Genomic Attributes of Novel Symbiont Pseudovibrio brasiliensis sp. nov. Isolated From the Sponge Arenosclera brasiliensis

Abstract

Sponge holobionts are defined as the host animals and their associated microbiomes. Both host and microbiome produce extracellular products that facilitate interaction within the holobiont. For example, microbes may provide nutrition for the animal host and protection against pathogens. The genomic study of bacterial cultures may shed light on the properties of novel symbiotic bacteria isolated from marine holobionts. In this study, we performed a genome-based analysis of Pseudovibrio brasiliensis Ab134 T isolated from the sponge Arenosclera brasiliensis. This novel strain is phylogenetically related to Pseudovibrio denitrificans. In silico DNA-DNA hybridization and calculation of the average amino acid identity between the strain Ab134 T and P. denitrificans JCM 12308 T showed <70% similarity and <95% identity, respectively. This novel bacterial species possesses genomic features that hint at several possible roles in symbiosis (e.g., production of secondary metabolites, including bromotyrosine-derived alkaloids) in sponge and coral holobionts. We also detected gene clusters encoding type III, type IV, and type VI secretion systems and 26 toxin-like proteins, including probable paralogs. Our results demonstrate the genome versatility of P. brasiliensis Ab134 T and the potential to attach to host cells, which may play a role in its symbiotic lifestyle.

A novel family of nonribosomal peptides modulate collective behavior in Pseudovibrio bacteria isolated from marine sponges

“…16 . A draft genome of strain Ab134 obtained using Illumina sequencing was previously reported (GenBank accession number: MIEL00000000) 17 . In order to generate a complete genome, we repeated short-read sequencing with Illumina and also obtained long-read Nanopore data.…”

Cultivation of Bacteria From Aplysina aerophoba: Effects of Oxygen and Nutrient Gradients

“…Amongst these shared taxa, the alphaproteobacterial Pseudovibrio and Ruegeria OTUs were the most frequently cultivated. Pseudovibrio species represent low abundant sponge symbionts which are known as versatile, opportunistic bacteria capable to adapt to a wide range of cultivation conditions (Muscholl-Silberhorn et al, 2008;Bondarev et al, 2013;Versluis et al, 2017;Fróes et al, 2018). With this study we could furthermore add aeroplysinin-1 to the list of antibiotic resistances exhibited by Pseudovibrio species (Versluis et al, 2017).…”

Ecological and biotechnological aspects of Aplysina-associated microorganisms

“…Amongst these shared taxa, the Alphaproteobacteria Pseudovibrio and Ruegeria OTUs were the most frequently cultivated and grew on almost every cultivation method applied in this study. Pseudovibrio species represent low abundant sponge symbionts which are known as versatile, opportunistic bacteria capable to adapt to a wide range of cultivation conditions (Muscholl-Silberhorn et al, 2008;Bondarev et al, 2013;Versluis et al, 2017;Fróes et al, 2018). With this study we could furthermore add aeroplysinin-1 to the list of antibiotic resistances exhibited by Pseudovibrio species (Versluis et al, 2017).…”

“…This alphaproteobacterial lineage has been repeatedly isolated from sponges and also detectable with molecular methods (Chapter 4). It appears to be a commensalistic or even beneficial symbiont with the genomic potential to attach and interact with eukaryotic cells, supply the host with vitamins and cofactors and produce sponge-specific secondary metabolites (Bondarev et al, 2013;Nicacio et al, 2017;Alex & Antunes, 2018;Fróes et al, 2018;Versluis et al, 2018). Its facultative anaerobic lifestyle, fast growth rate and wide substrate utilization capabilities furthermore render Pseudovibrio species valuable candidates for industrial upscaling in order to produce spongederived secondary metabolites.…”