Vibrio parahaemolyticus virulence determinants

Santos, Marcela de Souza; Salomon, Dor; Krachler, Anne Marie; Orth, Kim

doi:10.1016/b978-0-12-800188-2.00008-2

Cited by 15 publications

(16 citation statements)

References 149 publications

(299 reference statements)

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“…Over the last decade, enormous progress has been made to elucidate virulence factors used by this pathogen and tools are available to study virulence mechanisms at the genetic and biochemical levels ( de Souza Santos et al, 2015 ). As part of its virulence repertoire, V. parahaemolyticus encodes two T3SSs: the cytotoxic T3SS1 and the enterotoxic T3SS2.…”

Section: Introductionmentioning

confidence: 99%

Bile salt receptor complex activates a pathogenic type III secretion system

Rivera-Cancel

Kinch

et al. 2016

eLife

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124

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Bile is an important component of the human gastrointestinal tract with an essential role in food absorption and antimicrobial activities. Enteric bacterial pathogens have developed strategies to sense bile as an environmental cue to regulate virulence genes during infection. We discovered that Vibrio parahaemolyticus VtrC, along with VtrA and VtrB, are required for activating the virulence type III secretion system 2 in response to bile salts. The VtrA/VtrC complex activates VtrB in the presence of bile salts. The crystal structure of the periplasmic domains of the VtrA/VtrC heterodimer reveals a β-barrel with a hydrophobic inner chamber. A co-crystal structure of VtrA/VtrC with bile salt, along with biophysical and mutational analysis, demonstrates that the hydrophobic chamber binds bile salts and activates the virulence network. As part of a family of conserved signaling receptors, VtrA/VtrC provides structural and functional insights into the evolutionarily conserved mechanism used by bacteria to sense their environment.DOI: http://dx.doi.org/10.7554/eLife.15718.001

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Section: Introductionmentioning

confidence: 99%

Bile salt receptor complex activates a pathogenic type III secretion system

Rivera-Cancel

Kinch

et al. 2016

eLife

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124

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“…For example, overexpression of HflKC complex stabilizes CII (key tetrameric transcription factor for lysogenic establishment) in vivo in E. coli , causing an increase in lysogenic frequency . There is little information regarding the flagellar system of pathogenic V. parahaemolyticus strain; however, it is considered a virulence determinant in this species ; for example, mutagenized flagellin causes significant loss of virulence in Vibrio anguillarum . It is unclear if differential expression of these types of proteins has biological implications regarding a bacterium's performance.…”

Section: Discussionmentioning

confidence: 99%

Proteomic profiling of integral membrane proteins associated to pathogenicity in Vibrio parahaemolyticus strains

Pérez-Acosta

Martínez‐Porchas

Elizalde‐Contreras

et al. 2018

Microbiology and Immunology

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Vibrio parahaemolyticus has been recognized as the causal agent of early mortality syndrome and is currently considered an emerging shrimp disease causing losses of millions in the aquaculture industry. Integral membrane proteins are widely recognized as pathogenicity factors involved in essential mechanisms for V. parahaemolyticus infection, which makes them attractive as therapeutic targets. However, their physico-chemical properties and weak expression has resulted in underrepresentation of these proteins in conventional bottom-up proteomics, making integral membrane proteomics a challenging task. Integral membrane proteins from a bacterial strain isolated from the hepatopancreases of white shrimp with early mortality syndrome and identified by 16S rRNA sequencing as V. parahaemolyticus and an ATCC strain that is pathogenic for humans were obtained by a sequential extraction method and subjected to relative quantification and identification by isobaric Tags for Relative and Absolute Quantitation. A homology database search resulted in identification of more than two hundred proteins, 35 of which are recognized as pathogenic factors showed statistically significant differential accumulation between the strains. These proteins are mainly associated with adherence, secretion systems, cell division, transport, lysogenization, movement and virulence. Identification of pathogenicity-related proteins in V. parahaemolyticus provides valuable information for developing strategies based on molecular mechanisms that inhibit these proteins, which may be useful therapeutic targets for assisting the shrimp and aquaculture industry.

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“…RTX proteins can also play a role in host protection as bacteriocins or by forming protective bacterial surface layers (S-layers) (Linhartová et al, 2010). RTX proteins exhibit additional biological activities as metalloproteases, lipases, pore-forming toxins, iron-regulated proteins, nodulation-related proteins and are involved both in bacterial adherence/motility and host-receptor interactions (Welch, 2001;de Souza Santos et al, 2015). RTX proteins are secreted by a T1SS via Sec-independent pathway used by gramnegative bacteria to transport proteins from the cytoplasm to the extracellular medium in a single step (Chenal et al, 2015).…”

Section: Putative Roles Played By Toxinsmentioning

confidence: 99%

Genomic Attributes of Novel Symbiont Pseudovibrio brasiliensis sp. nov. Isolated From the Sponge Arenosclera brasiliensis

et al. 2018

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Sponge holobionts are defined as the host animals and their associated microbiomes. Both host and microbiome produce extracellular products that facilitate interaction within the holobiont. For example, microbes may provide nutrition for the animal host and protection against pathogens. The genomic study of bacterial cultures may shed light on the properties of novel symbiotic bacteria isolated from marine holobionts. In this study, we performed a genome-based analysis of Pseudovibrio brasiliensis Ab134 T isolated from the sponge Arenosclera brasiliensis. This novel strain is phylogenetically related to Pseudovibrio denitrificans. In silico DNA-DNA hybridization and calculation of the average amino acid identity between the strain Ab134 T and P. denitrificans JCM 12308 T showed <70% similarity and <95% identity, respectively. This novel bacterial species possesses genomic features that hint at several possible roles in symbiosis (e.g., production of secondary metabolites, including bromotyrosine-derived alkaloids) in sponge and coral holobionts. We also detected gene clusters encoding type III, type IV, and type VI secretion systems and 26 toxin-like proteins, including probable paralogs. Our results demonstrate the genome versatility of P. brasiliensis Ab134 T and the potential to attach to host cells, which may play a role in its symbiotic lifestyle.

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Vibrio parahaemolyticus virulence determinants

Cited by 15 publications

References 149 publications

Bile salt receptor complex activates a pathogenic type III secretion system

Bile salt receptor complex activates a pathogenic type III secretion system

Proteomic profiling of integral membrane proteins associated to pathogenicity in Vibrio parahaemolyticus strains

Genomic Attributes of Novel Symbiont Pseudovibrio brasiliensis sp. nov. Isolated From the Sponge Arenosclera brasiliensis

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