Visualization of gold and platinum nanoparticles interacting with Salmonella Enteritidis and Listeria monocytogenes

Abstract

Purpose: Rapid development of nanotechnology has recently brought significant attention to the extraordinary biological features of nanomaterials. The objective of the present investigation was to evaluate morphological characteristics of the assembles of gold and platinum nanoparticles (nano-Au and nano-Pt respectively), with Salmonella Enteritidis (Gram-negative) and Listeria monocytogenes (Gram-positive), to reveal possibilities of constructing bacteria-nanoparticle vehicles. Methods: Hydrocolloids of nano-Au or nano-Pt were added to two bacteria suspensions in the following order: nano-Au + Salmonella Enteritidis; nano-Au + Listeria monocytogenes; nano-Pt + Salmonella Enteritidis; nano-Pt + Listeria monocytogenes. Samples were inspected by transmission electron microscope. Results: Visualization of morphological interaction between nano-Au and Salmonella Enteritidis and Listeria monocytogenes, showed that nano-Au were aggregated within flagella or biofilm network and did not penetrate the bacterial cell. The analysis of morphological effects of interaction of nano-Pt with bacteria revealed that nano-Pt entered cells of Listeria monocytogenes and were removed from the cells. In the case of Salmonella Enteritidis, nano-Pt were seen inside bacteria cells, probably bound to DNA and partly left bacterial cells. After washing and centrifugation, some of the nano-Pt-DNA complexes were observed within Salmonella Enteritidis. Conclusion:The results indicate that the bacteria could be used as a vehicle to deliver nano-Pt to specific points in the body.

Organic resolution function and effects of platinum nanoparticles on bacteria and organic matter

“…In a similar experiment that was previously reported, nanoplatinum particles (2–19 nm) were allowed to react for 15 minutes. These nanoparticles exhibited the same levels of antibacterial activity as that reported in our study, even though the bacterial strains were different [31,32]. The reaction time of the solution used and platinum particle size were almost the same, and the results obtained were similar to those reported in our study.…”

“…Furthermore, PtNPs, which are clusters of Pt atoms in the range of 1–100 nm, are attracting attention due to the extremely high catalytic activity. Recently, the ability of these nanoparticles to suppress inflammation has also been evaluated [17,18]. Chemical interactions are thought to be induced by contact between bacteria and PtNPs, leading to the decomposition of bacteria.…”

Interaction of graphene family materials with Listeria monocytogenes and Salmonella enterica

“…In our previous studies, we examined how interactions of Salmonella enterica and Listeria monocytogenes with various nanoparticles (diamond, silver, gold, and platinum) affected bacterial morphology [ 7 , 24 ]. In this work, we examined how three different graphene nanostructures affect the chosen food-borne bacteria strains: the Gram-positive (G+) L. monocytogenes and Gram-negative (G−) S. enterica .…”

Characterization and bacterial anti-adherent effect on modified PMMA denture acrylic resin containing platinum nanoparticles

“…The antibacterial activity of Pt has been known since the work of Rosenberg et al,11 who reported its inhibitory activity on Escherichia coli division. Significantly, Pt nanoparticles (PtN), cluster of Pt atoms with sizes ranging from 1 to 100 nm, are of great interest owing to their highly catalytic activity and are currently being evaluated for the ability to reduce inflammation 10,12. Contact between PtN and bacteria promotes chemical interactions that cause bacterial cell to be disintegrated 13.…”