Viral loads in clinical samples of men with monkeypox virus infection: a French case series

Palich, Romain; Burrel, Sonia; Monsel, Gentiane; Nouchi, A.; Bleibtreu, A.; Seang, Sophie; Bérot, V.; Brin, Cécile; Gavaud, Ariane; Wakim, Yara; Godefroy, N.; Fayçal, Antoine; Tamzali, Yanis; Grunemwald, Thomas; Ohayon, Michel; Todesco, Eve; Leducq, Valentin; Marot, Stéphane; Cálvez, Vincent; Marcelin, Anne‐Geneviève; Pourcher, Valérie

doi:10.1016/s1473-3099(22)00586-2

Cited by 116 publications

(180 citation statements)

References 21 publications

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“…Anogenital swabs are reported to be the second highest viral load site in mpox, after skin lesions. 1 Viral load in the anal swab of the patient (PCR cycle threshold 26·8) was consistent with the presence of replication-competent virus. 4 Moreover, monkeypox virus isolation from a urethral swab of an asymptomatic patient was reported, supporting contagiousness of asymptomatically infected people via sexual contacts.…”

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confidence: 64%

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People with asymptomatic or unrecognised infection potentially contribute to monkeypox virus transmission

Accordini¹,

Cordioli²,

Pomari³

et al. 2023

The Lancet Microbe

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mentioning

confidence: 64%

“…Studies published in 2022 have shown monkeypox virus genome detection in multiple body sites from infected patients. 1 , 2 Furthermore, multiple PCR-positive samples have been reported in asymptomatic individuals. 3 The contribution of people with asymptomatic or subclinical infection to the monkeypox virus transmission chain should be established.…”

mentioning

confidence: 99%

People with asymptomatic or unrecognised infection potentially contribute to monkeypox virus transmission

Accordini¹,

Cordioli²,

Pomari³

et al. 2023

The Lancet Microbe

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“… 32 , 34 , 77 , 78 Moreover, the viral load in skin samples is higher by about two orders of magnitude compared with that in other body locations (viral load 10 6 copies per mL vs 10 4 copies per mL; cycle threshold values approximately 22 vs approximately 28). 77 , 78 , 79 , 80 In addition, evidence of replication-competent virus isolation has been reported more frequently from skin samples than from throat swabs and semen samples. 78 , 80 Altogether these data support that intimate sexual contact is the main route of transmission, with the respiratory route playing a less important role.…”

Section: Transmissionmentioning

confidence: 99%

Monkeypox

Mitjà¹,

Ogoina²,

Titanji³

et al. 2023

The Lancet

275

276

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“…The history of previous or concurrent sexually-transmitted infections (STIs) among MPX-confirmed cases in included studies is highlighted in Table 2. These STIs included human immunodeficiency virus (overall, 265/635 cases), 6,8,[13][14][15][18][19][20] Herpes simplex virus (4/378 cases), 6,19 Chlamydia trachomatis (21/389), 6,15 Neisseria gonorrhea (33/389), 6,15 and syphilis (39/394). 6,15…”

Section: Baseline Characteristicsmentioning

confidence: 99%

Monkeypox viral detection in semen specimens of confirmed cases: A systematic review and meta‐analysis

Reda

Abdelaal

Brakat

et al. 2022

Journal of Medical Virology

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Background The current literature shows increasing concerns about potential seminal transmission of monkeypox virus (MPV). Accordingly, we aimed to understand better the potential presence of MPV in the seminal uids and others specimens obtained from monkeypox (MPX) cases.Methods On June 26, 2022, a systematic search of the literature was conducted across PubMed, Scopus, Web of Science, EMBASE, ScienceDirect, ProQuest, EBSCOHost-Academic Search Complete, and Google Scholar to nd articles that examine the presence of MPV in seminal uid of con rmed cases . The search was updated on August 12 to include newly published articles. The prevalence of MPV DNA presence in the seminal uid and other specimens was pooled in a meta-analysis and results were presented as effect sizes and their corresponding 95% con dence intervals (CI). The quality of included articles was assessed using the National Institute of Health tool.Results Eight articles (including 585 MPX-con rmed patients) were included. Only four studies were eligible for a meta-analysis, and the individual positivity rate of MPV DNA in semen specimens ranged from 61.11% to as high as 90.62%, while the pooled rate was 78% (95% CI: 62-93%; I2=61.93%) among 91 examined patients. Moreover, the pooled positive rate of MPV DNA was the highest in rectal samples (100%; 95%CI: 94-100%), followed by urinary (31%; 95%CI: 1-61%), nasopharyngeal (28%; 95%CI: 24-32%), and blood/plasma (8%; 95%CI: 6-10%) samples, respectively. Furthermore, two articles also investigated the infectivity of MPV particles detected in seminal specimens by testing their replication competence. Culturing MPV was successful in one out of three patients included in these studies. Also, based on available evidence, the positivity of MPV in semen specimens can be observed early and up to 19 days after symptoms onset.Conclusions MPV is highly prevalent in seminal specimens of MPX cases, further corroborating the role of sexual transmission of the disease. However, further evidence is still needed to shed more light on the replication competence of these particles.

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Viral loads in clinical samples of men with monkeypox virus infection: a French case series

Cited by 116 publications

References 21 publications

People with asymptomatic or unrecognised infection potentially contribute to monkeypox virus transmission

People with asymptomatic or unrecognised infection potentially contribute to monkeypox virus transmission

Monkeypox

Monkeypox viral detection in semen specimens of confirmed cases: A systematic review and meta‐analysis

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