Vitamin a Assays of Plant Tissues: Potential Sources of Error in Sampling

Booher, Lela E.; Hewston, Elizabeth M.; Marsh, Rosemary Loughlin

doi:10.1111/j.1365-2621.1941.tb16307.x

Cited by 6 publications

(1 citation statement)

References 3 publications

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“…PRELIMINARY OBSERVATIONS ' Most plant materials, when fresh, contain about 80% of moisture; hence, any method of direct extraction of carotene should be preceded by drying, for which purpose preliminary blanching, and use of alcohol, acetone, and water-binding agents like anhydrous sodium sulfate, sodium carbonate, and calcium sulfate have been recommended (3,8,17,26,41,42,48). As freshly ignited lime is a good desiccant as well as adsorbent for chlorophyll and xanthophylls, a number of experiments were carried out in which known amounts of leaf were macerated in a mortar and further triturated with varying proportions of freshly prepared lime, chemically pure and ignited for a few minutes at a temperature of 250°to 300°C.…”

Section: Methodsmentioning

confidence: 99%

Determination of Carotene in Plant Materials

Sreenivasan¹,

Vaidya²

1948

Anal. Chem.

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A rapid method for the routine determination of carotene in plant materials has been worked out by which the green tissues are triturated with a mixture of lime and anhydrous sodium sulfate. This results in their disintegration as well as adsorption of chlorophyll and other noncarotenoid pigments; the carotene itself is rendered readily extractable by petroleum ether or benzene and can be determined either as gross carotene or as its isomerides after chromatographic resolution. The method is particularly suitable for the analysis of green, leafy materials, feeds, and fodders and gives excellent agreement with standard procedures, but it can be applied with equal success to fresh fruits and vegetables.

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