Vibrational Spectroscopic Imaging of Hard Tissues

Morris, Michael D.; Schulmerich, Matthew V.; Dooley, Kathryn A.; Esmonde-White, Karen A.

doi:10.1002/9783527628230.ch4

Cited by 12 publications

(13 citation statements)

References 112 publications

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“…To characterize the collagen maturation using Raman spectroscopy, the amide I band is used as a cross-link parameter as follows: the collagen cross-link ratio (nonreducible band at 1660 cm -1 to reducible band at 1690 cm -1 ) represents the collagen maturation indicator [25,27]. As described above, this calculation has been disputed [28].…”

Section: Micro-raman Spectroscopy Of Hard Tissuesmentioning

confidence: 99%

“…In the IR spectrum of a bone, the mineral-to-matrix ratio, which indicates the mineral content and the degree of calcification, is calculated as the ratio of the integrated ν1, ν3 phosphate envelope to the amide I envelope [25]. The carbonate-tophosphate ratio is calculated as the ratio of the ν2 carbonate envelope to that of the integrated ν1, ν3 phosphate envelope.…”

Section: Ftir Imaging Of Hard Tissuesmentioning

confidence: 99%

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Studies on bone metabolism by using isotope microscopy, FTIR imaging, and micro-Raman spectroscopy

Kimura-Suda

Kajiwara

Sakamoto

et al. 2013

Journal of Oral Biosciences

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Section: Micro-raman Spectroscopy Of Hard Tissuesmentioning

confidence: 99%

Section: Ftir Imaging Of Hard Tissuesmentioning

confidence: 99%

Studies on bone metabolism by using isotope microscopy, FTIR imaging, and micro-Raman spectroscopy

Kimura-Suda

Kajiwara

Sakamoto

et al. 2013

Journal of Oral Biosciences

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“…Its use in bone studies ranges from investigations of the compositional properties of materials [20,21,22,23] to biomechanical applications [24,25,26] and the evaluation of bone quality [27,28,29,30]. …”

Section: Introductionmentioning

confidence: 99%

A Bone Sample Containing a Bone Graft Substitute Analyzed by Correlating Density Information Obtained by X-ray Micro Tomography with Compositional Information Obtained by Raman Microscopy

et al. 2015

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The ability of bone graft substitutes to promote new bone formation has been increasingly used in the medical field to repair skeletal defects or to replace missing bone in a broad range of applications in dentistry and orthopedics. A common way to assess such materials is via micro computed tomography (µ-CT), through the density information content provided by the absorption of X-rays. Information on the chemical composition of a material can be obtained via Raman spectroscopy. By investigating a bone sample from miniature pigs containing the bone graft substitute Bio Oss®, we pursued the target of assessing to what extent the density information gained by µ-CT imaging matches the chemical information content provided by Raman spectroscopic imaging. Raman images and Raman correlation maps of the investigated sample were used in order to generate a Raman based segmented image by means of an agglomerative, hierarchical cluster analysis. The resulting segments, showing chemically related areas, were subsequently compared with the µ-CT image by means of a one-way ANOVA. We found out that to a certain extent typical gray-level values (and the related histograms) in the µ-CT image can be reliably related to specific segments within the image resulting from the cluster analysis.

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“…Bone strength, which is a barometer of bone health, primarily reflects the integration of two features: bone quantity (bone mineral density) and bone quality [2,3]. FTIR imaging is a powerful tool for the characterization of bone quality, including parameters such as the mineral-to-collagen matrix ratio, carbonateto-phosphate ratio in the hydroxyapatite, crystallinity, and collagen maturity [1,[4][5][6][7]. In our previous work [1], we used FTIR imaging to characterize the bone quality of the femur in a rat model of CKD induced by 5/6 nephrectomy (Nx).…”

Section: Introductionmentioning

confidence: 99%

Collagen Fiber Orientation in the Femur of Rats with Chronic Kidney Disease

Kimura-Suda

Ito

Kanazawa

et al. 2015

e-J. Surf. Sci. Nanotech.

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Collagen fiber orientation in the femur of chronic kidney disease (CKD) rats was determined using Fourier transform infrared (FTIR) imaging with a wire grid polarizer in order to characterize bone quality in CKD. Elevenweek-old male rats underwent 5/6 nephrectomy (Nx) to replicate CKD. These CDK rats, as well as sham-operated rats (sham), were kept for 16 weeks. After sacrifice, femurs in the sham and CKD rats were removed and embedded in PMMA. Longitudinal sections (∼3 µm) of the femurs were prepared for FTIR imaging. IR dichroism images were calculated from the FTIR images obtained using two polarized IR beams (0 • and 90 • ). The IR dichroism images of the collagen fibers in the CKD rat femurs indicated that the collagen fibers in the cortical bone were aligned longitudinally, and the degree of collagen fiber orientation was decreased in the metaphysis compared to that in the diaphysis. The carbonate-to-phosphate ratio in the hydroxyapatite was reduced in both the cortical bone and trabecular bone of the CKD rat femurs. In the cortical bone, reductions in the carbonate-to-phosphate ratio and collagen fiber orientation were particularly evident in the metaphysis in the CKD rats.

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Vibrational Spectroscopic Imaging of Hard Tissues

Cited by 12 publications

References 112 publications

Studies on bone metabolism by using isotope microscopy, FTIR imaging, and micro-Raman spectroscopy

Studies on bone metabolism by using isotope microscopy, FTIR imaging, and micro-Raman spectroscopy

A Bone Sample Containing a Bone Graft Substitute Analyzed by Correlating Density Information Obtained by X-ray Micro Tomography with Compositional Information Obtained by Raman Microscopy

Collagen Fiber Orientation in the Femur of Rats with Chronic Kidney Disease

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