Virulence genes of Escherichia coli in diarrheic and healthy calves

Umpiérrez, Ana; Ernst, Déborah; Fernández, M. Fernández; Oliver, Martín; Casaux, María Laura; Caffarena, Rubén D.; Schild, Carlos; Giannitti, Federico; Fraga, Martín; Zunino, Pablo

doi:10.1016/j.ram.2020.04.004

Cited by 11 publications

(19 citation statements)

References 9 publications

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“…In Uruguay, the proportion of STEC in the faeces of calves is low, according to previous studies of our group (Umpiérrez et al, 2017(Umpiérrez et al, , 2021. However, given the severity and outcome of the illness, the regional context of HUS cases in children, and the ecology of STEC transmission and persistence in the environment it is required to find out the potentially harmful effects of circulating STEC isolates.…”

Section: Introductionmentioning

confidence: 78%

“…Sixteen STEC strains isolated from bovine faeces were collected between 2014 and 2017. As previously described, faeces collection and biochemical and molecular characterisation of E. coli were performed (Umpiérrez et al, 2017(Umpiérrez et al, , 2021. Briefly, all isolates except one were collected from faeces of seven dairy calves with signs of neonatal calf diarrhoea (NCD) and from faeces of dairy calves without NCD signs under 35 days old (table 1), whereas one isolate was collected from an ileum sample of a dead calf affected with NCD (table 1).…”

Section: Isolates and Growth Conditionsmentioning

confidence: 99%

“…Briefly, all isolates except one were collected from faeces of seven dairy calves with signs of neonatal calf diarrhoea (NCD) and from faeces of dairy calves without NCD signs under 35 days old (table 1), whereas one isolate was collected from an ileum sample of a dead calf affected with NCD (table 1). All isolates were previously characterised by PCR, regarding the presence of stx1, stx2, and eae among other E. coli virulence genes, and classified as STEC LEE+ (stx+/eae+) (Umpiérrez et al, 2017(Umpiérrez et al, , 2021. For the routine cultivation of STEC, isolates were grown on trypticase soy agar (TSA) plates (OXOID) for 18-24 h at 37 ºC.…”

Section: Isolates and Growth Conditionsmentioning

confidence: 99%

See 2 more Smart Citations

Non-O157 Shiga toxin-producing Escherichia coli with potential harmful profiles to humans are isolated from the faeces of calves in Uruguay

Umpiérrez

Ernst

Cardozo

et al. 2022

Austral j. vet. sci.

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Section: Introductionmentioning

confidence: 78%

Section: Isolates and Growth Conditionsmentioning

confidence: 99%

Section: Isolates and Growth Conditionsmentioning

confidence: 99%

See 1 more Smart Citation

Non-O157 Shiga toxin-producing Escherichia coli with potential harmful profiles to humans are isolated from the faeces of calves in Uruguay

Umpiérrez

Ernst

Cardozo

et al. 2022

Austral j. vet. sci.

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“…However, other bacterial pathogens that may cause severe intestinal, colonic and/or cecal damage such as attaching and effacing -enteropathogenic and enterohemorrhagic- E. coli , were not assessed for in this study. A study on virulence genes of E. coli isolated from diarrheic and non-diarrheic dairy calves in Uruguay, using samples of the dairy calves of this study, found a poor representation of genes associated with the shiga toxin-producing E. coli (STEC) / enterohemorrhagic E. coli (EHEC) group, as well as enteropathogenic E. coli (EPEC) [ 69 ]. Interestingly, a study on postmortem findings and laboratory-based diagnosis of causes of death in dairy calves in the USA found that calves with necrotizing and ulcerative intestinal lesions were more likely to be diagnosed with rotavirus infection [ 70 ].…”

Section: Discussionmentioning

confidence: 99%

Causes of neonatal calf diarrhea and mortality in pasture-based dairy herds in Uruguay: a farm-matched case-control study

et al. 2021

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Neonatal calf diarrhea (NCD) and mortality cause significant losses to the dairy industry. The preweaning dairy calf mortality risk in Uruguay is high (15.2%); however, causes for these losses are largely unknown. This study aimed to assess whether various pathogens were associated with NCD and death in Uruguayan dairy calves and whether these infections, diarrhea, or deaths were associated with the failure of transfer of passive immunity (FTPI). Contemporary diarrheic (n = 264,) and non-diarrheic (n = 271) 1-to 30-day-old calves from 27 farms were sampled. Feces were analyzed by antigen-capture ELISA for Cryptosporidium spp., rotavirus, bovine coronavirus, and Escherichia coli F5+, RT-PCR for bovine astrovirus (BoAstV), and bacterial cultures for Salmonella enterica. Blood/serum was analyzed by RT-PCR or antigen-capture ELISA for bovine viral diarrhea virus (BVDV). Serum of ≤ 8-day-old calves (n = 95) was assessed by refractometry to determine the concention of serum total proteins (STP) as an indicator of FTPI. Whether the sampled calves died before weaning was recorded. At least one pathogen was detected in 65.4% of the calves, and this percentage was significantly higher in diarrheic (83.7%) versus non-diarrheic (47.6%) calves. Unlike the other pathogens, Cryptosporidium spp. and rotavirus were associated with NCD. Diarrheic calves, calves infected with any of the pathogens, and calves infected with rotavirus had significantly lower concentrations of STP. Diarrheic calves had higher chances of dying before weaning than non-diarrheic calves. Diarrheic calves infected with S. enterica were at increased risk of mortality. Controlling NCD, salmonellosis, cryptosporidiosis, and rotavirus infections, and improving colostrum management practices would help to reduce calf morbi-mortality in dairy farms in Uruguay.

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“…La enterobacteria Escherichia coli (E. coli), bacteria Gram negativa, no formadora de esporas y móvil por flagelos perítricos, es un microrganismo que se encuentra colonizando el tracto gastrointestinal de animales y humanos lo que facilita su diseminación por medio de las heces (Umpiérrez et al, 2021;Sanchez, 2019).…”

Section: Introductionunclassified

Genes y expresión de factores de virulencia en Escherichia coli aislada en animales de producción

2022

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Los factores de virulencia de patógenos se expresan una vez superan los mecanismos fisiológicos de respuesta inmune por parte del organismo, Escherichia coli (E. coli) es una bacteria que se considera un problema en salud pública en todo el mundo debido a la alta prevalencia de mecanismos de resistencia y patogenicidad que expresa, se relaciona principalmente con infecciones intestinales y es transmitida en la cadena alimenticia; los determinantes genéticos que codifican factores de virulencia se trasladan entre diferentes especies o la misma, mediante el mecanismo de transferencia horizontal de genes. El objetivo de la presente revisión es describir factores de virulencia y genes que los codifican en cepas de E. coli aisladas de animales de producción y de productos alimenticios. En las bases de datos Medline, Lilacs, ScienceDirect, Scopus, SciELO y Dialnet, se realizó búsqueda utilizando una combinación de palabras claves validadas en inglés (gen, virulence, virulence factor, infection, horizontal gene transfer, mutation and production animals). En diferentes ambientes de producción se identificó la presencia de diferentes mecanismos de virulencia que varían según la especie animal y bacteriana, los genes con mayor circulación con Stx1, Stx2 codificantes de toxinas, Saa de adhesinas, ehxA de enterohemolisina, eaeA de intimina, IpfA de fimbrias. La vigilancia y control microbiológico en el área alimenticia y de producción animal es de gran importancia para evitar posibles brotes de enfermedades en población susceptible, por efecto de los factores de virulencia.

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Virulence genes of Escherichia coli in diarrheic and healthy calves

Cited by 11 publications

References 9 publications

Non-O157 Shiga toxin-producing Escherichia coli with potential harmful profiles to humans are isolated from the faeces of calves in Uruguay

Non-O157 Shiga toxin-producing Escherichia coli with potential harmful profiles to humans are isolated from the faeces of calves in Uruguay

Causes of neonatal calf diarrhea and mortality in pasture-based dairy herds in Uruguay: a farm-matched case-control study

Genes y expresión de factores de virulencia en Escherichia coli aislada en animales de producción

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