Virucidal activity of Moringa A from Moringa oleifera seeds against Influenza A Viruses by regulating TFEB

Abstract

Background In uenza A viruses (IAVs) are highly contagious pathogens infecting human and numerous animals. The viruses cause millions of infection cases and thousands of deaths every year, thus making IAVs a continual threat to global health. Methods Moringa A was isolated from Moringa oleifera seeds and tested for antiviral activity against H1N1. The antiviral activity of Moringa A was tested by checking their effect on hemagglutination and PFU activities of the studied virus, and the cytopathic effect was observed too. Additionally, the different types of treatment experiments were performed to complement the analysis of the antiviral activity of Moringa A, and the contents of in ammatory cytokines and the expression of TFEB were detected. Results Moringa A inhibits virus replication in host cells, and it protects infected cells from cytopathic effect induced by IAVs. The EC50 and EC90 value of Moringa A for IAVs were 1.27 and 5.30 μM, respectively. The different types of treatment experiments revealed that Moringa A has a signi cant inhibitory effect on the IAVs both before and after drug addition. What's more, Moringa A was observed to decrease the levels of in ammatory cytokines TNF-α, IL-6, IL-1β and IFN-β in H1N1 infected RAW264.7 cells. Finaly, Moringa A was found to inhibit the expression and nuclear transfer of the cellular protein transcription factor EB (TFEB), and weaken the autophagy in infected cells, which could be an important antiviral mechnism of Moringa A. Conclusions Moringa A has potent antiviral activity against IVAs, which could be due to the autophagy inhibition property.

Effect of Cytokinins on Micropropagation of Moringa Oleifera Lam. and Genomic Stability Assessment Using Flow-Cytometry

“…Its leaves, owers and fresh pods can be consumed as vegetables as well as utilized as livestock feed, making it valuable from the food security point of view (Anjorin et al, 2010). Reportedly, Moringa leaves are endowed with β-carotene, protein, vitamin C, calcium and potassium and act as a good source of natural antioxidants such as avonoids, phenolics and carotenoids (Mehwish et al, 2021;Xiong et al, 2021;Daghaghele et al, 2021). On the other hand, various parts of the horseradish tree such as the leaves, root, bark, seed, owers, and immature pods are found to have medicinal properties.…”

The Characteristics of Liquid Soap with Additional Variations of Moringa Seed Extract (Moringa oleifera L.)

“…moringa seeds are known to contain antibacterial compounds such as flavonoids, alkaloids, saponins, and tannins [4] [25]. Based on these contents, moringa seeds have the potential to be used as an active antibacterial ingredient [26]. However, not many studies have used Moringa seed extract as an active ingredient, especially liquid soap.…”

ESTUDIOS QUÍMICOS REALIZADOS A LA SEMILLA DE Moringa oleifera Lam Y SU IMPACTO EN LA SALUD HUMANA: UNA REVISIÓN TEÓRICA

“…Dentro del fruto de Moringa oleífera se encuentran las semillas, que se caracterizan por el alto contenido de ácidos grasos, proteínas, vitaminas, fibra, y compuestos fenólicos, alcaloides, glucosilonatos, los cuales le confieren actividad cicatrizante antimicrobiana, antioxidantes (Alí et al, 2021;Xiong et al, 2021;Kwabena et al, 2020) y antinflamatoria (Domínguez et al, 2014).…”

“…Aplicaciones.CHEMICAL STUDIES CARRIED OUT ON THE SEED OF Moringa oleifera Lam AND ITS IMPACT ON HUMAN HEALTH: A THEORETICAL REVIEWABSTRACT: The purpose of this research was to investigate the chemical studies carried out on the Moringa oleifera Lam seed, analyzing its impact on human health. This research had a qualitative, descriptive approach with a documentary design based on authors such asGiuberti et al, (2021); Ali et al, (2021);Kapse & Samadder, (2021);Batista et al, (2021);Gharsallah et al, (2021);Ogusku et al, (2021);Xiong et al, (2021);Kwabena et al, (2020);Gu, Yang & Wang, (2020);…”

“…Ciência e Tecnologia: Desenvolvimento Ambiental, Cultural e Socioeconômico 1 Los autores expresan que no tienen conflicto de intereses.RESUMEN: , (2021);Alí et al, (2021);Kapse & Samadder, (2021);Batista et al, (2021);Gharsallah et al, (2021);Ogusku et al, (2021);Xiong et al, (2021);Kwabena et al, (2020); Gu, Yang & Wang, (2020);…”