Vitamin C - vitamin E interaction in juvenile lake sturgeon (Acipenser fulvescens R.), a fish able to synthesize ascorbic acid

Abstract

Summary The hypothesis that vitamin C interacts with vitamin E in vivo was investigated in juvenile lake sturgeon. Ten‐month old lake sturgeon were fed diets supplemented with either 0 or 1250 mg ascorbic acid/kg diet concomitantly with either 0 or 200 mg α tocopherol/kg diet for 7 weeks at 17°C. Dietary vitamin C supplement resulted in significant increases of ascorbate concentrations in the posterior kidney and liver of sturgeon. Dietary vitamin E omission affected liver concentrations of α‐tocopherol (10.0 ± 4.5 μg/g) in comparison to sturgeon fed a diet supplemented with vitamin E and vitamin C (99.5 ± 22.9 μg/g). Dietary vitamin C supplement decreased liver α‐tocopherol concentration in vitamin E‐deprived sturgeon. Also, vitamin E supplement lowered posterior kidney and liver ascorbic acid concentrations in vitamin C‐deprived sturgeon. Gulonolactone oxidase and dehydroascorbic acid reductase activities were stimulated in groups fed vitamin C. Thiobarbituric acid‐reactive substances concentrations (an indicator of lipid peroxidation) were higher in sturgeon fed either of vitamins as compared to sturgeon deprived of both vitamins. The results suggested that large doses of vitamins C and E may be prooxidant in vivo.

Antioxidant Defenses in Fish: Biotic and Abiotic Factors

“…In Atlantic salmon (Salmo salar), Hamre et al (1997) reported two different interacting mechanisms: regeneration of vitamin E from its oxidized form by vitamin C, and a synergistic effect of simultaneous protection of the water and lipid phases against oxidation. This synergistic effect of vitamins E and C against oxidation has also been observed in lake sturgeon (Acipenser fulvescens) (Moreau et al, 1999), yellowtail (Seriola quinqueradiata) (Ito et al, 1999) and hybrid tilapia (Oreochromis spp.) (Shiau and Hsu, 2002).…”

Effects of dietary vitamin C supplementation on performance, tissue chemical composition and alkaline phosphatase activity in great sturgeon (Huso huso)

“…After feeding juvenile lake sturgeon (Acipenser fulvescens) with a diet containing 1250 mg Testing the effects of Vitamin C in diets during the rearing of juvenile Great sturgeon (Huso huso) in tanks for 16 weeks. Data represent mortality counts from three replicates with an initial stocking density of 55 fish per tank ascorbic acid in the diet, the concentration of ascorbate in liver and posterior kidney significantly increased, but the increase of gulonolactone oxidase was not significant (Moreau et al, 1999b) and growth and survival were not significantly affected by dietary treatment after 7 weeks of feeding. The most important reason for the lack of significant effects on growth parameters is the existence of L-gulono-1, 4-lactone oxidase in sturgeon fish (Maeland and Waagbo, 1998;Moreau et al, 1999a;Dabrowski, 2001).…”

Beneficial roles of feed additives as immunostimulants in aquaculture: a review

“…), juvenile lake sturgeon ( Acipenser fulvescens ) (Moreau et al . ), channel catfish (Lim et al . ), yellow perch ( Perca flavescens ) (Lee & Dabrowski ) and red sea bream ( P. major ) (Gao et al .…”