Vitamin D binding protein, a new nutritional marker in cystic fibrosis patients

Speeckaert, Marijn M.; Wehlou, Charline; Vandewalle, Sara; Taes, Youri; Robberecht, Eddy; Delanghe, Joris

doi:10.1515/cclm.2008.084

Cited by 40 publications

(29 citation statements)

References 22 publications

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“…GC-2 has the lowest affinity for 25OHD while GC-1S has twofold greater affinity and GC-1F has a fourfold increase in affinity for 25OHD relative to GC-2 (Arnaud & Constans 1993). At present, it is not clear whether there is any connection between these affinity differences and reported correlations between DBP polymorphisms and thyroid disorders, diabetes, obesity, and other diseases (White & Cooke 2000, Speeckaert et al 2008.…”

Section: Effects Of Dbp Gene Variants On Human Physiology and Evolutionmentioning

confidence: 97%

Back to the future: a new look at ‘old’ vitamin D

Chun¹,

Adams²,

Hewison³

2008

Journal of Endocrinology

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Our perception of the vitamin D system continues to evolve. Recent studies have re-evaluated the parameters for adequate vitamin D status in humans, revealing a high prevalence of insufficiency in many populations throughout the world. Other reports have highlighted the potential consequences of vitamin D insufficiency beyond established effects on bone homeostasis. Most notably, there is now strong evidence of a role for vitamin D in modulating innate and adaptive immunities, with insufficiency being linked to infectious disease and other immune disorders. To date, signaling pathways for these new responses to vitamin D have been based on established endocrine models for active 1,25-dihydroxyvitamin D, despite present evidence for more localized, intracrine modes of action. In the following review, we provide a fresh perspective on vitamin D signaling in nonclassical target cells such as macrophages by highlighting novel factors associated with the transport and action of this pluripotent secosteroid.

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Section: Effects Of Dbp Gene Variants On Human Physiology and Evolutionmentioning

confidence: 97%

Back to the future: a new look at ‘old’ vitamin D

Chun¹,

Adams²,

Hewison³

2008

Journal of Endocrinology

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“…First of all, this member of the albumin superfamily of binding proteins is a passive carrier and an active facilitator of the cellular uptake of vitamin D and its metabolites. 1 The large molar excess of DBP in relation to its major ligand 25(OH)-vitamin D 3 opens the possibility to act as a circulating vitamin D reservoir. Besides the well-described variety of DBP phenotypes (three common alleles, together with numerous other variants) and genotypes, little attention has been paid to the association of this plasma protein with the various lipoprotein fractions.…”

Section: Introductionmentioning

confidence: 99%

“…2 As we have reported previously, a correlation between serum DBP concentration and lipid parameters (serum total cholesterol, low-density lipoprotein [LDL]-cholesterol, triglycerides and leptin concentration) was found in cystic fibrosis patients. 3 The distribution of the partially lipophilic DBP in plasma and its potential association with lipoprotein particles and vitamin D has only been investigated to a small degree. Using ultracentrifugation, Haddad et al analysed upper and lower phases for 25(OH)-vitamin D 3 , 1.25(OH) 2 -vitamin D 3 , cholesterol and DBP.…”

Section: Introductionmentioning

confidence: 99%

“…3 The distribution of the partially lipophilic DBP in plasma and its potential association with lipoprotein particles and vitamin D has only been investigated to a small degree. Using ultracentrifugation, Haddad et al analysed upper and lower phases for 25(OH)-vitamin D 3 , 1.25(OH) 2 -vitamin D 3 , cholesterol and DBP. Forty-one percent of 25(OH)-vitamin D 3 and .85% of cholesterol was detected at density ,1.3 g/ mL, whereas nearly all (.95%) 1.25(OH) 2 -vitamin D 3 and DBP was found at density .1.3 g/mL.…”

Section: Introductionmentioning

confidence: 99%

“…Using ultracentrifugation, Haddad et al analysed upper and lower phases for 25(OH)-vitamin D 3 , 1.25(OH) 2 -vitamin D 3 , cholesterol and DBP. Forty-one percent of 25(OH)-vitamin D 3 and .85% of cholesterol was detected at density ,1.3 g/ mL, whereas nearly all (.95%) 1.25(OH) 2 -vitamin D 3 and DBP was found at density .1.3 g/mL. 4 However, 88% of 25(OH)-vitamin D 3 is bound to DBP with high affinity…”

Section: Introductionmentioning

confidence: 99%

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Investigation of the potential association of vitamin D binding protein with lipoproteins

et al. 2010

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Background: Vitamin D binding protein (DBP) acts as a vitamin D carrier and an actin scavenger. We have previously reported a correlation between serum DBP and lipid parameters in a cystic fibrosis population. In the present study, the relationship between serum DBP, lipoprotein fractions and vitamin D is investigated. Methods: The presence of DBP in lipoprotein fractions was examined using precipitation, gel permeation chromatography and ultracentrifugation. The association between DBP and lipids was investigated in a cohort study of 211 men. Total and actin-free DBP concentrations were assessed by immunonephelometry and enzyme-linked immunosorbent assay. Serum 25(OH)-and 1.25(OH) 2 -vitamin D 3 were assayed by radioimmunoassay. Total cholesterol, high-density lipoprotein cholesterol (HDL), triglycerides and insulin were measured using routine methods. Low-density lipoprotein-cholesterol (LDL) was calculated according to Friedewald's formula. Results: DBP was found to be present in very-low-density lipoprotein (VLDL). Gel permeation chromatography revealed a bimodal DBP distribution with a lipid-bound fraction besides the known free fraction. Ultracentrifugation confirmed the presence of DBP and 25(OH)-vitamin D 3 in the VLDL particle. Total serum DBP concentration and the actin-bound DBP/DBP ratio correlated significantly with total cholesterol, LDL-cholesterol, triglycerides and albumin. The 25(OH)-vitamin D 3 /DBP ratio correlated negatively with serum triglyceride concentration and body mass index (BMI). The actin-bound DBP complex was identified with Western blot. Conclusions: The lipid-bound DBP fraction may be of greater importance than initially thought. The present findings may have clinical consequences in view of the important physiological role of DBP.

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