siti Pauliena Mohd; Shahir, Shafinaz; Salihu, Rabiu; Kadir, Mohammed Rafiq Abdul; Nayan, Nadirul Hasraf Mat

doi:10.1016/j.ijbiomac.2021.03.108

Cited by 50 publications

(30 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Following that, 10 mL of MTT reagent at a 5 mg/mL concentration was added to each well and incubated for 4 h. The purple formazan was dissolved by adding 100 µL dimethyl sulfoxide to each well, including the control (which underwent no treatment), gently swirling to thoroughly mix, and then allowing it to stand at room temperature for about 30 min. The absorbance was measured at 570 nm using a microplate reader [ 30 , 31 ].…”

Section: Methodsmentioning

confidence: 99%

Electrospun Biomimetic Multifunctional Nanofibers Loaded with Ferulic Acid for Enhanced Antimicrobial and Wound-Healing Activities in STZ-Induced Diabetic Rats

et al. 2022

View full text Add to dashboard Cite

Diabetic foot ulceration is the most distressing complication of diabetes having no standard therapy. Nanofibers are an emerging and versatile nanotechnology-based drug-delivery system with unique wound-healing properties. This study aimed to prepare and evaluate silk-sericin based hybrid nanofibrous mats for diabetic foot ulcer. The nanofibrous mats were prepared by electrospinning using silk sericin mixed with different proportions of polycaprolactone (PCL) and cellulose acetate (CA) loaded with ferulic acid (FA). The in vitro characterizations, such as surface morphology, mechanical properties, swelling behavior, biodegradability, scanning electron microscopy, and drug release were carried out. The SEM images indicated that nanofibers formed with varied diameters, ranging from 100 to 250 nm, and their tensile strength was found to range from 7 to 15 MPa. In vitro release demonstrated that the nanofibers sustained FA release over an extended time of period. In vitro cytotoxicity showed that the nanofibers possessed a lower cytotoxicity in HaCaT cells. The in vivo wound-healing studies demonstrated an excellent wound-healing efficiency of the nanofibers in diabetic rats. Furthermore, the histopathological studies showed the nanofibers’ ability to restore the skin’s normal structure. Therefore, it was concluded that the prepared silk-sericin-based hybrid nanofibers loaded with FA could be a promising drug-delivery platform for the effective treatment of diabetic foot ulcers.

show abstract

Section: Methodsmentioning

confidence: 99%

Electrospun Biomimetic Multifunctional Nanofibers Loaded with Ferulic Acid for Enhanced Antimicrobial and Wound-Healing Activities in STZ-Induced Diabetic Rats

et al. 2022

View full text Add to dashboard Cite

show abstract

“…The CFU/mL were calculated at 0 h, 6 h and 24 h after plating of 100 µL of appropriate dilution factor on a Luria Bertani agar plate (for P. aeruginosa) and Tryptic soy agar plate (for S. aureus). All plates were incubated at 37 • C and CFU (colony forming unit) was counted after 24 h. Both the experiments were performed in a triplicate manner for statistically significant data between nanofibers, positive and negative control [27].…”

Section: Time-kill Assaymentioning

confidence: 99%

“…Vials plugged with cotton and open served as positive and negative controls, respectively. Microbial growth was measured by the visible spectroscopy at 600 nm wavelength and colony forming unit [27]. In 75 cm 2 flasks, human keratinocytes (HaCaT, NCCS, Pune, India) were grown in full Dulbecco's Modified Eagle's medium (DMEM), which consisted of DMEM medium with 10% heat-inactivated fetal bovine serum and 1% L-glutamine.…”

Section: Microbial Penetration Testmentioning

confidence: 99%

Multifunctional Biomimetic Nanofibrous Scaffold Loaded with Asiaticoside for Rapid Diabetic Wound Healing

et al. 2022

View full text Add to dashboard Cite

Diabetes mellitus is a chronic disease with a high mortality rate and many complications. A non-healing diabetic foot ulcer (DFU) is one the most serious complications, leading to lower-extremity amputation in 15% of diabetic patients. Nanofibers are emerging as versatile wound dressing due to their unique wound healing properties, such as a high surface area to volume ratio, porosity, and ability to maintain a moist wound environment capable of delivering sustained drug release and oxygen supply to a wound. The present study was aimed to prepare and evaluate a polyvinyl alcohol (PVA)–sodium alginate (SA)–silk fibroin (SF)-based multifunctional nanofibrous scaffold loaded with asiaticoside (AT) in diabetic rats. The SEM findings showed that fibers’ diameters ranged from 100–200 nm, and tensile strengths ranged from 12.41–16.80 MPa. The crosslinked nanofibers were sustained AT over an extended period. The MTT and scratch assay on HaCat cells confirmed low cytotoxicity and significant cell migration, respectively. Antimicrobial tests revealed an excellent anti-microbial efficacy against P. aeruginosa and S. aureus bacteria. In-vivo study demonstrated better wound healing efficacy in diabetic rats. In addition, the histopathological studies showed its ability to restore the normal structure of the skin. The present study concluded that developed multifunctional nanofibers have a great potential for diabetic wound healing applications.

show abstract

“…Certainly, based on the core-sheath nanostructures, a wide variety of drugs may be delivered through their OM dosage forms when fast actions are needed to relieve pain or bring down a fever. Besides drug delivery, the protocols reported here should be also useful for delivering nutrition in food science and engineering and for cosmetic applications [82][83][84].…”

Section: Strategy For Developing Medicated Membrane Using Modified Coaxial Electrospinningmentioning

confidence: 99%

Orodispersible Membranes from a Modified Coaxial Electrospinning for Fast Dissolution of Diclofenac Sodium

Ning

Zhou

et al. 2021

Membranes

View full text Add to dashboard Cite

The dissolution of poorly water-soluble drugs has been a longstanding and important issue in pharmaceutics during the past several decades. Nanotechnologies and their products have been broadly investigated for providing novel strategies for resolving this problem. In the present study, a new orodispersible membrane (OM) comprising electrospun nanofibers is developed for the fast dissolution of diclofenac sodium (DS). A modified coaxial electrospinning was implemented for the preparation of membranes, during which an unspinnable solution of sucralose was explored as the sheath working fluid for smoothing the working processes and also adjusting the taste of membranes. SEM and TEM images demonstrated that the OMs were composed of linear nanofibers with core-sheath inner structures. XRD and ATR-FTIR results suggested that DS presented in the OMs in an amorphous state due to the fine compatibility between DS and PVP. In vitro dissolution measurements and simulated artificial tongue experiments verified that the OMs were able to release the loaded DS in a pulsatile manner. The present protocols pave the way for the fast dissolution and fast action of a series of poorly water-soluble active ingredients that are suitable for oral administration.

show abstract

Vitamin D3-loaded electrospun cellulose acetate/polycaprolactone nanofibers: Characterization, in-vitro drug release and cytotoxicity studies

Cited by 50 publications

References 62 publications

Electrospun Biomimetic Multifunctional Nanofibers Loaded with Ferulic Acid for Enhanced Antimicrobial and Wound-Healing Activities in STZ-Induced Diabetic Rats

Electrospun Biomimetic Multifunctional Nanofibers Loaded with Ferulic Acid for Enhanced Antimicrobial and Wound-Healing Activities in STZ-Induced Diabetic Rats

Multifunctional Biomimetic Nanofibrous Scaffold Loaded with Asiaticoside for Rapid Diabetic Wound Healing

Orodispersible Membranes from a Modified Coaxial Electrospinning for Fast Dissolution of Diclofenac Sodium

Contact Info

Product

Resources

About