Colorimetric Determination of Copper with Carbon Disulfide and Diethanolamine

Woelfel, Warren C.

doi:10.1021/ac60020a009

Cited by 25 publications

(9 citation statements)

References 40 publications

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“…For example, bis-HE-DTC was formed quantitatively by the in situ condensation of diethanolamine and CS 2 ,21 and observed to be stable at pH 6 or pH 10 for at least several days when protected from air. Furthermore, bis-HE-DTC was only mildly sensitive to air oxidation, with less than 10% change in absorbance intensity after a 6-hour exposure (see Figure 4a).…”

Section: Resultsmentioning

confidence: 99%

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Assembly of Dithiocarbamate-Anchored Monolayers on Gold Surfaces in Aqueous Solutions

et al. 2008

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Dithiocarbamates (DTCs) can be formed by the in situ condensation of polar alkylamines with CS 2 , and assembled into dithiocarbamate-anchored monolayers (DAMs) on Au substrates in aqueous solutions. Primary and secondary amines can both be used to prepare DTCs, but have significant differences in their reactivities and product stabilities. Ultraviolet absorption spectroscopy provides a convenient method for monitoring in situ DTC formation as well as the formation of potential byproducts. The kinetics of DAM assembly on Au substrates as measured by second harmonic generation (SHG) indicated first-order rate processes and saturation coverages similar to those of alkanethiols on Au. However, the rate of adsorption did not change with DTC concentration in a manner expected of Langmuir kinetics, and is attributed to the competitive adsorption of alkylammonium counterions to the freshly oxidized Au substrate. These analyses establish a practical range of conditions for preparing DAMs from polar amines using in situ DTC formation.

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Section: Resultsmentioning

confidence: 99%

“…A wide variety of DTCs can be prepared in situ by the condensation of CS 2 with nucleophilic amines, using mildly basic conditions and polar solvents 13,21. This simple condensation can be conducted either offline or in the presence of Au substrates, permitting DAMs to be assembled from amines in a single step.…”

Section: Introductionmentioning

confidence: 99%

Assembly of Dithiocarbamate-Anchored Monolayers on Gold Surfaces in Aqueous Solutions

et al. 2008

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“…Copper lost from the IUDs was estimated spectrophotometrically (Woelfel, 1948) as previously described (Tobert, 1974).…”

Section: Determination Ofcopper Lossmentioning

confidence: 99%

Effect of copper and platinum intrauterine devices on endometrial morphology and implantation in the rabbit

Tobert¹,

Davies²

1977

Reproduction

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“…Copper binding to fibrinogen was determined by the equilibrium dialysis technique described previously for heparin-copper systems. The total inside copper concentrations at 25.0"C were determined spectrophotometrically using Woelfel's reagent, 25 and the initial fibrinogen concentration was determined from the absorption at 280 mp while the final soluble protein concentration was obtained from the fringe shift pattern in the analytical ultracentrifuge using interference optics.…”

Section: Copper Binding Studiesmentioning

confidence: 99%

Copper ion binding and heparin interactions of human fibrinogen

Lages

Stivala

1973

Biopolymers

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synopsisThe binding of copper ions to fibrinogen was studied by the equilibrium dialysis technique in neutral Tris buffer. The presence of copper causes precipitation of fibrinogencopper complexes, the amount of which varies with the copper ion concentration. Solutions of 96% clottable fibrinogen (whole fibrinogen) displayed a maximum binding capacity about four times greater than that of fibrinogen solutions from which the coldinsoluble precipitate had been removed (cold-soluble fibrinogen). Binding in both systems apparently involves two classes of sites in fibrinogen, and the class of lower affinity is associated with cooperative interactions with copper. The copper concentration a t which this cooperative uptake occurs is identical to the concentration at which the amount of precipitated material increases sharply and also to the concentration at which a sharp decrease is observed in the sedimentation coefficient of soluble fibrinogen, suggesting some relationship between copper binding, solubility, and solution properties.The presence of heparin markedly affects the sedimentation coefficient of fibrinogen in the presence of copper ions, although showing a lesser effect in the absence of the metal. The sedimentation coefficient of fibrinogen is increased in the presence of heparin and copper ion, compared to the value of fibrinogen-copper systems without heparin, and this effect is enhanced by changing the fibrinogen: heparin molar ratio to larger values. The precipitation of fibrinogen from solution, apparently without a coincident removal of heparin, also increases with increasing copper ion concentration and fibrinogen:heparin molar ratio. The possible significance of these effects in terms of heparin anti-coagulant activity is discussed. * Submitted (by B. L.) in partial fulfillment of the requirements for thePh.D. degree,

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Colorimetric Determination of Copper with Carbon Disulfide and Diethanolamine

Abstract: samples could be dealt lvith.food blender, cutter, or chopper (27) AIo~toli, R. A., "Application of Absorption Spectra to the Study of Vitamins, Hormones and Co-enzymes." pp. 61, 103, London. Adam Hilrar. 1942. and the refluxing procedure with solvents are unnecessary.

Cited by 25 publications

References 40 publications

Assembly of Dithiocarbamate-Anchored Monolayers on Gold Surfaces in Aqueous Solutions

Assembly of Dithiocarbamate-Anchored Monolayers on Gold Surfaces in Aqueous Solutions

Effect of copper and platinum intrauterine devices on endometrial morphology and implantation in the rabbit

Copper ion binding and heparin interactions of human fibrinogen

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