Vibrio parahaemolyticus strains isolated during investigation of the summer 2006 seafood related diarrhea outbreaks in two regions of Chile

Fuenzalida, Loreto; Armijo, Lorena; Zabala, Beatriz; Hernández, Cristina; Rioseco, María Luisa; Riquelme, Carlos; Espejo, Romilio T.

doi:10.1016/j.ijfoodmicro.2007.03.011

Cited by 55 publications

(40 citation statements)

References 27 publications

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“…Only the pandemic strain was isolated from clinical samples before and during the summer of 2006, but 20 different strains were isolated from shellfi sh during that period (2). Characterization of 52 isolates from 20 shellfi sh samples during the summer of 2007 indicated ≥5 DGREA groups; 3 of them were not previously observed ( Table 2; Figure 2).…”

Section: Parahaemolyticus Associated With Shellfi Shmentioning

confidence: 89%

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Epidemiology ofVibrio parahaemolyticusOutbreaks, Southern Chile

Harth

Matsuda²,

Hernández³

et al. 2009

Emerg. Infect. Dis.

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Disease outbreaks caused by Vibrio parahaemolyticus in Puerto Montt, Chile, began in 2004 and reached a peak in 2005 at 3,600 clinical cases. Until 2006, every analyzed case was caused by the serovar O3:K6 pandemic strain. In the summer of 2007, only 475 cases were reported; 73% corresponded to the pandemic strain. This decrease was associated with a change in serotype of many pandemic isolates to O3:K59 and the emergence of new clinical strains. One of these strains, associated with 11% of the cases, was genotypically different from the pandemic strain but contained genes that were identical to those found on its pathogenicity island. These fi ndings suggest that pathogenicity-related genes were laterally transferred from the pandemic strain to one of the different V. parahaemolyticus groups comprising the diverse and shifting bacterial population in shellfi sh in this region.

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Section: Parahaemolyticus Associated With Shellfi Shmentioning

confidence: 89%

“…However, the pandemic strain is a minor fraction of a diverse and shifting V. parahaemolyticus population found in shellfi sh in Puerto Montt (2). In an effort to understand the epidemiology of these outbreaks, we studied V. (4) and reported in this article.…”

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confidence: 99%

Epidemiology ofVibrio parahaemolyticusOutbreaks, Southern Chile

Harth

Matsuda²,

Hernández³

et al. 2009

Emerg. Infect. Dis.

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“…3 Sendo que algumas espécies de Vibrio, como o Vibrio parahaemolyticus, são também patógenos humanos, causando distúrbios entéricos. 4 Os crustáceos são providos apenas de um sistema imune inato ou natural, diferentemente dos vertebrados, que possuem além deste, um sistema adaptativo. 5 Diversos autores têm demonstrado que o fornecimento de substâncias imunoestimulantes aos camarões, pode melhorar os seus índices imunológicos, resultando em uma maior resistência à infecção por patógenos.…”

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Avaliação de bacterina e Lactobacillus plantarum frente à infecção experimental por Vibrio harveyi em pós-larvas de Litopenaeus vannamei

Buglione¹,

Pedrotti²,

Vieira³

et al. 2008

Braz. J. Vet. Res. Anim. Sci.

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ResumoObjetivou-se averiguar o efeito da bactéria Lactobacillus plantarum e células inativas das bactérias Vibrio alginolyticus, Aeromonas salmonicida e Pasteurella multocida na sobrevivência larval de Litoenaeus vannamei, no teste de estresse e infecção experimental com Vibrio harveyi. Foram utilizados tanques cônicos de 30 L, povoados com 400 larvas cada, no estádio de pós-larva cinco. Tratamentos em triplicatas foram consistidos de: 1: ração comercial (controle), 2: ração comercial + bacterina via oral na artemia, 3: ração comercial + bacterina via imersão e 4: ração comercial com inóculo de Lactobacillus plantarum. A aplicação da bacterina ocorreu seis horas antes da infecção e do teste de estresse; enquanto o Lactobacillus plantarum foi administrado por 15 dias antes dos desafios. As pós-larvas do tratamento 4 (ração suplementada com L. plantarum) obtiveram maior índice de sobrevivência no teste de estresse (87,86 ± 2,35%), seguido dos tratamentos 2 e 3 (bacterina via imersão e oral) com 81,54±1,50% e 80,16 ± 2,15% respectivamente, superiores ao índice do controle (72,63 ± 3,34%). Já no desafio com V. harveyi, os animais do grupo tratado com a adição de bacterina via imersão apresentaram maior sobrevivência (79,60 ± 7,12%). As pós-larvas dos tratamentos com bacterina via oral na artêmia e alimentadas com o probiótico L. plantarum, apresentaram sobrevivências de 65,60 ± 5,18% e 69,60 ± 10,43 %, respectivamente, sendo superiores ao controle (56,4 ± 5,58%), quando desafiados com V. harveyi. Os resultados demonstram que o uso de ração com L. plantarum e bacterina aumentam a sobrevivência das pós-larvas de L. vannamei frente aos testes de estresse e infecções experimentais com V. harveyi. Palavras-chaves:Camarão marinho. Vibriose. Probiótico. Bactérias. IntroduçãoConsiderando a indústria aqüícola no mundo e no Brasil, as enfermidades em organismos aquáticos, representam um dos principais fatores de risco. 1 Em geral, o cultivo de camarões na América tem sofrido baixas devido às enfermidades de origem viral e bacteriana.As doenças bacterianas são causadas principalmente por bactérias do gênero Vibrio. 2 A septicemia causada por vibrios e as infecções internas localizadas são acompanhadas por diferentes sinais clínicos como opacidade da musculatura abdominal, anorexia e cromatóforos expandidos. 3 Sendo que algumas espécies de Vibrio, como o Vibrio parahaemolyticus, são também patógenos humanos, causando distúrbios entéricos. 4 Os crustáceos são providos apenas de um sistema imune inato ou natural,

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“…Phage VP58.5 was isolated from a V. parahaemolyticus strain belonging to the serovar O3:K6 pandemic clonal complex (41). During the last several years, this clone has been associated with many seafood-borne diarrhea outbreaks in Southeast Asia and South America, particularly Chile (5,12,13,15). Up to 33% of the Chilean isolates harbored a 42-kb plasmid which was shown to be the prophage of a myovirus inducible by mitomycin C. VP58.5 is the prototype of these phages.…”

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confidence: 99%

The Linear Plasmid Prophage Vp58.5 of Vibrio parahaemolyticus Is Closely Related to the Integrating Phage VHML and Constitutes a New Incompatibility Group of Telomere Phages

Zabala

Hammerl

Espejo

et al. 2009

J Virol

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Vibrio parahaemolyticus O3:K6 pandemic strains recovered in Chile frequently possess a 42-kb plasmid which is the prophage of a myovirus. We studied the prototype phage VP58.5 and show that it does not integrate into the host cell chromosome but replicates as a linear plasmid (Vp58.5) with covalently closed ends (telomeres). The Vp58.5 replicon coexists with other plasmid prophages (N15, PY54, and ⌽KO2) in the same cell and thus belongs to a new incompatibility group of telomere phages. We determined the complete nucleotide sequence (42,612 nucleotides) of the VP58.5 phage DNA and compared it with that of the plasmid prophage. The two molecules share the same nucleotide sequence but are 35% circularly permuted to each other. In contrast to the hairpin ends of the plasmid, VP58.5 phage DNA contains 5-protruding ends. The VP58.5 sequence is 92% identical to the sequence of phage VHML, which was reported to integrate into the host chromosome. However, the gene order and termini of the phage DNAs are different. The VHML genome exhibits the same gene order as does the Vp58.5 plasmid. VHML phage DNA has been reported to contain terminal inverted repeats. This repetitive sequence is similar to the telomere resolution site (telRL) of VP58.5 which, after processing by the phage protelomerase, forms the hairpin ends of the Vp58.5 prophage. It is discussed why these closely related phages may be so different in terms of their genome ends and their lifestyle.Most temperate bacteriophages integrate into the host chromosome during lysogeny. However, there are some phages (telomere phages) whose prophages are linear plasmids with covalently closed ends. Members of this group of phages are the siphoviruses N15, PY54, and ⌽KO2 isolated from Escherichia coli, Yersinia enterocolitica, and Klebsiella oxytoca, respectively, and the recently described myoviruses ⌽HAP-1 of Halomonas aquamarina and VP882 of Vibrio parahaemolyticus (6,20,23,26,37). Despite their different origins (enterobacteria versus marine bacterium) and morphologies, all known telomere phages share similar genome organizations and some protein similarities. The linear DNA of each phage is a circular permutation of the respective linear plasmid prophage. For the generation of the terminal hairpins of the linear plasmid, the protelomerase (Tel) is essential (8). This enzyme has cleaving/joining activity; its target is a large palindromic DNA sequence called the telomere resolution site (telRL) located upstream of tel on the phage genome. After cleaving telRL by staggered cuts, the resulting self-complementary single-stranded DNA overhangs fold back and are rejoined by the protelomerase (9). Besides tel, all telomere phages possess the gene repA, encoding a multifunctional replication protein. repA of N15 and PY54 was shown to harbor the prophage replication origin and to function as a circular minimal replicon (35,42). Compatibility studies demonstrated that the N15 and ⌽KO2 plasmids belong to the same incompatibility group, whereas the PY54 plasmid is able to coexist...

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Vibrio parahaemolyticus strains isolated during investigation of the summer 2006 seafood related diarrhea outbreaks in two regions of Chile

Cited by 55 publications

References 27 publications

Epidemiology ofVibrio parahaemolyticusOutbreaks, Southern Chile

Epidemiology ofVibrio parahaemolyticusOutbreaks, Southern Chile

Avaliação de bacterina e Lactobacillus plantarum frente à infecção experimental por Vibrio harveyi em pós-larvas de Litopenaeus vannamei

The Linear Plasmid Prophage Vp58.5 of Vibrio parahaemolyticus Is Closely Related to the Integrating Phage VHML and Constitutes a New Incompatibility Group of Telomere Phages

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